Molecular Cloning of Mouse Glycolate Oxidase

Kohler, Stefan A.; Menotti, Eric; Kühn, Lukas C.

doi:10.1074/jbc.274.4.2401

Cited by 52 publications

(6 citation statements)

References 51 publications

(39 reference statements)

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“…We then examined the expression of enzymes involved in the biosynthesis of oxalate in the liver and found that mRNA expression of GO (33, 34), but not that of alanine-glyoxylate aminotransferase 1 (AGT1) or alanine-glyoxylate aminotransferase 2 (AGT2), was higher in the liver tissues of the WT mice than in the TARKO mice and the Alb-ARKO mice (Figure 3D; P < .05 for TARKO/WT, P < .05 for Alb-ARKO/WT). Higher GO mRNA expression was then detected in human patients with kidney stones than in non–stone-forming male patients (Supplemental Figure 4; P = .001).…”

Section: Resultsmentioning

confidence: 99%

Androgen Receptor Enhances Kidney Stone-CaOx Crystal Formation via Modulation of Oxalate Biosynthesis & Oxidative Stress

Liang

Tian

et al. 2014

Molecular Endocrinology

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Males develop kidney stones far more frequently than females with a ratio of 2–3:1, suggesting that androgen receptor (AR) signaling might play a key role in the development of nephrolithiasis. Using the cre-loxP system to selectively knock out AR in glyoxylate-induced calcium oxalate (CaOx) crystal mouse models, we found that the mice lacking hepatic AR had less oxalate biosynthesis, which might lead to lower CaOx crystal formation, and that the mice lacking kidney proximal or distal epithelial AR also had lower CaOx crystal formation. We found that AR could directly up-regulate hepatic glycolate oxidase and kidney epithelial NADPH oxidase subunit p22-PHOX at the transcriptional level. This up-regulation might then increase oxalate biosynthesis and oxidative stress that resulted in induction of kidney tubular injury. Targeting AR with the AR degradation enhancer ASC-J9 led to suppression of CaOx crystal formation via modulation of oxalate biosynthesis and oxidative stress in both in vitro and in vivo studies. Taken together, these results established the roles of AR in CaOx crystal formation.

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Section: Resultsmentioning

confidence: 99%

Androgen Receptor Enhances Kidney Stone-CaOx Crystal Formation via Modulation of Oxalate Biosynthesis & Oxidative Stress

Liang

Tian

et al. 2014

Molecular Endocrinology

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“…The enzyme GO, also known as (L-2-) hydroxy-acid oxidase, which metabolizes GA to glyoxylate, is widely conserved in intestinal bacteria and mammals [33][34][35][36][37][38]. VB-diet feeding induced changes in the abundances of specific phylum, such as Firmicutes and Bacteroidetes; however, unlike the change in GA concentration, no consistent changes were observed between the 2-week and 4-week treatments (Supplemental Figure S5).…”

Section: Discussionmentioning

confidence: 99%

Glycolate is a Novel Marker of Vitamin B2 Deficiency Involved in Gut Microbe Metabolism in Mice

et al. 2020

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Microbes in the human gut play a role in the production of bioactive compounds, including some vitamins. Although several studies attempted to identify definitive markers for certain vitamin deficiencies, the role of gut microbiota in these deficiencies is unclear. To investigate the role of gut microbiota in deficiencies of four vitamins, B2, B6, folate, and B12, we conducted a comprehensive analysis of metabolites in mice treated and untreated with antibiotics. We identified glycolate (GA) as a novel marker of vitamin B2 (VB2) deficiency, and show that gut microbiota sense dietary VB2 deficiency and accumulate GA in response. The plasma GA concentration responded to reduced VB2 supply from both the gut microbiota and the diet. These results suggest that GA is a novel marker that can be used to assess whether or not the net supply of VB2 from dietary sources and gut microbiota is sufficient. We also found that gut microbiota can provide short-term compensation for host VB2 deficiency when dietary VB2 is withheld.

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“…IRE/IRP complexes also control translation of mRNAs encoding proteins of oxidative metabolism [(FeS)-cluster protein, mitochondrial aconitase, heme synthesis protein, erythroid 5′-aminolevulinic acid synthase (eALAS), succinate dehydrogenase ( Drosophila melanogaster ), hypoxia-inducible factor 2 (HIF2)], and phosphate signaling (kinase MRCKα and protein phosphatase, CDC14A and refs 81–83). Two of several recent reviews describing the IRE family are refs 14 and 69.…”

Section: Iron/dioxygen Regulation Of Translationmentioning

confidence: 99%

Living with Iron (and Oxygen): Questions and Answers about Iron Homeostasis

2009

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After early education in a one-room schoolhouse in rural Nebraska, Dixie J. Goss obtained her B.S. degree from Nebraska Wesleyan University and her Ph.D. degree from the University of Nebraska. Following a year of postdoctoral work at the University of Georgia, she returned to the University of Nebraska. She joined the Chemistry faculty of Hunter College, City University of New York, in 1984, was promoted to full professor in l991, and was appointed the Gertrude Elion Endowed Scholar in 2002. Her research interests include macromolecular assembly, regulation of translation, and kinetics of protein-RNA interactions.

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Molecular Cloning of Mouse Glycolate Oxidase

Cited by 52 publications

References 51 publications

Androgen Receptor Enhances Kidney Stone-CaOx Crystal Formation via Modulation of Oxalate Biosynthesis & Oxidative Stress

Androgen Receptor Enhances Kidney Stone-CaOx Crystal Formation via Modulation of Oxalate Biosynthesis & Oxidative Stress

Glycolate is a Novel Marker of Vitamin B2 Deficiency Involved in Gut Microbe Metabolism in Mice

Living with Iron (and Oxygen): Questions and Answers about Iron Homeostasis

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