Molecular cloning of the full‐length cDNA encoding the human calbindin‐D<sub>9k</sub>

Jeung, Eui‐Bae; Krisinger, John; Dann, Jamie L.; Leung, Peter C.K.

doi:10.1016/0014-5793(92)80772-9

Cited by 36 publications

(14 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CaBP9k mRNA was also reported by Glazier et al (2000) in cytotrophoblast cells and tissues, while Müller et al (2000) described the presence of a robust expression of CaBP9k mRNA in the whole human placenta. In contrast, Jeung et al (1992Jeung et al ( , 1994 and Howard et al (1992) argued for the complete absence of CaBP9k expression in human placenta. Therefore, we examined the presence and the expression levels of a CaBP9k transcript in both undifferentiated and differentiated trophoblast cells of the human term placenta.…”

Section: Introductionmentioning

confidence: 81%

Calbindin-D9k (CaBP9k) localization and levels of expression in trophoblast cells from human term placenta

Belkacemi

Gariépy

Mounier

et al. 2004

Cell and Tissue Research

View full text Add to dashboard Cite

During pregnancy, the calcium (Ca(2+)) transport machinery of the placenta is solely responsible for the nutrient supply to the developing fetus, where active Ca(2+) transport occurs from the mother to the fetus. As part of a larger study to determine the role of Ca(2+) in placental transport in vivo, we questioned whether calbindin-D9k (CaBP9k), which is mainly expressed in duodenum, uterus, and placenta of several mammals, is present in cytotrophoblast cells and syncytiotrophoblasts of human term placenta. We were interested in this protein because of its potential importance in serving as an indicator of Ca(2+) availability and utilization in the placenta. Here, we demonstrated that CaBP9k transcript is present in both cell types, with a lower expression in cytotrophoblast cells as compared to syncytiotrophoblasts. Moreover, we showed by immunochemistry that CaBP9k protein was present in cytotrophoblast and syncytiotrophoblast placental tissue sections as well as in cultured cells. The occurrence of CaBP9k protein in trophoblast cells was further confirmed by Western blot analysis. Thus, these results indicate for the first time that CaBP9k is unequivocally expressed by trophoblast cells from human term placenta.

show abstract

Section: Introductionmentioning

confidence: 81%

Calbindin-D9k (CaBP9k) localization and levels of expression in trophoblast cells from human term placenta

Belkacemi

Gariépy

Mounier

et al. 2004

Cell and Tissue Research

View full text Add to dashboard Cite

show abstract

“…The genes encoding not only rodent CaBP-9k but also human CaBP-9k have been cloned, and the complete sequence was established in our previous reports [33,34]. In the uterus of rats, it has been demonstrated that CaBP-9k gene expression is highly regulated by E2, and is known to fluctuate during the estrous cycle of rats when the serum E2 level is also fluctuating [35].…”

mentioning

confidence: 99%

Conflict of Estrogenic Activity by Various Phthalates between In Vitro and In Vivo Models Related to the Expression of Calbindin-D9k

Hong

Choi

et al. 2005

Journal of Reproduction and Development

Self Cite

View full text Add to dashboard Cite

Abstract.Phthalates are suspected to disrupt the endocrine system, especially through estrogenic effects. In the present study, we investigated the effects of various phthalates and compared them with those of estrogenic compounds that disrupt the female reproductive system. To assess the effects of these phthalates, alteration of the Calbindin-D9k (CaBP-9k) gene was measured as a biomarker because rat CaBP-9k gene carries an estrogen response element (ERE) which is involved in estrogen responsiveness of the gene during the estrous cycle. In this study, phthalates were tested for estrogenic properties in in vitro and in vivo models. First, the E-Screen assay was used to measure the proliferation of MCF-7 cells, a human breast cancer cell line. Treatments with 17β-estradiol (E2; 9-fold) and 17α-estradiol (EE; 9-fold) induced MCF-7 cell proliferation at concentrations of 10 -9 M. Phthalates induced an increase in MCF-7 proliferation at concentration of 10 -6 M up to 10 -4 M. Nbutyl benzyl phthalate (BBP; 6-fold vs. vehicle), dicyclohexyl phthalate (DCHP; 8-fold), 2-ethylhexyl phthalate (DEHP; 6-fold) and di-n-butyl phthalate (DBP; 7-fold) at the concentration of 10 -4 M induced in an increase in MCF-7 proliferation after 6 d of treatment compared to vehicle. However, significant increase in MCF-7 proliferation was induced by diethyl phthalate (DEP). Second, we investigated the expression of CaBP-9k in the uterus of immature rats after oral treatment with BBP, DCHP, DEHP, DBP or DBP (600 mg/kg per day) in this in vivo model, because the immature rat model is highly sensitive to exposure to estrogenic chemicals. None of the phthalates induced the expression of CaBP9k mRNA and its protein in the neonatal uterus as analysed by Northern and Western blot analyses, respectively. Although phthalates induced an increase in MCF-7 cell proliferation by an estrogenic effect, they could not induce CaBP-9k expression in the in vivo system, suggesting that the assays of estrogenic effects of various phthalates conducted in vitro and in vivo expression of CaBP-9k may produce conflicting results.

