Molecular cloning of the gene for the allatostatin family of neuropeptides from the cockroach Diploptera punctata.

Donly, B. Cameron; Ding, Qi; Tobe, Stephen S.; Bendena, William G.

doi:10.1073/pnas.90.19.8807

Cited by 148 publications

(60 citation statements)

References 20 publications

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“…In insects, it has been found that neuropeptides such as diapause hormone and allatostatin are first synthesized as precursors [22,23]. These precursors often produce multiple peptides with distinct biological activities [24].…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning and characterization of cDNA for insect biogenic peptide, growth‐blocking peptide

et al. 1995

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Growth-blocking peptide (GBP) is an insect biogenic peptide that prevents the onset of metamorphosis from larva to pupa. A cDNA coding for GBP is described. Mixed oligonucleotides derived from a GBP peptide sequence were used to generate amplified DNA by the polymerase chain reaction (PCR). Based on the sequence of the amplified DNA, a 41 bases oligonucleotide was designed for screening a cDNA library which was constructed from the armyworm Pseudaletia separata larvae parasitized with the parasitic wasp Cotesia kariyai. The cloned cDNA for GBP was 809 base pairs in length. An open reading frame of 429 base pairs encodes a pre-pro-peptide of 143 amino acid residues in which GBP is localized at the C-terminal region, and other three peptides including a putative signal peptide and appropriate processing sites for endoproteolytic cleavage precede the GBP sequence. Northern blot analyses demonstrate the presence of a 800-base mRNA transcript in fat body and 2.5-kilobase transcript in brain and nerve cord, suggesting the possibility that the transcription of GBP gene is regulated in a tissue-dependent manner. This interpretation was supported by isolating a GBP cDNA fragment from cDNA pool of brain-nerve cords. GBP mRNA is constantly expressed in both parasitized and non-parasitized last instar larvae and there is no difference in the levels of the mRNA between both larvae, thus indicating that parasitism may effect on translational or posttranslational level to elevate plasma GBP concentration.

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Section: Discussionmentioning

confidence: 99%

Molecular cloning and characterization of cDNA for insect biogenic peptide, growth‐blocking peptide

et al. 1995

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“…Thirteen members of the FGLa/AST family, expressed as a prepropeptide, were first identified in D. punctata (AST 1-13) (Donly et al 1993). Since then, they have been found in Orthoptera, Dictyoptera, Isoptera, Lepidoptera, Diptera, and Hymenoptera (reviewed by Stay and Tobe 2007).…”

Section: Allatoregulatory Peptidesmentioning

confidence: 99%

Peptidergic control of food intake and digestion in insects¹This review is part of a virtual symposium on recent advances in understanding a variety of complex regulatory processes in insect physiology and endocrinology, including development, metabolism, cold hardiness, food intake and digestion, and diuresis, through the use of omics technologies in the postgenomic era.

et al. 2012

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Like all heterotrophic organisms, insects require a strict control of food intake and efficient digestion of food into nutrients to maintain homeostasis and to fulfill physiological tasks. Feeding and digestion are steered by both external and internal signals that are transduced by a multitude of regulatory factors, delivered either by neurons innervating the gut or mouthparts, or by midgut endocrine cells. The present review gives an overview of peptide regulators known to control feeding and digestion in insects. We describe the discovery and functional role in these processes for insect allatoregulatory peptides, diuretic hormones, FMRFamide-related peptides, (short) neuropeptide F, proctolin, saliva production stimulating peptides, kinins, and tachykinins. These peptides control either gut myoactivity, food intake, and (or) release of digestive enzymes. Some peptides exert their action at multiple levels, possibly having a biological function that depends on their site of delivery. Many regulatory peptides have been physically extracted from different insect species. However, multiple peptidomics, proteomics, transcriptomics, and genome sequencing projects have led to increased discovery and prediction of peptide (precursor) and receptor sequences. In combination with physiological experiments, these large-scale projects have already led to important steps forward in unraveling the physiology of feeding and digestion in insects.Key words: insect, midgut, digestion, neuropeptides, stomatogastric nervous system. Résumé : Comme tous les organismes hétérotrophes, les insectes requièrent un contrôle strict de leur ingestion de nourriture et la digestion de leur nourriture en nutriments afin de maintenir leur homéostasie et de compléter leurs tâches physiologiques. L'alimentation et la digestion sont gouvernées par des signaux tant externes qu'internes qui sont transmis par un multitude de facteurs régulateurs, livrés ou bien par les neurones qui innervent le tube digestif ou les pièces buccales ou alors par les cellules endocrines du tube digestif moyen. Notre revue fournit une synthèse des régulateurs peptidiques connus pour contrôler l'alimentation et la digestion chez les insectes. Nous décrivons la découverte et le rôle fonctionnel de ces processus dans le cas des peptides allatorégulateurs, des hormones diurétiques, des peptides apparentés aux amides FMRF, du neuropeptide F (court), de la proctoline, des peptides stimulateurs de la production de salive, des kinines et des tachykinines des insectes. Ces peptides contrôlent la myoactivité du tube digestif, l'ingestion de nourriture et(ou) la libération des enzymes digestives. Certains peptides agissent à de multiples niveaux, possédant une fonction biologique qui dépend de leur site d'action. Plusieurs peptides régulateurs ont été extraits physiquement de différentes espèces d'insectes. Cependant, plusieurs projets de peptidomique, de protéomique, de transcriptomique et de génomique ont mené à de nombreuses découvertes supplémentaires et des prédict...

