Molecular confirmation &amp; characterization of Rickettsia conorii in north India: A report of three cases

Biswal, Manisha; Zaman, Kamran; Suri, Vikas; Gopi, Srikanth; Kumar, Abhay; Gopi, T; Vig, Shashi; Sharma, Navneet; Bhalla, Ashish

doi:10.4103/ijmr.ijmr_92_18

Cited by 10 publications

(13 citation statements)

References 26 publications

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“…In a previous study from our hospital of 51 patients who were negative for the known causes of fever, three were diagnosed to have spotted fever due to Rickettsia conorii [8]. In the present study, our hypothesis was that there might be a bigger burden of rickettsial etiology of AFI.…”

Section: Introductionmentioning

confidence: 63%

Rickettsial Diseases: Not Uncommon Causes of Acute Febrile Illness in India

et al. 2020

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Rickettsial diseases (RDs) are major under-diagnosed causes of arthropod borne acute febrile illness (AFI) presenting with a range of symptoms from mild self-limiting fever to fatal sepsis. The spotted fever group (SFG) and typhus group (TG) are major RDs, which are commonly caused by Rickettsia conorii and Rickettsia typhi, respectively. The limited availability and role of serological tests in the acute phase of illness warrants rapid reliable molecular methods for diagnosis and epidemiological studies. Two hundred patients with AFI in whom the routine fever diagnostics were negative, were enrolled over a period of two months (April 2019 to May 2019). DNA was extracted and in-house nested PCR using primers specific for both SPG and TG pathogens was used. The positive amplified products were sequenced for species identification and phylogenetic analysis was performed using MEGA 7.0.14 software (iGEM, Temple University, Philadelphia, PA 19122, USA). The demographic details of the RD cases were documented. The prevalence of RD among AFI cases was 7% (14/200); SFG and TG were identified as the cause in 4% and 3% of AFI cases, respectively. The median age of the RD cases was 22 years (range 2-65). The median duration of fever was 3 days (range 1-12). The RD cases presented with respiratory symptoms or signs (44.44%), jaundice (22.22%), abdominal pain (22.22%), diarrhea (22.22), vesicular rash (11.11%), vomiting (11.11%), loss of appetite (11.11%), headache (11.11%), leukocytosis (88.88% with mean count 22,750/mm 3 ), and thrombocytopenia (33.33%). The cases were treated empirically with piperacillin-tazobactam (66.66%), clindamycin (44.44%), cefotaxime (33.33%), meropenem (33.33%), metronidazole (33.33%), doxycycline (22.22%), azithromycin (22.22%), ceftriaxone (11.11%), and amoxicillin-clavulanic acid (11.11%). The mortality among the RD cases was 11.11%. The present pilot study shows that RD is not an uncommon cause of AFI in north India. The febrile episodes are usually transient, not severe and associated with heterogenous clinical presentation without documented history of tick exposure in the hospitalized patients. The transient, non-severe, febrile illness could be due to transient rickettsemia resulting from empirical antimicrobial therapy as the rickettsial organisms are expected to be more susceptible to higher doses of β-lactam antibiotics. The study emphasizes the molecular method as a useful tool to identify rickettsial etiology in AFI.

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Section: Introductionmentioning

confidence: 63%

Rickettsial Diseases: Not Uncommon Causes of Acute Febrile Illness in India

et al. 2020

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“…3 However, ITT is reported rarely in northern states of India. [4][5][6][7][8] Cutaneous manifestations of ITT usually include eschar and purpura fulminans. Gangrene in ITT is restricted to a few case reports.…”

Section: Discussionmentioning

confidence: 99%

“…Hence ITT should be kept as a differential in cases of acute febrile illness presenting with features of endothelial damage and/or vasculitis, and early initiation of doxycycline will help save many lives as well as reduce morbidity. [3][4][5][6][7][8]…”

Section: Discussionmentioning

confidence: 99%

Indian Tick Typhus Presenting as Gangrene: a Case Report

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Gonttumukkula

Samyanathan

et al. 2023

Pediatric Infectious Disease Journal

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Rickettsial infections are an important cause of acute febrile illness in developing countries. They can rarely present with cutaneous manifestations such as gangrene. Here we report a 12-year-old boy who presented with acute undifferentiated fever, multiorgan dysfunction, and gangrene of bilateral toes and ear lobes. The diagnosis was made by serologic testing of paired sera and clinical recovery occurred with doxycycline.

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“…The members of this group exhibit substantial genome sequence similarity and shared antigenic makeup; however, the diseases they cause might be distinguished by specific clinical manifestations, rates of illness or death, and the areas of their endemicity and predominant tick vectors ( 6 ). PCR-confirmed clinical cases caused by ITTR have been diagnosed in India ( 7 ), Sicily ( 8 ) and Xinjiang Uygur Autonomous Region, China (GenBank accession nos. MG190327–9).…”

Section: Discussionmentioning

confidence: 99%