show abstract

“…This lack of expression was attributed to mutations in the 13 nucleotide long estrogen response element (ERE) at the 5 0 end of the baboon intron (Opperman et al 1990). In humans, the CaBP-d9k gene is 80% homologous to baboon CaBP-d9k and also contains the mutated ERE which would render the gene inactive in the uterus (Jeung et al 1992(Jeung et al , 1994. This is hypothesized to explain the lack of CaBP-d9k mRNA in human endometrium.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have identified that CaBP-d9k is highly expressed in the mouse and rat uterus during early pregnancy (Darwish et al 1991, Krisinger et al 1992, Krisinger et al 1993, L'Horset et al 1993, Tatsumi et al 1999, Nie et al 2000. In the mouse it is differentially expressed between implantation and inter-implantation sites at the time of initial embryo attachment (Nie et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Endometrial expression of calbindin (CaBP)-d28k but not CaBP-d9k in primates implies evolutionary changes and functional redundancy of calbindins at implantation

et al. 2004

View full text Add to dashboard Cite

The endometrium is hostile to embryo implantation except during the 'window of receptivity'. A change in endometrial gene expression is required for the development of receptivity. Calbindin-d9k (CaBP-d9k) and calbindin-d28k (CaBP-d28k) are proteins possessing EF-hand motifs which have high affinity for Ca 21 ions. Previously, it has been demonstrated that, in mouse endometrium, the expression of both calbindins is highly regulated during implantation and that both proteins play critical but functionally redundant roles at implantation. This study was the first to determine the expression of these two calbindins in the human and rhesus monkey endometrium. Initial RT-PCR analysis demonstrated that CaBP-d28k but not CaBP-d9k mRNA expression is detectable in the endometrium of both species. Western blot analysis confirmed the presence of immunoreactive CaBP-d28k protein in the primate endometrium. Furthermore, the endometrial expression pattern of CaBP-d28k mRNA and protein was examined by Northern blot analysis and immunohistochemistry respectively in both species across the menstrual cycle and during early pregnancy. Semi-quantitative statistical analysis of the immunohistochemistry results revealed that, in the human, CaBP-d28k protein expression was maximal in luminal and glandular epithelium during the mid-secretory phase, coinciding with the time when the endometrium is receptive to embryo implantation. Expression in rhesus monkey showed a similar trend. These results suggest that, in the primate endometrium, only CaBP-d28k is expressed and that the specific regulation of this calbindin is potentially important for the establishment of uterine receptivity.

show abstract

Molecular cloning of the full‐length cDNA encoding the human calbindin‐D_9k

Cited by 36 publications

References 25 publications

Calbindin-D9k (CaBP9k) localization and levels of expression in trophoblast cells from human term placenta

Calbindin-D9k (CaBP9k) localization and levels of expression in trophoblast cells from human term placenta

Conflict of Estrogenic Activity by Various Phthalates between In Vitro and In Vivo Models Related to the Expression of Calbindin-D9k

Endometrial expression of calbindin (CaBP)-d28k but not CaBP-d9k in primates implies evolutionary changes and functional redundancy of calbindins at implantation

Contact Info

Product

Resources

About

Molecular cloning of the full‐length cDNA encoding the human calbindin‐D9k

Cited by 36 publications

References 25 publications

Calbindin-D9k (CaBP9k) localization and levels of expression in trophoblast cells from human term placenta

Calbindin-D9k (CaBP9k) localization and levels of expression in trophoblast cells from human term placenta

Conflict of Estrogenic Activity by Various Phthalates between In Vitro and In Vivo Models Related to the Expression of Calbindin-D9k

Endometrial expression of calbindin (CaBP)-d28k but not CaBP-d9k in primates implies evolutionary changes and functional redundancy of calbindins at implantation

Contact Info

Product

Resources

About

Molecular cloning of the full‐length cDNA encoding the human calbindin‐D_9k