2000
DOI: 10.1016/s0304-4165(99)00213-5
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Molecular cloning, tissue distribution and sequence analysis of complete glucokinase cDNAs from gilthead seabream (Sparus aurata), rainbow trout (Oncorhynchus mykiss) and common carp (Cyprinus carpio)

Abstract: The enzyme glucokinase (GK) (EC 2.7.1.1) plays an important role in the control of glucose homeostasis. Qualitative and/or quantitative variations in GK enzyme have been postulated by previous studies to explain why dietary carbohydrate utilisation is lower in gilthead seabream (Sparus aurata) and rainbow trout (Oncorhynchus mykiss) than in common carp (Cyprinus carpio). In this study, we report the isolation and characterisation of a full-length cDNA coding for GK in these teleosts. Amino acid sequences deriv… Show more

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Cited by 63 publications
(52 citation statements)
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“…Fish are generally considered to possess poor control over blood glucose levels (44), and one explanation for this was thought to be the absence of inducible hepatic GK activity (17,22). However, GK enzyme activities are reported in several fish species (21,22,23,39,40) and are induced by feeding (13) and dietary carbohydrates (21,22,39).…”
mentioning
confidence: 99%
“…Fish are generally considered to possess poor control over blood glucose levels (44), and one explanation for this was thought to be the absence of inducible hepatic GK activity (17,22). However, GK enzyme activities are reported in several fish species (21,22,23,39,40) and are induced by feeding (13) and dietary carbohydrates (21,22,39).…”
mentioning
confidence: 99%
“…The initial reports of liver glucokinase deficiency were based largely on the electrophoretic separation of hexokinase isozymes (see Ureta, 1982). More recent investigations that used more sensitive approaches have often detected low levels of glucokinase in the livers of many of these species (e.g., Panserat et al, 2000a;Berradi et al, 2005;Hiskett et al, 2009), but confirm, at least in some species, that the levels of glucokinase in their liver are low. It has been argued that low levels of glucokinase in the liver of these species is unlikely to be due to inactivating mutations in the glucokinase gene, as these mutations would likely disrupt essential glucokinase function (i.e., regulating insulin secretion) in the pancreas (Cárdenas et al, 1998;Wang et al, 2013).…”
Section: Glucose Metabolism and Dietmentioning
confidence: 99%
“…La inmunoreactividad para GK se ha visto no solamente en neuronas, sino también en tanacitos (Polakof et al, 2009) cuyo potencial capacidad glucosensora ha sido descrita en el cerebro de roedores (Rodríguez et al, 2005). En oposición a estos datos obtenidos en la especie carnívora trucha arco iris, en el pez omnívoro carpa común (Cyprinus carpio) la expresión de GK no se ha detectado en el cerebro (Panserat et al, 2000). La actividad y expresión de GK se incrementa en el hipotálamo y el cerebro posterior de trucha arco iris, cuando estos tejidos se someten a incrementos en la concentración de glucosa, ya sea in vivo o in vitro (Polakof et al, 2007b y c).…”
Section: Componentes Del Sistema Glucosensor a Nivel Centralunclassified
“…Los promotores "upstream" desencadenan la expresión de GK en tejidos no hepáticos, incluido el páncreas y el cerebro (Iynedjian, 2009). En peces, estudios realizados en dorada (Sparus aurata) revelan la presencia de un promotor de GK en el hígado (Egea et al, 2007).La expresión génica de GK en el conjunto del cerebro ha sido estudiada en dos especies de peces como son la trucha arco iris (Panserat et al, 2000) y el pez zebra (González-Álvarez et al, 2009). Además, la actividad/expresión génica de GK se ha descrito en cinco áreas específicas del cerebro tales como el telencéfalo, cerebro posterior, cerebro medio, hipotálamo (Soengas et al, 2006) e hipófisis (Polakof, comunicación personal).…”
unclassified
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