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“…Peptides that are C-terminally-amidated require the amidation for biological activity (Pratt et al 1991a). The precursor also contains acidic domains that effectively neutralize the basic charge contribution of the peptides and their processing sites (Donly et al 1993;Ding et al 1995). The D. punctata FGLamide-AST precursor served as a prototype for all FGLa/AST precursors.…”

Section: The Fglamide-related-ast Familymentioning

confidence: 99%

“…This C-terminal core sequence is required for rapid and reversible inhibition of JH biosynthesis (Pratt et al 1991b;Stay et al 1991), but JH inhibition appears restricted to cockroaches, crickets, locusts, and termites (Stay and Tobe 2007;Clark et al 2008). The D. punctata AST gene was the first to be sequenced and revealed that a single open reading frame encoded a preproAST polypeptide precursor (Donly et al 1993). The D. punctata precursor contained sequences for 13 ASTs, each having the conserved pentapeptide C-terminus and a unique N-terminal sequence.…”

Section: The Fglamide-related-ast Familymentioning

confidence: 99%

Families of allatoregulator sequences: a 2011 perspective¹This review is part of a virtual symposium on recent advances in understanding a variety of complex regulatory processes in insect physiology and endocrinology, including development, metabolism, cold hardiness, food intake and digestion, and diuresis, through the use of omics technologies in the postgenomic era.

BendenaWilliam

TobeStephen

2012

Can. J. Zool.

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Three different peptide families have been named "allatostatins" (ASTs), based on their initial purifications which were based on their ability to inhibit juvenile hormone (JH) biosynthesis. These include (i) a family of peptides that have a consensus C-terminal sequence Y/FXFGL-NH 2 ; (ii) a family of peptides with a conserved C-terminal sequence W(X) 6 W-NH 2 ; and(iii) a family of peptides with C-terminal sequence PISCF, some of which are C-terminally-amidated. Each allatostatin family has functions distinct and apart from the inhibition of JH biosynthesis. A peptide family known as the "allatotropins" serve to stimulate JH biosynthesis. This family of peptides also has been proven to exert multiple effects dependent on the species in question. Genome and peptidome projects are uncovering new members of these families and it is clear that these structures are not just confined to Insecta but are found in a range of invertebrates. The receptors for these neuropeptides have been identified and tested experimentally for specific ligand binding. The Y/FXFGLa-ASTs exert their action through galanin-like receptors, W(X) 6 Wa-ASTs through a sex peptide-binding receptor, and PISCF-ASTs through somatostatin-like receptors. These receptors are conserved through evolutionary time and are being identified in numerous invertebrates by way of genome projects.Key words: allatostatin, allatotropin, juvenile hormone, muscle contraction, neuropeptides, neuropeptide receptors, insects, crustaceans, arthropods.Résumé : Trois familles différentes de peptides portent le nom d'« allatostatines » (AST) d'après leurs purifications initiales basées sur leur capacité d'inhiber la biosynthèse de l'hormone juvénile (JH). Elles incluent (i) une famille de peptides qui possèdent une séquence consensus C-terminale Y/FXFGL-NH 2 , (ii) une famille de peptides avec une séquence C-terminale conservée W(X) 6 W-NH 2 et (iii) une famille de peptides avec une séquence C-terminale PISCF, dont certains sont amidatées sur l'extrémité C-terminale. Chaque famille d'allatostatines possède des fonctions distinctes et additionnelles à l'inhibition de la synthèse de l'hormone juvénile. Une famille de peptides connus sous le nom d'« allatotropines » sert à stimuler la synthèse de la JH. Cette famille de peptides produit aussi des effets multiples selon l'espèce concernée. Des projets sur le génome et le peptidome découvrent de nouveaux membres de ces familles et il est clair que ces structures ne se retrouvent pas seulement chez les insectes, mais aussi chez une gamme d'invertébrés. Les récepteurs de ces neuropeptides ont été identifiés et ont été testé expérimentalement pour des liaisons spécifiques au ligand. Les AST-Y/FXFGLa exercent leur action par des récepteurs de type galanine, les AST-W(X)6Wa par un récepteur liant un peptide sexuel et les AST-PISCF par des récepteurs de type somatostatine. Ces récepteurs sont conservés au cours de l'évolution et sont présentement retrouvés chez de nombreux invertébrés dans le cadre des projets de génome.

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Molecular cloning of the gene for the allatostatin family of neuropeptides from the cockroach Diploptera punctata.

Cited by 148 publications

References 20 publications

Molecular cloning and characterization of cDNA for insect biogenic peptide, growth‐blocking peptide

Molecular cloning and characterization of cDNA for insect biogenic peptide, growth‐blocking peptide

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