Molecular composition and regulation of the Nox family NAD(P)H oxidases

Sumimoto, Hideki; Miyano, Kei; Takeya, Ryu

doi:10.1016/j.bbrc.2005.08.210

Cited by 275 publications

(294 citation statements)

References 87 publications

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“…However, this result appears to indicate that Nox4 is not involved in ionizing irradiation-induced ROS generation, although it has been reported that Nox3 and Nox4 are constitutively active and that they produce some amount of O 2 - [38,39].…”

mentioning

confidence: 55%

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Ionizing irradiation induces apoptotic damage of salivary gland acinar cells via NADPH oxidase 1-dependent superoxide generation

Tateishi

Sasabe

Ueta

et al. 2008

Biochemical and Biophysical Research Communications

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mentioning

confidence: 55%

“…Although it has been reported that Nox3 and Nox4 are constitutively active and produce a small but significant amount of O 2 - [38,39], the physiological function and regulation of the expression of Nox3 and Nox4, which are expressed in only NS-SV-DC cells and in both cell lines, respectively, in the salivary gland are currently unknown.…”

mentioning

confidence: 99%

Ionizing irradiation induces apoptotic damage of salivary gland acinar cells via NADPH oxidase 1-dependent superoxide generation

Tateishi

Sasabe

Ueta

et al. 2008

Biochemical and Biophysical Research Communications

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“…NADPH oxidase was first described in phagocytes (24), but is also expressed in a wide range of cell-types (36,41,44), including NTS neurons (48). This enzyme consists of two membrane-bound subunits, gp91 phox and p22 phox , several mobile cytoplasmic subunits including, p40 phox , and p67 phox , and the essential p47 phox , as well as the small GTPase RAC (24,38). Superoxide production by NADPH oxidase is the result of a cascade of cellular events including increased intracellular calcium, such as that induced by AngII-induced stimulation of the AT-1A receptor, leading to phosphorylation and membrane translocation of p47 phox to the gp91 phox subunit (6,40).…”

Section: Introductionmentioning

confidence: 99%

Changes in the subcellular distribution of NADPH oxidase subunit p47phox in dendrites of rat dorsomedial nucleus tractus solitarius neurons in response to chronic administration of hypertensive agents

Glass

Chan

Frys

et al. 2007

Experimental Neurology

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NADPH oxidase-generated superoxide can modulate crucial intracellular signaling cascades in neurons of the nucleus tractus solitarius (NTS), a brain region that plays an important role in cardiovascular processes. Modulation of NTS signaling by superoxide may be linked to the subcellular location of the mobile NADPH oxidase p47 phox subunit, which is known to be present in dendrites of NTS neurons. It is not known, however, if hypertension can produce changes in the trafficking of p47 phox in defined NTS subregions, particularly the preferentially barosensitive dorsomedial NTS (dmNTS), or preferentially gastrointestinal medial NTS (mNTS). We used immunogold electron microscopy to determine if p47 phox localization was differentially affected in dendritic profiles of neurons from these NTS subregions of the rat in response to distinct models of hypertension, namely chronic seven-day subcutaneous administration of angiotensin II (AngII), or phenylephrine. In small (<1 μm) dendritic processes, both AngII and phenylephrine produced a decrease in intracellular p47 phox labeling selectively in dmNTS neurons. In intermediate-size (1-2 μm) dendritic profiles in the dmNTS region only, there was an increase in p47 phox labeling in response to each hypertensive agent, although these changes occurred in different subcellular compartments. There was an increase in non-vesicular labeling in response to AngII, but an increase in surface labeling with phenylephrine. Moreover, each of the changes in p47 phox targeting mentioned above occurred in dendritic profiles with, or without immunoperoxidase labeling for the AngII AT-1A receptor subtype (AT-1A). These results indicate that chronic administration of agents that induce hypertension can also produce changes in the subcellular localization in p47 phox in dmNTS neurons. Thus, systemic hypertension may produce alterations in the trafficking of proteins associated with superoxide production in central autonomic neurons, thus revealing a potentially important neurogenic component of free radical production and systemic blood pressure elevation.

