Molecular cloning, tissue expression, and chromosomal assignment of a novel gene encoding a subunit of the human signal-recognition particle

Abstract

Human cancers derived from breast, esophageal, or ovarian tissues frequently show allelic losses on chromosome band 17q25. Moreover, a locus responsible for hereditary focal nonepidermolytic palmoplantar keratoderma, a condition associated with esophageal cancer (TOC; tylosis with oesophageal cancer), has been mapped to the same band. During efforts to sequence, by shotgun methods, a 1-Mb target region that we had defined as the DNA segment harboring the putative tumor suppressor gene(s) involved in these events, we identified a novel cDNA. The full-length cDNA is 2495 bp long and is expressed predominantly in skeletal muscle, heart, kidney, and placenta. The predicted product, a 627-amino-acid protein, exhibited significant sequence homology to the canine 68-kd subunit of the signal recognition particle that has been implicated in the transport of secreted and membrane proteins to the endoplasmic reticulum for proper processing. We confirmed the location of this gene at chromosome 17q25.1 by radiationhybrid mapping and by fluorescence in situ hybridization.

Cooperative and competitive regulation of the astrocytic transcriptome by neurons and endothelial cells: Impact on astrocyte maturation

“…We also included some genes that are highly enriched/almost exclusively expressed by astrocytes (i.e., aquaporin 4 [ Aqp4 ], connexin 43 [ Gja1 ], glutamine synthetase [ Glul ], nuclear factor I A [ Nfia ], glutamate transporter 1 [ Slc1a2 ], and SRY‐box transcription factor 9 [ Sox9 ]). We selected histidyl‐tRNA synthetase 2 ( Hars2 ), S1 RNA binding domain 1 ( Srbd1 ), and signal recognition particle 68 ( Srp68 ) as controls for the qPCR since they have the lowest coefficient of variance between culture configurations and are required for basic cellular functions (Freist et al, 1999; Harada et al, 2001; Subramanian, 1983). The results of these analyses can be found in Table S4.…”

“…We chose some genes regulated by neurons but not endothelial cells and vice versa. We also included some genes that are highly en- and are required for basic cellular functions (Freist et al, 1999;Harada et al, 2001;Subramanian, 1983). The results of these analyses can be found in Table S4.…”

Familial tylosis

“…DNA/RNA Note: Abnormality in this area has been noted in breast and ovarian cancer (Nagai et al, 2000;Harada et al, 2001). Description: 42.5kb.…”