Molecular cloning of Proteus mirabilis uroepithelial cell adherence (uca) genes

Cook, Susan W.; Mody, Neeta S.; Valle, Jaione; Hull, Richard A.

doi:10.1128/iai.63.5.2082-2086.1995

Cited by 40 publications

(23 citation statements)

References 40 publications

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“…In fact, MR/P seems to act as a cofactor in the establishment of the AA pattern, because the lack of MR/P expression partially reduced that phenotype. It may be possible that any of the other adhesins already described in P. mirabilis Cook et al, 1995;Zunino et al, 2000) participates in this multifactorial phenotype in P. mirabilis. The AA pattern in EAEC is also a multifactorial phenotype (Moreira et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

“…Because this is a kidney-derived cell line, such an observation suggests the in vivo expression of this phenotype. Studies suggesting fimbriae-mediated P. mirabilis adhesion to tissue culture cells in vitro were reported by some authors (Sareneva et al, 1990;Cook et al, 1995;Jansen et al, 2004), but none of them described a characteristic pattern of adherence. In addition to EAEC, the AA pattern has been recognized in Klebsiella pneumoniae strains associated with nosocomial infections, and in atypical enteropathogenic and Shiga toxin-producing E. coli isolated from diarrhoea (Favre-Bonte et al, 1995;Morabito et al, 1998;Gomes et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Studies demonstrating fimbriae-mediated adhesion of P. mirabilis to tissue culture cells in vitro have been reported by some authors, but none of them described a characteristic pattern of adherence (Sareneva et al, 1990;Cook et al, 1995). In this study, the in vitro adherence pattern of P. mirabilis strains isolated from patients with UTI, and the establishment of the AA pattern displayed by these isolates were investigated.…”

Section: Introductionmentioning

confidence: 91%

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Aggregative adherence of uropathogenicProteus mirabilisto cultured epithelial cells

Rocha

Elias²,

Cianciarullo

et al. 2007

FEMS Immunology &Amp; Medical Microbiology

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Proteus mirabilis is an important cause of urinary tract infection (UTI) in patients with complicated urinary tracts. Thirty-five strains of P. mirabilis isolated from UTI were examined for the adherence capacity to epithelial cells. All isolates displayed the aggregative adherence (AA) to HEp-2 cells, a phenotype similarly presented in LLC-MK(2) cells. Biofilm formation on polystyrene was also observed in all strains. The mannose-resistant Proteus-like fimbriae (MR/P), Type I fimbriae and AAF/I, II and III fimbriae of enteroaggregative Escherichia coli were searched by the presence of their respective adhesin-encoding genes. Only the MR/P fimbrial subunits encoding genes mrpA and mrpH were detected in all isolates, as well as MR/P expression. A mutation in mrpA demonstrated that MR/P is involved in aggregative adherence to HEp-2 cells, as well as in biofilm formation. However, these phenotypes are multifactorial, because the mrpA mutation reduced but did not abolish both phenotypes. The present results reinforce the importance of MR/P as a virulence factor in P. mirabilis due to its association with AA and biofilm formation, which is an important step for the establishment of UTI in catheterized patients.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 91%

See 1 more Smart Citation

Aggregative adherence of uropathogenicProteus mirabilisto cultured epithelial cells

Rocha

Elias²,

Cianciarullo

et al. 2007

FEMS Immunology &Amp; Medical Microbiology

View full text Add to dashboard Cite

show abstract

“…These findings suggest that UCA was formerly an adhesin for the intestinal epithelium. The genetic organization of UCA biogenesis is still unknown and the only cloned gene thus far is ucaA, which encodes the subunit responsible for adhesion (Cook et al, 1995).…”

Section: Bacterial Adhesion To Uroepithelial Tractmentioning

confidence: 99%

Fimbriae of uropathogenicProteus mirabilis

Rocha

Pelayo

Elias

2007

FEMS Immunology &Amp; Medical Microbiology

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Prion diseases are zoonotic infectious diseases commonly transmissible among animals via prion infections with an accompanying deficiency of cellular prion protein (PrP(C)) and accumulation of an abnormal isoform of prion protein (PrP(Sc)), which are observed in neurons in the event of injury and disease. To understand the role of PrP(C) in the neuron in health and diseases, we have established an immortalized neuronal cell line HpL3-4 from primary hippocampal cells of prion protein (PrP) gene-deficient mice by using a retroviral vector encoding Simian Virus 40 Large T antigen (SV40 LTag). The HpL3-4 cells exhibit cell-type-specific proteins for the neuronal precursor lineage. Recently, this group and other groups have established PrP-deficient cell lines from many kinds of cell types including glia, fibroblasts and neuronal cells, which will have a broad range of applications in prion biology. In this review, we focus on recently obtained information about PrP functions and possible studies on prion infections using the PrPdeficient cell lines.

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“…These swarmer cells also exhibit reduced expression of fimbriae (Mobley et al, 1996;Jansen et al, 2003), suggesting possible co-ordinate expression between flagella and fimbriae. Prior to the sequencing of the P. mirabilis genome, expression of five different fimbriae had been documented in P. mirabilis: MR/P fimbriae (Adegbola et al, 1983;Bahrani et al, 1994), uroepithelial cell adhesin (UCA, also known as non-agglutinating fimbriae or NAF) (Wray et al, 1986;Cook et al, 1995), P. mirabilis fimbriae (PMF) (Adegbola et al, 1983;Massad et al, 1994a), ambient temperature fimbriae (ATF) (Massad et al, 1994b) and P. mirabilis P-like pili (PMP) (Bijlsma et al, 1995). The MR/P fimbria is encoded by an operon containing nine genes (Bahrani et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

Repression of motility during fimbrial expression: identification of 14 mrpJ gene paralogues in Proteus mirabilis

Pearson

Mobley

2008

Molecular Microbiology

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Summary Proteus mirabilis alternates between motile and adherent forms. MrpJ, a transcriptional regulator previously reported to repress motility, is encoded at the 3' end of the mrp fimbrial operon in P. mirabilis. Sequencing of the P. mirabilis genome revealed 14 additional paralogs of mrpJ, ten of which are associated with fimbrial operons. Twelve of these genes, when overexpressed, repressed motility; several distinct patterns of swarming motility were noted. Expression of 10 of the 14 mrpJ paralogs repressed flagellin (FlaA) synthesis. Alignment of the predicted amino acid sequences of MrpJ and its fourteen paralogs revealed a conserved consensus motif (SQQQFSRYE) within the helix-turn-helix domain. Site-directed mutagenesis of these residues coupled with linker insertion mutagenesis of MrpJ confirmed the importance of this domain for repression of motility. Gel shift assays demonstrated that MrpJ and another paralog UcaJ bind directly to the promoter region of the flagellar master regulator flhDC. Thus, P. mirabilis appears to use a related mechanism to inhibit motility during the production of at least ten of its predicted fimbriae.

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Molecular cloning of Proteus mirabilis uroepithelial cell adherence (uca) genes

Cited by 40 publications

References 40 publications

Aggregative adherence of uropathogenicProteus mirabilisto cultured epithelial cells

Aggregative adherence of uropathogenicProteus mirabilisto cultured epithelial cells

Fimbriae of uropathogenicProteus mirabilis

Repression of motility during fimbrial expression: identification of 14 mrpJ gene paralogues in Proteus mirabilis

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