Molecular cloning of Lyt-2, a membrane glycoprotein marking a subset of mouse T lymphocytes: molecular homology to its human counterpart, Leu-2/T8, and to immunoglobulin variable regions.

Abstract

The sequence of Lyt-2 cDNA shows that it is a new member of the immunoglobulin super gene family. Analysis of the predicted amino acid sequence indicates that the Lyt-2 polypeptide is synthesized with a 27-amino acid leader, and that the mature protein has an immunoglobulin variable region (Ig V)-related sequence of 100 amino acids, an extracellular spacer of 43, a transmembrane region of 38, and an intracytoplasmic region of 27 amino acids. Lyt-2 and its human analogue Leu-2 are 56% homologous; analysis indicates that the Ig V-related domains of the two molecules have evolved away from each other faster than the carboxyl-terminal half of the proteins.

Molecular characterization of the murine cytotoxic T-cell membrane glycoprotein Ly-3 (CD8).

“…In man CD8 appears to consist of homodimers of a single polypeptide chain of Mr 34,000 (6), whereas in the rat there are two chains: one (Mr 32,000) is equivalent to Ly-2 (7,8), and the second chain (Mr 37,000) seems to be equivalent to Ly-3 (7,9) but is clearly larger than the murine Ly-3(f3) chain. Recently, the murine Ly-2 gene has been cloned (3,10), and an analysis of genomic and cDNA clones and mRNA expression indicated the presence of a single gene, which by alternative splicing of the pre-mRNA, encodes different mRNAs for the a and a' polypeptides (3,11). To further pursue these studies and the relationship between the Ly-2 and Ly-3 antigens, we now report Ly-3.1 peptide sequences together with the isolation and molecular characterization of the cDNA coding for the murine Ly-3 molecule.…”

Involvement of cell cycle progression in survival signaling through CD40 in the B-lymphocyte line WEHI-231

“…Kitamura and Miyajima, University of Tokyo, Tokyo, Japan) 45 by a NotI-SalI fragment encoding mouse CD8 46 and a NotI-SalI fragment encoding YFP, respectively. The EcoRI-XhoI fragment containing kip1 cDNA was isolated from pBS-Kip1 20 and inserted into the EcoRIXhoI site of pMx-IRES-GFP and pMx-IRES-CD8, resulting in pMx-Kip1-IRES-GFP and pMx-Kip1-IRES-CD8, respectively (Figure 1).…”

Development of a modified selective amplifier gene for hematopoietic stem cell gene therapy

“…5 In ⌬Y703F-GCRER, phenylalanine was substituted for a cytoplasmic tyrosine (corresponding to position 703 in the murine G-CSFR), based on the results of mutation analysis by Yoshikawa et al 12 In the present study, ⌬GCRER and ⌬Y703F-GCRER cDNAs were cloned into bicistronic retroviral vectors harboring the murine CD8a gene downstream of the encephalomyocarditis virus (EMCV)-derived internal ribosome entry site (IRES). [15][16][17] As a result, each construct (pMX/⌬GCRER-IRES-CD8a or pMX/⌬Y703F-GCRER-IRES-CD8a) had one selective amplifier gene (⌬GCRER or ⌬Y703F-GCRER) to be expressed in a cap-dependent manner and CD8a to be IRES-dependent.…”

“…[15][16][17]30 pMX/MCAT-IRES-neo has the murine ecotropic receptor gene (MCAT-1; a gift from Dr JM Cunningham, Harvard Medical School, Boston, MA, USA) placed upstream of the IRES-neo cassette. 19 pMX/⌬GCRER-IRES-CD8a is a modified vector of pMX-⌬(5-195)GCRER described previously, for coexpression of deleted GCRER and CD8a.…”