Molecular cloning of vitellogenin gene promoters and in vitro and in vivo transcription profiles following estradiol-17β administration in the cutthroat trout

Oogenesis and Egg Quality in Finfish: Yolk Formation and Other Factors Influencing Female Fertility

“…Vitellogenin receptors cluster into clathrin coated vesicles that are endocytosed and vitellogenins are processed into yolk proteins for storage in yolk granules located throughout the ooplasm (Yolk Deposition). The figure summarizes data from: [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22]25,[28][29][30][31]134].…”

“…The transcriptional potential of different vitellogenin genes seems to be determined by the number of ERE or ERE-like sequences within the promoter region of each, as well as numbers and/or types of additional transcription factor binding sites, such as GATA. These promoter components and structures differ across fish species, as well as among discrete vitellogenin gene subtypes within a species [11,139,140]. In teleosts, there are several forms of estrogen receptor [141].…”

“…In teleosts, there are several forms of estrogen receptor [141]. The ERα seems to play an important role in the transcription of vitellogenin genes, because expression of hepatic ERα mRNA is up-regulated by E2 stimulation [11,12,138,142]. However, ERα is not the only ER subtype to regulate vitellogenin gene expression; ERβ subtypes also have been suggested as essential factors to promote vitellogenin synthesis [143,144].…”

Hepatic estrogen-responsive genes relating to oogenesis in cutthroat trout (Oncorhynchus clarki): The transcriptional induction in primary cultured hepatocytes and the in vitro promoter transactivation in responses to estradiol-17β

“…Meanwhile, highly evolved acanthomorph species are likely to express three Vtg subtypes, VtgAa (previously termed VtgA), VtgAb (previously termed VtgB), and VtgC (Hiramatsu et al, 2005). Salmonids, on the other hand, express multiple copies of salmonid-type A Vtg (VtgAs) alongside a VtgC orthologue (Buisine et al, 2002;Mushirobira et al, 2018;Trichet et al, 2000).…”

“…Because each of these studies used different species and/or did not consider the multiplicity of chg, vtg and esr, it has been difficult to obtain an integrated view of transcriptional regulation of these genes in response to E2 stimulation; it is therefore needed to perform such a study in a single species and include all subtypes of the genes of interest. Recently, the two promoters of vtgAs (1 and 2) and one promoter of vtgC for cutthroat trout (Oncorhynchus clarki) have been cloned and analyzed (Mushirobira et al, 2018).…”