Molecular cloning of partial 14-3-3 genes in the marine sponge Hymeniacidon perleve and its role in differentiating infectious and non-infectious bacteria

Fu, Wantao; Zhang, JuLin; Chen, Zheng; Liu, Jing; An, ZhongFu; Liu, HongWen; Zhang, Wei

doi:10.1007/s11434-012-5400-z

Cited by 3 publications

(2 citation statements)

References 49 publications

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“…However, other than the use of antimicrobial compounds, sponges possess efficient defense mechanisms that recognize pathogens and initiate an immune response. Sponges distinguish between infectious and non-infectious bacteria through molecular responses, receptor molecules and membrane proteins [ 115 – 117 ]. Bacteria associated with sponges are able to produce molecules which act on the sponge cells by inhibiting its immune and apoptotic system [ 118 – 121 ].…”

Section: Discussionmentioning

confidence: 99%

Sponge chemical defenses are a possible mechanism for increasing sponge abundance on reefs in Zanzibar

et al. 2018

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Coral reefs are experiencing increasing anthropogenic impacts that result in substantial declines of reef-building corals and a change of community structure towards other benthic invertebrates or macroalgae. Reefs around Zanzibar are exposed to untreated sewage and runoff from the main city Stonetown. At many of these sites, sponge cover has increased over the last years. Sponges are one of the top spatial competitors on reefs worldwide. Their success is, in part, dependent on their strong chemical defenses against predators, microbial attacks and other sessile benthic competitors. This is the first study that investigates the bioactive properties of sponge species in the Western Indian Ocean region. Crude extracts of the ten most dominant sponge species were assessed for their chemical defenses against 35 bacterial strains (nine known as marine pathogens) using disc diffusion assays and general cytotoxic activities were assessed with brine shrimp lethality assays. The three chemically most active sponge species were additionally tested for their allelopathic properties against the scleractinian coral competitor Porites sp.. The antimicrobial assays revealed that all tested sponge extracts had strong antimicrobial properties and that the majority (80%) of the tested sponges were equally defended against pathogenic and environmental bacterial strains. Additionally, seven out of ten sponge species exhibited cytotoxic activities in the brine shrimp assay. Moreover, we could also show that the three most bioactive sponge species were able to decrease the photosynthetic performance of the coral symbionts and thus were likely to impair the coral physiology.

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Section: Discussionmentioning

confidence: 99%

Sponge chemical defenses are a possible mechanism for increasing sponge abundance on reefs in Zanzibar

et al. 2018

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“…Sponges can succumb to bacterial infection where their cells become overrun with bacteria causing death to the organism [36]. Fu et al [37] demonstrated the infection of the marine sponge Hymeniacidon perleve with Vibrio spp. through genetic markers for cell death.…”

Section: Discussionmentioning

confidence: 99%

How suitable is freshwater sponge Ephydatia fluviatilis (Linnaeus, 1759) for time-integrated biomonitoring of microbial water quality?

Cartwright,

Dooley,

McGonigle

et al. 2024

Preprint

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Faecal pollution of water by bacteria has a negative effect on water quality and can pose a potential health hazard. Conventional surveillance of microbial water quality relies on the analysis of low frequency spot samples and is thus likely to miss episodic or periodic pollution. This study aimed to investigate the potential of filter feeding sponges for time-integrated biomonitoring of microbial water quality. Laboratory trials tested the effects of different ratios of bacterial abundance and the sequence of exposure on bacterial retention by the freshwater sponge Ephydatia fluviatilis (Linnaeus, 1759) to establish its potential to indicate bacterial exposure. Gemmule grown sponges were simultaneously exposed to Escherichia coli and Enterococcus faecalis but at different ratios (Trial 1) or individually exposed to each bacterial species but in different sequential order (Trial 2). The E. coli and E. faecalis retained in each sponge was quantified by culture on selective agars. Data analysis was conducted using the Kruskal- Wallis test and/ or the Mann Whitney U test to compare between the numbers of bacteria retained in each treatment. Additionally, the Wilcoxon Matched-Paired Signed-Rank test was used for comparison of the different bacterial abundances retained within each individual sponge. Sponges from all trials retained E. coli and E. faecalis in small numbers relative to the exposure (<0.05% Trial 1 and <0.07% Trial 2) but exhibited higher retention of E. coli. Higher abundance of either bacterial species resulted in significantly lower (p<0.005) retention of the same species within sponges (Trial 1). An initial exposure to E. coli resulted in significantly higher (p=0.040) retention of both bacterial species than when sponges were exposed to E. faecalis first (Trial 2). Bacterial retention by sponges was neither representative of bacterial abundance in the ambient water nor the sequence of exposure. This implies either selective filtration or an attempt by sponges to prevent infection. However, freshwater sponges may still be useful in biomonitoring as qualitative time-integrated samplers of faecal indicator bacteria.

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How suitable is freshwater sponge Ephydatia fluviatilis (Linnaeus, 1759) for time-integrated biomonitoring of microbial water quality?

Cartwright,

Dooley,

McGonigle

et al. 2024

Access Microbiology

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Faecal pollution of water by bacteria has a negative effect on water quality and can pose a potential health hazard. Conventional surveillance of microbial water quality relies on the analysis of low-frequency spot samples and is thus likely to miss episodic or periodic pollution. This study aimed to investigate the potential of filter-feeding sponges for time-integrated biomonitoring of microbial water quality. Laboratory trials tested the effects of different ratios of bacterial abundance and the sequence of exposure on bacterial retention by the freshwater sponge Ephydatia fluviatilis (Linnaeus, 1759) to establish its potential to indicate bacterial exposure. Gemmule grown sponges were simultaneously exposed to Escherichia coli and Enterococcus faecalis but at different ratios (Trial 1) or individually exposed to each bacterial species but in different sequential order (Trial 2). The E. coli and E. faecalis retained in each sponge was quantified by culture on selective agars. Data analysis was conducted using the Kruskal–Wallis test and/or the Mann–Whitney U test to compare between the numbers of bacteria retained in each treatment. Additionally, the Wilcoxon matched-paired signed-rank test was used for comparison of the different bacterial abundances retained within each individual sponge. Sponges from all trials retained E. coli and E. faecalis in small numbers relative to the exposure (<0.05 % Trial 1 and <0.07 % Trial 2) but exhibited higher retention of E. coli. Higher abundance of either bacterial species resulted in significantly lower (P<0.005) retention of the same species within sponges (Trial 1). An initial exposure to E. coli resulted in significantly higher (P=0.040) retention of both bacterial species than when sponges were exposed to E. faecalis first (Trial 2).Bacterial retention by sponges was neither quantitatively representative of bacterial abundance in the ambient water nor the sequence of exposure. This implies either selective filtration or an attempt by sponges to prevent infection. However, freshwater sponges may still be useful in biomonitoring as qualitative time-integrated samplers of faecal indicator bacteria as they detect different bacteria present in the water even if their quantities cannot be estimated.

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Molecular cloning of partial 14-3-3 genes in the marine sponge Hymeniacidon perleve and its role in differentiating infectious and non-infectious bacteria

Cited by 3 publications

References 49 publications

Sponge chemical defenses are a possible mechanism for increasing sponge abundance on reefs in Zanzibar

Sponge chemical defenses are a possible mechanism for increasing sponge abundance on reefs in Zanzibar

How suitable is freshwater sponge Ephydatia fluviatilis (Linnaeus, 1759) for time-integrated biomonitoring of microbial water quality?

How suitable is freshwater sponge Ephydatia fluviatilis (Linnaeus, 1759) for time-integrated biomonitoring of microbial water quality?

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