Molecular Cloning, Refined Physical Map and Heterogeneity of Methylation Sites of Papilloma Virus Type 1a DNA

Danos, O; Katinka, Michaël; Yaniv, Moshe

doi:10.1111/j.1432-1033.1980.tb04815.x

Cited by 58 publications

(35 citation statements)

References 24 publications

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“…Methylation is not incompatible with extrachromosomal maintenance per se, since there are some viral DNAs which contain methylated sites when maintained in an extrachromosomal state (4,18,33,35,49). For example, certain sites were methylated in Shope papillomavirus (49), Epstein-Barr virus (18,33,35), and human papillomavirus type la (4).…”

Section: Discussionmentioning

confidence: 99%

In vitro methylation of bovine papillomavirus alters its ability to transform mouse cells.

Christy¹,

Scangos²

1986

Mol. Cell. Biol.

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Bovine papillomavirus (BPV) was methylated in vitro at either the 29 HpaII sites, the 27 HhaI sites, or both. Methylation of the HpaII sites reduced transformation by the virus two-to sixfold, while methylation at HhaI sites increased transformation two-to fourfold. DNA methylated at both HpaII and HhaI sites did not differ detectably from unmethylated DNA in its efficiency of transformation. These results indicate that specific methylation sites, rather than the absolute level of methylated cytosine residues, are important in determining the effects on transformation and that the negative effects of methylation at some sites can be compensated for by methylation at other sites. BPV molecules in cells transformed by methylated BPV DNA contained little or no methylation, indicating that the pattern of methylation was not faithfully retained in these extrachromosomally replicating molecules. Methylation at the HpaII sites (but not the HhaI sites) in the cloned BPV plasmid or in pBR322 also inhibited transformation of the plasmids into Escherichia coli HB101 cells.

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Section: Discussionmentioning

confidence: 99%

In vitro methylation of bovine papillomavirus alters its ability to transform mouse cells.

Christy¹,

Scangos²

1986

Mol. Cell. Biol.

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“…DNA methylation in HPV was first described nearly 30 years ago (Danos et al, 1980) and its role in carcinogenesis has been studied subsequently. HPV16 and HPV18 are the most thoroughly analyzed virus types, as they represent the high-risk types of HPV.…”

Section: Human Papilloma Virusmentioning

confidence: 99%

Viral epigenomes in human tumorigenesis

Fernández

Esteller

2010

Oncogene

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Viruses are associated with 15-20% of human cancers worldwide. In the last century, many studies were directed towards elucidating the molecular mechanisms and genetic alterations by which viruses cause cancer. The importance of epigenetics in the regulation of gene expression has prompted the investigation of virus and host interactions not only at the genetic level but also at the epigenetic level. In this study, we summarize the published epigenetic information relating to the genomes of viruses directly or indirectly associated with the establishment of tumorigenic processes. We also review aspects such as viral replication and latency associated with epigenetic changes and summarize what is known about epigenetic alterations in host genomes and the implications of these for the tumoral process. The advances made in characterizing epigenetic features in cancer-causing viruses have improved our understanding of their functional mechanisms. Knowledge of the epigenetic changes that occur in the genome of these viruses should provide us with markers for following cancer progression, as well as new tools for cancer therapy.

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“…Reports more than 20 years ago on HPV-1 and the cottontail rabbit papillomavirus suggested that papillomaviruses are targeted by cellular DNA methylation, although limited analytical power, namely methylation-sensitive restriction enzymes, was accepted as the state of the art of that time (9,13,40,47). Research on the methylation of HPVs was extended recently to the high-risk HPV-16 (2,22) by bisulfite modification and DNA sequencing.…”

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confidence: 99%

Conserved Methylation Patterns of Human Papillomavirus Type 16 DNA in Asymptomatic Infection and Cervical Neoplasia

Kalantari

Calleja-Macías

Tewari

et al. 2004

J Virol

165

181

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Molecular Cloning, Refined Physical Map and Heterogeneity of Methylation Sites of Papilloma Virus Type 1a DNA

Cited by 58 publications

References 24 publications

In vitro methylation of bovine papillomavirus alters its ability to transform mouse cells.

In vitro methylation of bovine papillomavirus alters its ability to transform mouse cells.

Viral epigenomes in human tumorigenesis

Conserved Methylation Patterns of Human Papillomavirus Type 16 DNA in Asymptomatic Infection and Cervical Neoplasia

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