Molecular Cloning of Pea cDNA Encoding Cycloartenol Synthase and Its Functional Expression in Yeast.

Morita, Masayo; Shibuya, Masabumi; Lee, Moon-Soon; Sankawa, Ushio; Ebizuka, Yutaka

doi:10.1248/bpb.20.770

Cited by 59 publications

(56 citation statements)

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“…An ORF of hOSC was inserted in the correct direction under the GPD promoter region to construct pYEG1h. The expression of hOSC and the preparation of the cell-free extracts were performed by the reported method [12][13][14] with some modification. The mutant yeast strain GIL77 (gal2 hem3-6 erg7 ura3-167) 12) was transformed with the resultant plasmid pYEG1h by Frozen-EZ Yeast Transformation II TM (Zymo Research).…”

Section: Methodsmentioning

confidence: 99%

Inhibition of Human Lanosterol Synthase by the Constituents of Colocasia esculenta (Taro)

Sakano

Mutsuga

Tanaka

et al. 2005

Biological & Pharmaceutical Bulletin

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Ethanol extracts of lyophilized vegetables were tested for inhibition of human lanosterol synthase (hOSC) in order to find the compounds to suppress cholesterol biosynthesis. Of 130 samples tested, twelve samples showed significant inhibition. Among them, Colocasia esculenta (taro) showed the highest inhibition (55% inhibition at 300 m mg/ml). Examination of activity variation among eight taro cultivars indicated that "Aichi-wase" and "Yatsugashira" had the most potent activity for hOSC inhibition. In order to identify the active constituent of taro, ethanol extracts of "Aichi-wase" were partitioned with hexane and aqueous methanol, and fractionated by silica gel column chromatography. Inhibitory activity was concentrated in two major active fractions. Further purification of these fractions by preparative HPLC gave three monogalactosyldiacylglycerols and five digalactosyldiacylglycerols as active compounds that showed 28 to 67% inhibitory activities at the concentration 300 m mg/ml.

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Section: Methodsmentioning

confidence: 99%

Inhibition of Human Lanosterol Synthase by the Constituents of Colocasia esculenta (Taro)

Sakano

Mutsuga

Tanaka

et al. 2005

Biological & Pharmaceutical Bulletin

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“…Following the conditions reported in previous papers [18,19], nested PCR was carried out with the single-stranded cDNA mixture as a template and the degenerate primers 162S (5…”

Section: Cdna Cloning Of Oscmentioning

confidence: 99%

“…LC-APCIMS was measured with LCQ (Thermo Fisher Scientific, MA, USA) equipped with a HP1100 series LC system (Agilent Technologies, CA, USA) under the same conditions as reported previously [19]. The spot corresponding to triterpene monoalcohol on TLC was subjected to LC-MS. HPLC was carried out using a SUPER-ODS column (/ 4.6 · 200 mm) (Tosoh, Tokyo, Japan) with 95% CH 3 CN aq.…”

Section: Lc-apcims Analysis Of the Acx Productmentioning

confidence: 99%

Squalene cyclase and oxidosqualene cyclase from a fern

et al. 2007

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Ferns are the most primitive vascular plants. The phytosterols of ferns are the same as those of higher plants, but they produce characteristic triterpenes. The most distinct feature is the lack of oxygen functionality at C-3, suggesting that the triterpenes of ferns may be biosynthesized by direct cyclization of squalene. To obtain some insights into the molecular bases for the biosynthesis of triterpenes in ferns, we cloned ACX, an oxidosqualene cyclase homologue, encoding a cycloartenol synthase (CAS) and ACH, a squalene cyclase homologue, encoding a 22-hydroxyhopane synthase from Adiantum capillus-veneris. Phylogenetic analysis revealed that ACH is located in the cluster of bacterial SCs, while ACX is in the cluster of higher plant CASs.

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“…In addition, cDNAs of cycloartenol synthase (EC 5.4.99.8), an OSC involved in phytosterol biosynthesis, have been cloned from different plant species. 9,[13][14][15][16][17][18][19] Although mechanistic studies on plant triterpene synthases have been reported, 20,21) the regulatory role of these OSCs in the triterpenoid biosynthesis has not been elucidated at the molecular level.…”

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confidence: 99%

Cloning and Characterization of a cDNA Encoding .BETA.-Amyrin Synthase Involved in Glycyrrhizin and Soyasaponin Biosyntheses in Licorice.

Hayashi

Huang

Kirakosyan

et al. 2001

Biological & Pharmaceutical Bulletin

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139

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Molecular Cloning of Pea cDNA Encoding Cycloartenol Synthase and Its Functional Expression in Yeast.

Cited by 59 publications

References 0 publications

Inhibition of Human Lanosterol Synthase by the Constituents of Colocasia esculenta (Taro)

Inhibition of Human Lanosterol Synthase by the Constituents of Colocasia esculenta (Taro)

Squalene cyclase and oxidosqualene cyclase from a fern

Cloning and Characterization of a cDNA Encoding .BETA.-Amyrin Synthase Involved in Glycyrrhizin and Soyasaponin Biosyntheses in Licorice.

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