Molecular cloning of the second major allergen, <i>Cry j</i> II, from Japanese cedar pollen

Namba, Motoshi; Kurose, Mayumi; Torigoe, Kakuji; Hino, Ken‐ichi; Taniguchi, Yoshifumi; Fukuda, Shigeharu; Usui, Mitsuko; Kurimoto, Masashi

doi:10.1016/0014-5793(94)01022-6

Cited by 69 publications

(46 citation statements)

References 18 publications

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“…Japanese cedar (Cryptomeria japonica) pollen is a major cause of pollinosis that elicits allergic disorders such as rhinitis and conjunctivitis in Japan (9). Two major allergens, designated Cry j I and Cry j II, were isolated from the pollen (9)(10)(11)(12)(13), and multiple domains of T cell epitope for humans and mice were identified from them (14)(15)(16). It has been reported that oral feeding to mice of a chemically synthesized major T cell epitope peptide of Cry j II reduces levels of Cry j II-specific IgE and IgG antibody responses via a decrease in the production of allergy-associated IL-4 in mice (15).…”

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confidence: 99%

A rice-based edible vaccine expressing multiple T cell epitopes induces oral tolerance for inhibition of Th2-mediated IgE responses

Takagi

Hiroi

Yang

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

204

137

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Peptide immunotherapy using multiple predominant allergen-specific T cell epitopes is a safe and promising strategy for the control of type I allergy. In this study, we developed transgenic rice plants expressing mouse dominant T cell epitope peptides of Cry j I and Cry j II allergens of Japanese cedar pollen as a fusion protein with the soybean seed storage protein glycinin. Under the control of the rice seed storage protein glutelin GluB-1 promoter, the fusion protein was specifically expressed and accumulated in seeds at a level of 0.5% of the total seed protein. Oral feeding to mice of transgenic rice seeds expressing the T cell epitope peptides of Cry j I and Cry j II before systemic challenge with total protein of cedar pollen inhibited the development of allergen-specific serum IgE and IgG antibody and CD4 ؉ T cell proliferative responses. The levels of allergen-specific CD4 ؉ T cell-derived allergy-associated T helper 2 cytokine production of IL-4, IL-5, and IL-13 and histamine release in serum were significantly decreased. Moreover, the development of pollen-induced clinical symptoms was inhibited in our experimental sneezing mouse model. These results indicate the potential of transgenic rice seeds in production and mucosal delivery of allergen-specific T cell epitope peptides for the induction of oral tolerance to pollen allergens.Japanese cedar pollinosis ͉ peptide immunotherapy ͉ seed-specific expression

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confidence: 99%

A rice-based edible vaccine expressing multiple T cell epitopes induces oral tolerance for inhibition of Th2-mediated IgE responses

Takagi

Hiroi

Yang

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

204

137

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“…Recently, we established an effective procedure for the introduction of DNA into mature zygotic embryos of three species of Japanese conifer, including C. japonica . We have also isolated genes for various allergens , Namba et al 1994, Futamura et al 2002 and for so-called "MADS box" proteins (Fukui et al 2001) from C. japonica. Therefore, in the near future, the present system for the regeneration of C. japonica should allow the genetic engineering of transgenic C. japonica with allergen-free pollen grains.…”

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confidence: 99%

Phytosulfokine Stimulates Somatic Embryogenesis in Cryptomeria japonica

Igasaki

Akashi

Ujino‐Ihara

et al. 2003

Plant and Cell Physiology

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; Phytosulfokine (PSK), which has been identified as a plant growth factor, had a dramatic stimulatory effect on the formation of somatic embryos of sugi (Cryptomeria japonica) in the presence of polyethylene glycol. The resultant somatic embryos germinated with synchronous sprouting of cotyledons, hypocotyls and roots, and most of the seedlings grew normally. A cDNA clone for the precursor to the PSK peptide of C. japonica was identified in an expressed sequence tags database. Our results support the existence of a PSK signaling pathway in C. japonica.

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“…From the analysis of five Cry j 2 cDNA clones [61], a complete sequence of 388 amino acids was deduced, containing one glycosylation site in the C-terminal end. The mature protein had an estimated molecular weight of 42,000 D and showed no homology with Cry j 1.…”

Section: Molecular Cloningmentioning

confidence: 99%

Cupressaceae Pollinosis: Identification, Purification and Cloning of Relevant Allergens

Felice

Barletta

Tinghino

et al. 2001

Int Arch Allergy Immunol

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Allergy to Cupressaceae pollen is a worldwide pollinosis caused by several species. Pollen extracts prepared from allergenic species belonging to this family are characterised by low protein and high carbohydrate content. The allergenic components represented in the pollen extracts from different species of the Cupressaceae family show high levels of cross-reactivity when probed with human IgE from allergic subjects and share a number of common epitopes also identified by polyclonal rabbit antisera and monoclonal antibodies. A close relationship has also been described with the Taxodiaceae and Podocarpaceae families. Although both proteic and carbohydrate epitopes appear to be involved in IgE recognition and allergenic cross-reactivity, a large portion of the IgE reactivity of Cupressaceae-allergic patients seems to be associated with sugar moieties present on the relevant allergenic molecules. From this point of view, Cupressaceae/Taxodiaceae allergens constitute a particularly useful model to study IgE cross-reactivity, as they have been shown to display different levels of homology. Moreover, the availability of the purified allergens, together with their recombinant counterparts, may shed light on the actual role played by carbohydrate in allergic sensitisation, IgE recognition and allergenic cross-reactivity.

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Molecular cloning of the second major allergen, Cry j II, from Japanese cedar pollen

Cited by 69 publications

References 18 publications

A rice-based edible vaccine expressing multiple T cell epitopes induces oral tolerance for inhibition of Th2-mediated IgE responses

A rice-based edible vaccine expressing multiple T cell epitopes induces oral tolerance for inhibition of Th2-mediated IgE responses

Phytosulfokine Stimulates Somatic Embryogenesis in Cryptomeria japonica

Cupressaceae Pollinosis: Identification, Purification and Cloning of Relevant Allergens

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