Molecular cloning of Ra-sHSPI, a novel member of the HSP20 family from Rhipicephalus annulatus salivary glands

Hussein, Nahla A.; Shahein, Yasser E.; El-Hakim, Amr E.; Abouelella, Amira M.; Guneidy, Rasha A.; Hamed, Ragaa R.

doi:10.1016/j.ijbiomac.2014.02.057

Cited by 6 publications

(3 citation statements)

References 51 publications

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“…Notably, HSPs and molecular chaperones were enriched in the transcriptome, supporting the involvement of HSPs in the physiological activities of ticks, especially blood-feeding [ 27 , 67 ]. However, using HSPs as a candidate vaccine antigen against ticks is still under scrutiny and needs extensive investigation [ 67 , 68 ]. In the tick Dermacentor silvarum , HSP70—and not HSP90—was required for adaptation to cold stress, leading to an understanding of the acclimatization of overwintering ticks [ 69 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of Haemaphysalis flava female using Illumina HiSeq 4000 sequencing: de novo assembly, functional annotation and discovery of SSR markers

Sang,

Patnaik,

Park

et al. 2023

Parasites Vectors

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Background Ticks are ectoparasites capable of directly damaging their hosts and transmitting vector-borne diseases. The ixodid tick Haemaphysalis flava has a broad distribution that extends from East to South Asia. This tick is a reservoir of severe fever with thrombocytopenia syndrome virus (SFTSV) that causes severe hemorrhagic disease, with cases reported from China, Japan and South Korea. Recently, the distribution of H. flava in South Korea was found to overlap with the occurrence of SFTSV. Methods This study was undertaken to discover the molecular resources of H. flava female ticks using the Illumina HiSeq 4000 system, the Trinity de novo sequence assembler and annotation against public databases. The locally curated Protostome database (PANM-DB) was used to screen the putative adaptation-related transcripts classified to gene families, such as angiotensin-converting enzyme, aquaporin, adenylate cyclase, AMP-activated protein kinase, glutamate receptors, heat shock proteins, molecular chaperones, insulin receptor, mitogen-activated protein kinase and solute carrier family proteins. Also, the repeats and simple sequence repeats (SSRs) were screened from the unigenes using RepeatMasker (v4.0.6) and MISA (v1.0) software tools, followed by the designing of SSRs flanking primers using BatchPrimer 3 (v1.0) software. Results The transcriptome produced a total of 69,822 unigenes, of which 46,175 annotated to the homologous proteins in the PANM-DB. The unigenes were also mapped to the EuKaryotic Orthologous Groups (KOG), Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) specializations. Promiscuous presence of protein kinase, zinc finger (C2H2-type), reverse transcriptase, and RNA recognition motif domains was observed in the unigenes. A total of 3480 SSRs were screened, of which 1907 and 1274 were found as tri- and dinucleotide repeats, respectively. A list of primer sequences flanking the SSR motifs was detailed for validation of polymorphism in H. flava and the related tick species. Conclusions The reference transcriptome information on H. flava female ticks will be useful for an enriched understanding of tick biology, its competency to act as a vector and the study of species diversity related to disease transmission. Graphical abstract

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Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of Haemaphysalis flava female using Illumina HiSeq 4000 sequencing: de novo assembly, functional annotation and discovery of SSR markers

Sang,

Patnaik,

Park

et al. 2023

Parasites Vectors

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Section: Degs Between Ticks Fed On Tick-susceptible and Resistant Cattlementioning

confidence: 90%

“…A transcript upregulated in ticks fed on tick-susceptible cattle showed similarity with the sequence of a small heat shock protein I gene cloned from salivary glands of Rhipicephalus annulatusthe RasHSPI (Hussein et al, 2014). The authors verified a strong immunogenic effect of the RasHSPI recombinant protein in rabbits, suggesting that it could be used as a potential protective antigen (Hussein et al, 2014). Previously, another sHSP also cloned from R. annulatus salivary glands, a RasHSPII, showed a role as a molecular chaperone, conferring protection at least or specifically to the tick salivary glands against the increase in temperature and other stresses observed during blood feeding by ticks (Shahein et al, 2010).…”

Section: Degs Between Ticks Fed On Tick-susceptible and Resistant Cattlementioning

confidence: 91%

Gene Expression in the Salivary Gland of Rhipicephalus (Boophilus) microplus Fed on Tick-Susceptible and Tick-Resistant Hosts

Giachetto

Cunha

Nhani

et al. 2020

Front. Cell. Infect. Microbiol.

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The success of cattle tick fixation largely depends on the secretion of substances that alter the immune response of the host. The majority of these substances are expressed by the parasite salivary gland and secreted in tick saliva. It is known that hosts can mount immune responses against ticks and bovine European breeds, and bovine industrial crossbreeds are more susceptible to infestations than are Bos indicus cattle. To identify candidates for the development of novel control strategies for the cattle tick Rhipicephalus (Boophilus) microplus, a salivary gland transcriptome analysis of engorged females fed on susceptible or resistant hosts was performed. Using RNA-Seq, transcriptomes were de novo assembled and produced a total of 235,451 contigs with 93.3% transcriptome completeness. Differential expression analysis identified 137 sequences as differentially expressed genes (DEGs) between ticks raised on tick-susceptible or tick-resistant cattle. DEGs predicted to be secreted proteins include innexins, which are transmembrane proteins that form gap junction channels; the transporters Na + /dicarboxylate, Na + /tricarboxylate, and phosphate transporter and a putative monocarboxylate transporter; a phosphoinositol 4-phosphate adaptor protein; a cysteine-rich protein containing a trypsin inhibitor-like (TIL) domain; a putative defense protein 3 containing a reeler domain; and an F-actin-uncapping protein LRRC16A with a CARMIL_C domain; these genes were upregulated in ticks fed on tick-susceptible cattle. DEGs predicted to be non-secreted proteins included a small heat shock protein and the negative elongation factor B-like, both acting in a coordinated manner to increase HSP transcript levels in the salivary glands of the ticks fed on tick-susceptible cattle; the 26S protease regulatory subunit 6B and another chaperone with similarity to calnexin, also upregulated in ticks fed on tick-susceptible cattle; an EF-hand calcium binding protein and a serine carboxypeptidase (SCP), both involved in the blood coagulation cascade and upregulated in ticks fed on tick-susceptible cattle; and two ribosomal proteins, the 60S acidic ribosomal protein P2 and the 60S ribosomal protein L19. These results help to characterize cattle tick salivary gland gene expression in tick-susceptible and tick-resistant hosts and suggest new putative targets for the control of tick infestations, as those genes involved in the mechanism of stress response during blood feeding.

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Heat Shock Proteins in Vector-pathogen Interactions: The Anaplasma phagocytophilum Model

Espinosa

Alberdi

Villar

et al. 2017

Heat Shock Proteins

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Molecular cloning of Ra-sHSPI, a novel member of the HSP20 family from Rhipicephalus annulatus salivary glands

Cited by 6 publications

References 51 publications

Transcriptome analysis of Haemaphysalis flava female using Illumina HiSeq 4000 sequencing: de novo assembly, functional annotation and discovery of SSR markers

Transcriptome analysis of Haemaphysalis flava female using Illumina HiSeq 4000 sequencing: de novo assembly, functional annotation and discovery of SSR markers

Gene Expression in the Salivary Gland of Rhipicephalus (Boophilus) microplus Fed on Tick-Susceptible and Tick-Resistant Hosts

Heat Shock Proteins in Vector-pathogen Interactions: The Anaplasma phagocytophilum Model

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