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“…This enzyme was first described in phagocytes (Lassègue and Clempus, 2003), but is now known to be present in diverse cell types (Serrano et al, 2003, Tejada-Simon et al, 2005, Vallet et al, 2005, including NTS neurons (Wang et al, 2004). NADPH oxidase is comprised of two membrane-bound subunits, gp91 phox and p22 phox , several mobile cytoplasmic subunits including, p40 phox , and p67 phox , and the essential p47 phox , as well as the small GTPase RAC (Lambeth, 2004, Sumimoto et al, 2005. NADPH oxidase activation can be triggered by intracellular calcium, such as that induced by stimulation of the AT-1A receptor subtype, with subsequent phosphorylation and membrane translocation of p47 phox to the gp91 phox subunit (Cai et al, 2003, Taniyama and.…”

Section: Introductionmentioning

confidence: 99%

Subcellular localization of nicotinamide adenine dinucleotide phosphate oxidase subunits in neurons and astroglia of the rat medial nucleus tractus solitarius: Relationship with tyrosine hydroxylase immunoreactive neurons

et al. 2006

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Superoxide produced by the enzyme NADPH oxidase mediates crucial intracellular signaling cascades in the mNTS, a brain region populated by catecholaminergic neurons, as well as astroglia that play an important role in autonomic function. The mechanisms mediating NADPH oxidase (phox) activity in the neural regulation of cardiovascular processes are incompletely understood, however the subcellular localization of superoxide produced by the enzyme is likely to be an important regulatory factor. We used immunogold electron microscopy to determine the phenotypic and subcellular localization of the NADPH oxidase subunits p47 phox , gp91 phox, and p22 phox in the mNTS. The mNTS contains a large population of neurons that synthesize catecholamines. Significantly, catecholaminergic signaling can be modulated by redox reactions. Therefore, the relationship of NADPH oxidase subunit labeled neurons or glia with respect to catecholaminergic neurons was also determined by dual labeling for the superoxide producing enzyme and tyrosine hydroxylase (TH), the rate-limiting enzyme in catecholamine biosynthesis. In the mNTS, NADPH oxidase subunits were present primarily in somatodendritic processes and astrocytes, some of which also contained TH, or were contacted by TH labeled axons, respectively. Immunogold quantification of NADPH oxidase subunit localization showed that p47 phox and gp91 phox were present on the surface membrane, as well as vesicular organelles characteristic of calcium storing smooth endoplasmic reticula in dendritic and astroglial processes. These results indicate that NADPH oxidase assembly and consequent superoxide formation are likely to occur near the plasmalemma, as well as on vesicular organelles associated with intracellular calcium storage within mNTS neurons and glia. Thus, NADPH oxidase-derived superoxide may participate in intracellular signaling pathways linked to calcium regulation in diverse mNTS cell types. Moreover, NADPH oxidase-derived superoxide in neurons and glia may directly or indirectly modulate catecholaminergic neuron activity in the mNTS.

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Molecular composition and regulation of the Nox family NAD(P)H oxidases

Cited by 275 publications

References 87 publications

Ionizing irradiation induces apoptotic damage of salivary gland acinar cells via NADPH oxidase 1-dependent superoxide generation

Ionizing irradiation induces apoptotic damage of salivary gland acinar cells via NADPH oxidase 1-dependent superoxide generation

Changes in the subcellular distribution of NADPH oxidase subunit p47phox in dendrites of rat dorsomedial nucleus tractus solitarius neurons in response to chronic administration of hypertensive agents

Subcellular localization of nicotinamide adenine dinucleotide phosphate oxidase subunits in neurons and astroglia of the rat medial nucleus tractus solitarius: Relationship with tyrosine hydroxylase immunoreactive neurons

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