Molecular cloning of leukocyte cell-derived chemotaxin 2 in rainbow trout

An interaction between leukocyte cell-derived chemotaxin 2 and transferrin of ayu, Plecoglossus altivelis

“…Human LECT2 is a 16-kDa basic protein with three intramolecular disulphide bonds, expressed preferentially in the liver in a constitutive manner and secreted into the bloodstream [2]. Proteins homologous to LECT2 have been isolated in many vertebrates in addition to humans, such as bovine (Bos taurus) [2], mouse (Mus musculus) [3], carp (Cyprinus carpio) [4], rainbow trout (Oncorhynchus mykiss) [5], chicken (Gallus gallus) [6], zebrafish (Danio rerio) [7] and croceine croaker (Larimichthys crocea) [8]. Current evidence suggests that LECT2 may be a multifunctional protein, involved in cell growth, differentiation, damage/ repair process and autoimmune response [9][10][11][12].…”

Molecular cloning of leucocyte cell-derived chemotaxin-2 gene in croceine croaker (Pseudosciaena crocea)

“…The 3 0 -UTR of pLECT2 cDNA was 112 bp long and contained a single typical polyadenylation signal ''AATAAA'' at nts 572e577. By contrast, the 3 0 -UTR of the LECT2 cDNA of trout was 210 bp long and contained two typical polyadenylation signal at nts 534e539 and 656e661 [8].…”

“…Current evidence suggests that LECT2 may be a multifunctional protein involved in cell growth, differentiation, damage/repair process and autoimmune response [3e6]. Proteins with sequence similarity to LECT2 have been identified in many vertebrates in addition to humans, such as carp (Cyprinus carpio) [7], rainbow trout (Oncorhynchus mykiss) [8], cattle (Bos taurus) [2], mouse (Mus musculus) [9], chicken (Gallus gallus) [10] and zebrafish (Danio rerio) [6]. In this paper, we report the molecular cloning of a full-length cDNA encoding pLECT2, the LECT2 from the liver of the fish croceine croaker (P. crocea), and the phylogenetic relationships of pLECT2 with the known homologues from other species.…”

“…The wide occurrence and similarity of LECT2 in different animals suggests that it has been quite strongly conserved and that it has an important role in the animals' life cycle. It was reported that human and bovine LECT2 gene was specifically expressed in the liver [2], however, the tissue expression profile of LECT2 gene shows that it is more generally distributed in fish tissues than in those of mammals [8]. Most recently, it was reported that the LECT2 mRNA of zebrafish liver was induced by up to 1000-fold upon infection by Aeromonas salmonicida and Staphylococcus aureus [6].…”

Induction of anti-viral genes during acute infection with Viral hemorrhagic septicemia virus (VHSV) genogroup IVa in Pacific herring (Clupea pallasii)

“…LECT2 has been identified in a number of fish species [37,45,46] and is often categorized as an acute phase protein (APP). LECT2 is highly up-regulated in the liver in response to bacterial infection [37,47].…”