Molecular cloning of the ribosomal P-proteins MgP1, MgP2, MgP0, and superoxide dismutase (SOD) in the mussel Mytilus galloprovincialis and analysis of MgP0 at stress conditions

Kolaiti, Regina-Maria; Lucas, Jared M.; Kouyanou-Koutsoukou, Sophia

doi:10.1016/j.gene.2008.10.024

Cited by 13 publications

(6 citation statements)

References 51 publications

(60 reference statements)

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“…For MgeCuZnSOD, no signal peptide was found, indicating that it belongs to a member of the intracellular CuZnSOD family. Alignment of MgeCuZnSOD showed that only 63 identical amino acid residues with previous reported intracellular CuZnSOD from M. galloprovincialis [25]. However, MgeCuZnSOD encoded a polypeptide of 211 amino acids, which was much longer than previous reported intracellular CuZnSODs from mollusks.…”

Section: Discussionmentioning

confidence: 52%

“…To our knowledge, there is no report about MnSOD gene in mussels including M. galloprovincialis. Although a CuZnSOD gene has been reported in M. galloprovincialis [25], its transcriptional response against bacterial infection has not been investigated. Therefore, the objective of this study was to isolate and characterize SOD genes in order to understand the transcriptional response of these genes against bacterial challenge in M. galloprovincialis.…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of a manganese superoxide dismutase and copper/zinc superoxide dismutase from the mussel Mytilus galloprovincialis

Wang

Yuan

et al. 2013

Fish & Shellfish Immunology

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Section: Discussionmentioning

confidence: 52%

Section: Introductionmentioning

confidence: 99%

Molecular characterization of a manganese superoxide dismutase and copper/zinc superoxide dismutase from the mussel Mytilus galloprovincialis

Wang

Yuan

et al. 2013

Fish & Shellfish Immunology

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“…The ribosomal stalk is involved in the control of protein synthesis by affecting the expression of specific mRNAs and the phosphorylation of P-proteins by CK2 kinase seems to control the ribosomal activity [42]. M. galloprovincialis is used as an indicator of marine pollution and the ribosomal MgP0 protein is overexpressed in response to environmental stress [43]. As mentioned above, C. capitata and M. galloprovincialis seem to be ideal models for the study of cellular stress and gene expression but also for the analysis of human diseases [44], since neoplasia is also detected in mussels as a response to marine pollution.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Protein kinase CK2 from two higher eukaryotes of economical importance, the mussel Mytilus galloprovincialis and the medfly Ceratitis capitata

Kouyanou-Koutsoukou

Baier²,

Kolaitis³

et al. 2012

Open Life Sciences

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AbstractProtein kinase CK2 is a highly conserved Ser/Thr protein kinase involved in cell cycle control, transcription, signal transduction and cell proliferation. It is upregulated in several diseases and by oxidative stress. CK2 is generally composed of two catalytic subunits and two regulatory subunits and utilizes either ATP or GTP as a phosphate donor. CK2 was isolated from the sea mussel Mytilus galloprovincialis, a biomarker of marine pollution, and the Mediterranean fly Ceratitis capitata, an insect capable of wreaking extensive damage to a wide range of fruit crops with great economical importance. The catalytic CK2α and regulatory CK2β subunits of M. galloprovincialis and C. capitata show similar properties. The mussel and fly catalytic subunits and holoenzymes were capable of phosphorylating the recombinant ribosomal stalk P1 protein, implying functional conservation. They also demonstrate the characteristics of a typical CK2: use of ATP and GTP as phosphate donors, inhibition by known modulators of CK2 activity (like benzotriazole derivatives and heparin), and stimulation by polycations. Both organisms seem to be ideal models for the analysis of CK2 in the control of gene expression in response to cellular stress.

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“…In the case of M . galloprovincialis , only a limited number of genes involved in the antioxidant system, such as GST-pi and superoxide dismutase (SOD), have been investigated (Hoarau et al, 2006;Kolaiti et al, 2009 …”

Section: Introductionmentioning

confidence: 99%

Molecular cloning, characterization and expression profiles of thioredoxin 1 and thioredoxin 2 genes in Mytilus galloprovincialis

Wang

Ning

Pei³

et al. 2013

Chin. J. Ocean. Limnol.

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Thioredoxin (Trx) proteins are involved in many biological processes especially the regulation of cellular redox homeostasis. In this study, two Trx cDNAs were cloned from the mussel Mytilus galloprovincialis using rapid amplifi cation of cDNA ends-polymerase chain reaction (RACE-PCR). The two cDNAs were named MgTrx1 and MgTrx2, respectively. The open reading frames of MgTrx1 and MgTrx2 were 318 and 507 base pairs (bp) and they encoded proteins of 105 and 168 amino acids with estimated molecular masses of 11.45 and 18.93 kDa, respectively. Sequence analysis revealed that both proteins possessed the conserved active site dithiol motif Cys-Gly-Pro-Cys. In addition, MgTrx2 also possessed a putative mitochondrial targeting signal suggesting that it is located in the mitochondria. Quantitative real-time polymerase chain reaction (qPCR) revealed that both MgTrx1 and MgTrx2 were constitutively expressed in all tissues examined. The MgTrx1 transcript was most abundant in hemocytes and gills, whereas the MgTrx2 transcript was most abundant in gonad, hepatopancreas, gill and hemocytes. Following Vibrio anguillarum challenge, the expression of MgTrx1 was up-regulated and reached its peak, at a value 10-fold the initial value, at 24 h. Subsequently, expression returned back to the original level. In contrast, the expression level of MgTrx2 was down-regulated following bacterial stimulation, with one fi fth of the control level evident at 12 h post challenge. These results suggest that MgTrx1 and MgTrx2 may play important roles in the response of M . galloprovincialis to bacterial challenge.

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Molecular cloning of the ribosomal P-proteins MgP1, MgP2, MgP0, and superoxide dismutase (SOD) in the mussel Mytilus galloprovincialis and analysis of MgP0 at stress conditions

Cited by 13 publications

References 51 publications

Molecular characterization of a manganese superoxide dismutase and copper/zinc superoxide dismutase from the mussel Mytilus galloprovincialis

Molecular characterization of a manganese superoxide dismutase and copper/zinc superoxide dismutase from the mussel Mytilus galloprovincialis

Protein kinase CK2 from two higher eukaryotes of economical importance, the mussel Mytilus galloprovincialis and the medfly Ceratitis capitata

Molecular cloning, characterization and expression profiles of thioredoxin 1 and thioredoxin 2 genes in Mytilus galloprovincialis

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