Molecular cloning of P450 aromatase from the leopard gecko and its expression in the ovary

Endo, Daisuke; Park, Min Kyun

doi:10.1016/j.jsbmb.2005.02.015

Cited by 10 publications

(5 citation statements)

References 40 publications

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“…Furthermore, we found that p450arom was also transcribed in testes in 30-mm cane toads, similar to the expression pattern seen in some other vertebrates such as the leopard gecko (Endo and Park, 2005), the frog X. laevis (Miyashita et al, 2000), and the mouse, where it is required for spermatogenesis (Robertson et al, 1999). p450arom expression is also influenced by seasonal and hormonal fluctuations (Nunez and Applebaum, 2006), likely explaining the high variation we observe in our wild-caught samples.…”

Section: Discussionsupporting

confidence: 86%

“…The conserved gonad-specific expression pattern in many vertebrate lineages reflects its ancient association with sex-determination and sexual differentiation within both invertebrates and vertebrates (Raymond et al, 1998. p450arom showed a wider range of tissue expression, with transcripts detected in several tissues including the gonads and the brain, the two characteristic localizations of expression in all species studied to date (Kuntz et al, 2003;Endo and Park, 2005;van Nes and Andersen, 2006). Many species of teleost fish express a second isoform of p450arom in the brain.…”

Section: Discussionmentioning

confidence: 98%

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Cloning and expression of candidate sexual development genes in the cane toad (Bufo marinus)

Abramyan

Feng

Koopman

2009

Developmental Dynamics

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The development of the reproductive system in bufonids (true toads) is unique in several respects: sexual differentiation occurs later than in other anurans, and toads develop a Bidder's organ, a rudimentary ovary that can be manipulated in males to produce mature oocytes. To illuminate the genesis of this unusual reproductive system, we isolated from the cane toad (Bufo marinus) the orthologues of several known vertebrate sex-determining genes, determined their primary structure, and studied their expression by reverse transcriptase-polymerase chain reaction and in situ hybridization of tissue sections. We report here that cane toad Sox9, Dmrt1, and p450aromatase (Cyp19a1) are highly homologous to their counterparts in other vertebrates. They show profiles of expression that generally follow patterns observed in other taxa, but with some novel features. Our data suggest that these genes likely play key roles in sex determination and early gonad development in bufonids.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 98%

Cloning and expression of candidate sexual development genes in the cane toad (Bufo marinus)

Abramyan

Feng

Koopman

2009

Developmental Dynamics

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“…The 17β-estradiol synthesis may not involve the functional cooperation between the theca and granulosa cells, as this was noted in some species such as chicken where P450 aromatase was expressed only in the theca [25]. In the pig [26], fish [27] and lizard [28], the P450 aromatase was found both in the theca and granulosa.…”

Section: Discussionmentioning

confidence: 92%

Correlation between ovarian steroidogenesis and beta-endorphin in the Lizard Uromastyx acanthinura: Immunohistochemical approach.

Hammouche

Gernigon²,

Exbrayat³

2010

Folia Histochem Cytobiol.

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Abstract:In Mammals, opioid peptides are involved in various physiological processes including the reproductive function. The knowledge of the distribution of β-endorphin, one of opioid peptides in Reptiles ovaries is very limited. Therefore, the present study used the lizard ovarian follicles to further elucidate the role of this peptide in steroidogenesis. In Uromastyx acanthinura, the localization of both this peptide and sex steroid hormone was investigated by the immunohistochemical approach. This technique was used to evaluate the distribution of these substances and their relationship. The β-endorphin is strongly distributed in the granulosa cells and oocyte cytoplasm of the previtellogenic follicles in sexually quiescent lizards (winter) when steroidogenesis was interrupted. In spring, the signal became weak, or even absent, in the vitellogenic and previtellogenic follicles. The granulosa cells of the previtellogenic follicles showed an important synthesis of 17β-estradiol. Females that did not undergo in vitellogenesis in spring showed the same profile than quiescent females of winter. These findings represent the first evidence of the presence of β-endorphin in the ovary of this lizard. The seasonal variations observed in the reproductive cycle suggest that this opioid peptide is involved in the modulation of seasonal steroidogenesis.

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“…Moreover, Cyp19 encoding a P450 aromatase enzyme is highly conserved in all phyla of vertebrates (Tong and Chung, 2003). In mammals, Cyp19 is expressed in various tissues such as the ovary, testis, brain, placenta, and skin (Mahendroo et al, 1991); this is also true in reptiles (Endo and Park, 2005).…”

Section: Introductionmentioning

confidence: 99%

Sox3: A transcription factor for Cyp19 expression in the frog Rana rugosa

Oshima

Naruse

Nakamura

et al. 2009

Gene

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Molecular cloning of P450 aromatase from the leopard gecko and its expression in the ovary

Cited by 10 publications

References 40 publications

Cloning and expression of candidate sexual development genes in the cane toad (Bufo marinus)

Cloning and expression of candidate sexual development genes in the cane toad (Bufo marinus)

Correlation between ovarian steroidogenesis and beta-endorphin in the Lizard Uromastyx acanthinura: Immunohistochemical approach.

Sox3: A transcription factor for Cyp19 expression in the frog Rana rugosa

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