Molecular cloning of novel alternatively spliced variants of BCL2L12, a new member of the BCL2 gene family, and their expression analysis in cancer cells

Kontos, Christos K.; Scorilas, Andreas

doi:10.1016/j.gene.2012.04.084

Cited by 32 publications

(19 citation statements)

References 89 publications

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“…BCL2L12 is one of the most recently identified genes of this family, whose role in apoptosis has not been fully defined yet [35]. Although BCL2L12 expression has been found to be significantly affected by several antineoplastic drugs [36], in our experiments no remarkable alterations were noticed in its mRNA levels.…”

Section: Discussioncontrasting

confidence: 53%

Cytotoxic activity of sunitinib and everolimus in Caki-1 renal cancer cells is accompanied by modulations in the expression of apoptosis-related microRNA clusters and BCL2 family genes

Papadopoulos

Yousef

Scorilas

2015

Biomedicine & Pharmacotherapy

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Section: Discussioncontrasting

confidence: 53%

Cytotoxic activity of sunitinib and everolimus in Caki-1 renal cancer cells is accompanied by modulations in the expression of apoptosis-related microRNA clusters and BCL2 family genes

Papadopoulos

Yousef

Scorilas

2015

Biomedicine & Pharmacotherapy

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“…Each PARK2 splice variants may acts in different manner to suit cell specific needs. This hypothesis is supported by previous evidences showing different and even opposite functions of other splice variants, such as BCl2L12 pattern expression related to cellular phenotype [35]. Finally, based on the extensive alternative splicing process of PARK2 gene, we cannot rule out that additional splice variants with different functions (beyond those listed) may exist.…”

Section: Park2 Alternative Splice Transcripts Produce Isoforms Witmentioning

confidence: 54%

Alternative Splicing Generates Different Parkin Protein Isoforms: Evidences in Human, Rat, and Mouse Brain

Scuderi

Cognata

Drago

et al. 2014

BioMed Research International

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Parkinson protein 2, E3 ubiquitin protein ligase (PARK2) gene mutations are the most frequent causes of autosomal recessive early onset Parkinson's disease and juvenile Parkinson disease. Parkin deficiency has also been linked to other human pathologies, for example, sporadic Parkinson disease, Alzheimer disease, autism, and cancer. PARK2 primary transcript undergoes an extensive alternative splicing, which enhances transcriptomic diversification. To date several PARK2 splice variants have been identified; however, the expression and distribution of parkin isoforms have not been deeply investigated yet. Here, the currently known PARK2 gene transcripts and relative predicted encoded proteins in human, rat, and mouse are reviewed. By analyzing the literature, we highlight the existing data showing the presence of multiple parkin isoforms in the brain. Their expression emerges from conflicting results regarding the electrophoretic mobility of the protein, but it is also assumed from discrepant observations on the cellular and tissue distribution of parkin. Although the characterization of each predicted isoforms is complex, since they often diverge only for few amino acids, analysis of their expression patterns in the brain might account for the different pathogenetic effects linked to PARK2 gene mutations.

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“…Recent RNA-seq and proteomics studies have illuminated the magnitude of alternative splicing in mammals and the attendant proteomic and phenotypic diversity generated by this mechanism (Barbosa-Morais et al 2012). Different splice isoforms can have radically different functions, as for example pro-or anti-apoptotic splice variants of BCL2 (Kontos and Scorilas 2012). Limited systematic studies on dozens to hundreds of isoforms suggest that exon composition can often dramatically alter protein interaction profiles (Ellis et al 2012;Yang et al 2016).…”

Section: Evolution Of New Functionsmentioning

confidence: 99%

Contextual diversity of the human cell-essential proteome

Bertomeu

Coulombe-Huntington

Chatr‐aryamontri

et al. 2017

Preprint

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Essential genes define central biological functions required for cell growth, proliferation and survival, but the nature of gene essentiality across human cell types is not well understood. We assessed essential gene function in a Cas9-inducible human B-cell lymphoma cell line using an extended knockout (EKO) library of 278,754 sgRNAs that targeted 19,084 RefSeq genes, 20,852 alternatively-spliced exons and 3,872 hypothetical genes. A new statistical analysis tool called RANKS identified 2,280 essential genes, 234 of which had not been reported previously. Essential genes exhibited a bimodal distribution across 10 cell lines screened in different studies, consistent with a continuous variation in essentiality as a function of cell type. Genes essential in more lines were associated with more severe fitness defects and encoded the evolutionarily conserved structural cores of protein complexes. Genes essential in fewer lines tended to form context-specific modules and encode subunits at the periphery of essential complexes. The essentiality of individual protein residues across the proteome correlated with evolutionary conservation, structural burial, modular domains, and protein interaction interfaces. Many alternatively-spliced exons in essential genes were dispensable and tended to encode disordered regions. We also detected a significant fitness defect for 44 newly evolved hypothetical reading frames. These results illuminate the nature and evolution of essential gene functions in human cells.

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Molecular cloning of novel alternatively spliced variants of BCL2L12, a new member of the BCL2 gene family, and their expression analysis in cancer cells

Cited by 32 publications

References 89 publications

Cytotoxic activity of sunitinib and everolimus in Caki-1 renal cancer cells is accompanied by modulations in the expression of apoptosis-related microRNA clusters and BCL2 family genes

Cytotoxic activity of sunitinib and everolimus in Caki-1 renal cancer cells is accompanied by modulations in the expression of apoptosis-related microRNA clusters and BCL2 family genes

Alternative Splicing Generates Different Parkin Protein Isoforms: Evidences in Human, Rat, and Mouse Brain

Contextual diversity of the human cell-essential proteome

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