Molecular comparison of mouse Thy-1 and its human homologue

Bonewald, Lynda F.; Wang, A. C.; Terry, Elaine; Zwerner, Robert K.; Balch, Charles M.; Ades, Edwin W.

doi:10.1016/0161-5890(82)90216-4

Cited by 7 publications

(2 citation statements)

References 11 publications

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“…Human Thy-1 (also designated p25) has been isolated from the human MOLT-3 lymphoblastoid T cell line and shown to be serologically related to mouse Thy-1.2 (Ades el nl., 1980). Human Thy-1 has the same molecular weight as its mouse counterpart (Bonewald et al, 1982) and shows similarities, though not identity, to its mouse homologue by peptide mapping and amino acid composition. Similar conclusions have Seen drawn using Thy-1 glycoprotein isolated from human brain and fibroblasts (Cotmore et a!., 1981).…”

Section: Introductionmentioning

confidence: 99%

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Biochemical Characterization of Human Thy‐1

Bonewald¹,

Ades

Tung³

et al. 1984

Int J Immunogenetics

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SUMMARY The human Thy‐1 homologue (p25) was characterized biochemically for amino acid composition, sequence and carbohydrate content. Two other forms of the human Thy‐1 molecule were detected and partially characterized. A 40,000 mol.wt. molecule (p40) is the dimer of p25 and its formation is increased by the presence of sodium dodecyl sulphate (SDS). The second form of 16,000 mol.wt. (p16) appears to be a cryptic or breakdown form of p25. Comparison of the amino acid compositions of p25, p40 and p16 isolated from MOLT‐3 cells, with that deduced from the nucleotide sequence of the gene coding for part of the putative T cell antigen receptor, also from MOLT‐3 cells, shows that the Thy‐1 homologue is distinct from, but evolutionary related to, one of the putative T cell antigen receptor polypeptide chains.

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Section: Introductionmentioning

confidence: 99%

“…A 40,000 mol.wt. molecule (p40) is the dimernof p25 and its Formation is increased by the Characterization of human Thy-1 285 of protein was performed as described previously (Bonewald et al, 1982). The purified protein was concentrated by lyophilization and then applied to a 12 cm x 0-5 cm Sephadex G-25 column in distilled water.…”

mentioning

confidence: 99%

Biochemical Characterization of Human Thy‐1

Bonewald¹,

Ades

Tung³

et al. 1984

Int J Immunogenetics

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show abstract

Chicken thymocyte‐specific antigen identified by monoclonal antibodies: ontogeny, tissue distribution and biochemical characterization

1984

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Two monoclonal antibodies, CT-1 (IgG1, kappa) and CT-1a (IgG3, kappa) were prepared against chicken thymocytes. The antigen identified by these antibodies was found to be a glycoprotein with a major polypeptide component having an apparent molecular weight of 63 000 and a minor polypeptide component of approximately 103 000. Immunoprecipitation and blocking experiments revealed that the two antibodies react with different antigenic determinants of the molecule. Ontogenic studies employing immunofluorescence failed to reveal the antigenic determinants on cells from the embryo or embryonic yolk sac on days 3 and 6 of incubation. The number of embryonic thymocytes bearing the molecules detected by these antibodies increased from 6% on day 12 to 54% on day 13; the frequency of thymocytes expressing this glycoprotein reached adult levels (greater than 90%) by the 15th day of embryonic age. In contrast, the CT-1 and CT-1a antibodies reacted with only 2-5% of blood and splenic cells and less than 0.05% of cells from bursa or bone marrow of young adult chickens. In the quail CT-1 reacted with cortical, but not medullary, thymocytes, while the CT-1a antibody was unreactive with quail thymocytes. The surface glycoproteins detected by these discriminating monoclonal antibodies may provide an important discriminating marker for thymic lymphocytes in the chicken and the quail.

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Expression of Thy-1 and effect of phosphatidylinositol-specific phospholipase C on primate and murine cell lines

Feng¹,

Wang²

1988

Cellular Immunology

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Molecular comparison of mouse Thy-1 and its human homologue

Cited by 7 publications

References 11 publications

Biochemical Characterization of Human Thy‐1

Biochemical Characterization of Human Thy‐1

Chicken thymocyte‐specific antigen identified by monoclonal antibodies: ontogeny, tissue distribution and biochemical characterization

Expression of Thy-1 and effect of phosphatidylinositol-specific phospholipase C on primate and murine cell lines

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