1980
DOI: 10.1007/bf00425449
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Molecular cloning of the T4 genome; Organization and expression of the frd-DNA ligase region

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1981
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Cited by 47 publications
(27 citation statements)
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“…The thin line to the right of the EcoRI site (RI) of the polylinker designates the right arm of the lambda genome, including a functional attachment site (att), the phage 21 immunity region substitution (mm21) and the nin5 deletion. This right arm, although directly donated by XTL25, was originally derived from XNM616 (29). The sequence of the polylinker-RNaseIIl region is presented in Fig.…”
Section: Methodsmentioning
confidence: 99%
“…The thin line to the right of the EcoRI site (RI) of the polylinker designates the right arm of the lambda genome, including a functional attachment site (att), the phage 21 immunity region substitution (mm21) and the nin5 deletion. This right arm, although directly donated by XTL25, was originally derived from XNM616 (29). The sequence of the polylinker-RNaseIIl region is presented in Fig.…”
Section: Methodsmentioning
confidence: 99%
“…In turn, the onset of phage DNA replication, which occurs at about 5 min after infection, is dependent on the turning on of deoxyribonucleotide synthesis (13,33,45,51). Accordingly, workers in our laboratory are investigating the control of expression of the T4 nrdA and nrdB genes, which encode the a2 and 2 subunits, respectively, of ribonucleotide reductase (4,5,16,36,39,43,46). nrdC, which encodes phage thioredoxin, is unlikely to be limiting in the initiation of ribonucleotide reductase synthesis (7,46).…”
mentioning
confidence: 99%
“…1) containing the 3' end of nrdA, exon I, and the 5' half of the intron of the nrdB gene was excised from Xtd652. The 3' half of the intron, exon II of the nrdB gene, the denA gene and a 5' segment of gene 63 were contained in the 1.7-kb EcoRI fragment isolated from T4D deoxycytidylate DNA (14). These two fragments were inserted into pTZ19R to obtain pMTnrdB.…”
mentioning
confidence: 99%
“…E. coli N4830 and the expression plasmids PPL-P and pKK223-3 were from Pharmacia LKB Biotechnology. Xtd30 (a hybrid of phages X and T4D) and Xtd652 (a hybrid of phages X and T4D) were described by Mileham et al (14). Materials and chemicals were as previously reported (23,24).…”
mentioning
confidence: 99%
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