Molecular cloning ofTvDAO1, a gene encoding aD-amino acid oxidase fromTrigonopsis variabilis and its expression inSaccharomyces cerevisiae andKluyveromyces lactis

González, Francisco; Montes, Javier del Pino; Martín, Fernando A.; López, M.C.; Fermiñán, Encarnación; Catalán, Jacinto; Galán, Miguel Á.; Domínguez, Ángel

doi:10.1002/(sici)1097-0061(199712)13:15<1399::aid-yea187>3.0.co;2-7

Cited by 35 publications

(4 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…TvDAAO was expressed in Saccharomyces cerevisiae, Kluyveromyces lactis [86], and S. pombe [97] yeast, but the yield achieved was no higher than for E. coli cells [90]. It was probably the result of low level synthesis of FAD, which is obliga tory for holoenzyme production.…”

Section: Daao Gene Cloning and Expressionmentioning

confidence: 99%

D-amino acid oxidase: structure, catalytic mechanism, and practical application

Тишков

Khoronenkova

2005

Biochemistry (Moscow)

View full text Add to dashboard Cite

D-Amino acid oxidase (DAAO) is a FAD-dependent enzyme that plays an important role in microbial metabolism, utilization of endogenous D-amino acids, regulation of the nervous system, and aging in mammals. DAAO from yeasts Rhodotorula gracilis and Trigonopsis variabilis are used to convert cephalosporin C into 7-aminocephalosporanic acid, the precursor of other semi-synthetic cephalosporins. This review summarizes the recent data on the enzyme localization, physiological role, gene cloning and expression, and the studies on the enzyme structure, stability, catalytic mechanism, and practical applications.

show abstract

Section: Daao Gene Cloning and Expressionmentioning

confidence: 99%

D-amino acid oxidase: structure, catalytic mechanism, and practical application

Тишков

Khoronenkova

2005

Biochemistry (Moscow)

View full text Add to dashboard Cite

show abstract

“…Gonzalez and colleagues took advantage of the resembling lac-gal -regulon in K. lactis and applied the S. cerevisiae promoter P GAL1 to express Trigonopsis variabilis D-aminoacid oxidase ( DAO1 ) in K. lactis [97]. …”

Section: Introductionmentioning

confidence: 99%

Carbon source dependent promoters in yeasts

et al. 2014

View full text Add to dashboard Cite

Budding yeasts are important expression hosts for the production of recombinant proteins.The choice of the right promoter is a crucial point for efficient gene expression, as most regulations take place at the transcriptional level. A wide and constantly increasing range of inducible, derepressed and constitutive promoters have been applied for gene expression in yeasts in the past; their different behaviours were a reflection of the different needs of individual processes.Within this review we summarize the majority of the large available set of carbon source dependent promoters for protein expression in yeasts, either induced or derepressed by the particular carbon source provided. We examined the most common derepressed promoters for Saccharomyces cerevisiae and other yeasts, and described carbon source inducible promoters and promoters induced by non-sugar carbon sources. A special focus is given to promoters that are activated as soon as glucose is depleted, since such promoters can be very effective and offer an uncomplicated and scalable cultivation procedure.

show abstract

“…For this purpose, genes are searched for in sequenced genomes, the number of which is constantly increasing. The genes of D-amino acid oxidases from yeast and fungi were cloned, and the properties of the enzymes were studied from only 7 sources: Fusarium solani (FsoDAAO) [ 12 ], Trigonopsis variabilis (TvaDAAO) [ 13 ], Rhodosporidium toruloides (formerly Rhodotorula gracilis) (RtoDAAO) [ 14 ], Pichia pastoris (PpaDAAO) [ 15 ], Candida boidinii (CboDAAO) [ 16 ], Rasamsonia emersonii strain YA (RemDAAO) [ 17 ], and Ogataea parapolymorpha DL-1 [ 18 ]. In the latter case, the genes of five different DAAOs (OpaDAAO1 – OpaDAAO5) and one D-aspartate oxidase (DASPO) were identified, cloned, and expressed in E. coli.…”

Section: Introductionmentioning

confidence: 99%

“…С этой целью проводят поиск генов в секвенированных геномах, число которых постоянно возрастает. У оксидаз D-аминокислот из дрожжей и грибов клонированы гены и изучены свойства ферментов всего из 7 источников -Fusarium solani (FsoDAAO) [12], Trigonopsis variabilis (TvaDAAO) [13], Rhodosporidium toruloides (ранее Rhodotorula gracilis) (RtoDAAO) [14], Pichia pastoris (PpaDAAO) [15], Candida boidinii (CboDAAO) [16], Rasamsonia emersonii штамм YA (RemDAAO) [17] и Ogataea parapolymorpha DL-1 [18]. Причем в последнем случае идентифицировали, клонировали и экспрессировали в E. coli гены пяти разных DAAO (OpaDAAO1 -OpaDAAO5) и одной D-аспартатоксидазы (DASPO).…”

Section: Introductionunclassified

Bioinformatics-Structural Approach to the Search for New D-Amino Acid Oxidases

et al. 2023

View full text Add to dashboard Cite

D-amino acid oxidase (DAAO, EC 1.2.1.2) plays an important role in the functioning of prokaryotes as well as of lower (yeast and fungi) and higher eukaryotes (mammals). DAAO genes have not yet been found in archaean genomes. D-amino acid oxidase is increasingly used in various fields, which requires the development of new variants of the enzyme with specific properties. However, even within one related group (bacteria, yeasts and fungi, mammals), DAAOs show very low homology between amino acid sequences. In particular, this fact is clearly observed in the case of DAAO from bacteria. The high variability in the primary structures of DAAO severely limits the search for new enzymes in known genomes. As a result, many (if not most) DAAO genes remain either unannotated or incorrectly annotated. We propose an approach that uses bioinformatic methods in combination with general 3D structure and active center structure analysis to confirm that the gene found encodes D-amino acid oxidase and to predict the possible type of its substrate specificity. Using a homology search, we obtained a set of candidate sequences, modelled the tertiary structure of the selected enzymes, and compared them with experimental and model structures of known DAAOs. The effectiveness of the proposed approach for discrimination of DAAOs and glycine oxidases is shown. Using this approach, new DAAO genes were found in the genomes of six strains of extremophilic bacteria, and for the first time in the world, one gene was identified in the genome of halophilic archaea. Preliminary experiments confirmed the predicted specificity of DAAO from Natronosporangium hydrolyticum ACPA39 with D-Leu and D-Phe.

show abstract

Molecular cloning ofTvDAO1, a gene encoding aD-amino acid oxidase fromTrigonopsis variabilis and its expression inSaccharomyces cerevisiae andKluyveromyces lactis

Cited by 35 publications

References 41 publications

D-amino acid oxidase: structure, catalytic mechanism, and practical application

D-amino acid oxidase: structure, catalytic mechanism, and practical application

Carbon source dependent promoters in yeasts

Bioinformatics-Structural Approach to the Search for New D-Amino Acid Oxidases

Contact Info

Product

Resources

About