Molecular cloning, sequence identification, and gene expression analysis of bovine ADCY2 gene

Abstract

Adenylyl cyclase 2 (ADCY2), a class B member of adenylyl cyclases, is important in accelerating phosphor-acidification as well as glycogen synthesis and breakdown. Given its distinct role in flesh tenderization after butchering, we cloned and sequenced the ADCY2 gene from Yanbian cattle and assessed its expression in bovine tissues. A 2947 bp nucleotide sequence representing the full-length cDNA of bovine ADCY2 gene was obtained by 5' and 3' remote analysis computations for gene expression. Analyses of the putative protein sequence showed that ADCY2 had high homology among species, except with the non-mammal Oreochromis niloticus. Gene structural domain analyses in humans and rats indicated that the ADCY2 protein had no flaw; only the transmembrane domain was reduced and the CYCc structure domain was shortened. Assessment of ADCY2 expression in bovine tissues by real-time PCR showed that the highest expression was in the testes, followed by the longissimus dorsi, tensor fasciae latae, and latissimus dorsi. These data will serve as a foundation for further insight into the cattle ADCY2 gene.

Systems-level differential gene expression analysis reveals new genetic variants of oral cancer

“…We have seen a highly significant association between SERPINB5 expression and oral carcinoma 29 . ADCY2 is a membrane-associated enzyme which converts adenosine-5′-triphosphate (ATP) into 3′,5′-adenosine monophosphate (cyclic AMP/cAMP) and pyrophosphate 30 involving in the regulation of cAMP synthesis 31 . This gene catalyzes the signaling molecule cAMP through G-protein beta as well as gamma subunit signaling 32,33 .…”

Suxiao Jiuxin Pill protects cardiomyocytes against mitochondrial injury and alters gene expression during ischemic injury

“…In the present study, the increased levels of Adcy2, 3 and 8 mRNA in the ischemic injury model were significantly attenuated by SX treatment, and the levels of Adcy1 and 6 mRNA were significantly decreased following SX treatment. Adcy proteins (encoded by the Adcy gene) catalyze the conversion of ATP to cyclic adenosine monophosphate (cAMP) ( 35 ). Adcys are activated or inhibited by guanine nucleotide binding proteins (G proteins), which are coupled to membrane receptors that respond to hormonal stimulations, among others ( 35 ).…”

“…Adcy proteins (encoded by the Adcy gene) catalyze the conversion of ATP to cyclic adenosine monophosphate (cAMP) ( 35 ). Adcys are activated or inhibited by guanine nucleotide binding proteins (G proteins), which are coupled to membrane receptors that respond to hormonal stimulations, among others ( 35 ). cAMP, as a second messenger, serves as a regulatory signal through its interactions with cAMP-binding proteins, including protein kinase A, ion channels, transcription factors and enzymes ( 36 , 37 ).…”

“…ADCY2 protein, encoded by the Adcy2 gene, accelerates phosphor-acidification and the synthesis and breakdown of glycogen ( 35 ). Abnormal ADCY2 protein has been implicated in chronic obstructive pulmonary disease and bipolar disorder ( 38 , 39 ).…”

Protective potential of miR-146a-5p and its underlying molecular mechanism in diverse cancers: a comprehensive meta-analysis and bioinformatics analysis

“…Moreover, PRKACB might be a promising target in cancer treatment by increasing drugs responsiveness to tumors [108,109]. Adenylate cyclase 2 (ADCY2), a gene related to the production of cAMP, is greatly implied in acceleration of phosphor-acidification and metabolic processes of glycogen [110,111]. Duerr et al found that ADCY2 expression was obviously elevated in pancreatic neuroendocrine malignant tumors, possibly linked with tumor invasiveness [112].…”