Molecular Cloning of <i>PbSTKL1</i> Gene from <i>Plasmodiophora brassicae</i> Expressed during Clubroot Development

Ando, Sugihiro; Yamada, Takehiko; Asano, Takeshi; Kamachi, Shinichiro; Tsushima, Seiya; Hagio, Takashi; Tabei, Yutaka

doi:10.1111/j.1439-0434.2006.01078.x

Cited by 22 publications

(5 citation statements)

References 22 publications

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“…In contrast, the PbTPS1 gene of P. brassicae, which was also expressed in planta, was identified as a trehalose-6-phosphate synthase (TPS) gene, based on the high homology of the predicted protein sequence to other TPS proteins (Brodmann et al 2002). The molecular cloning of the PbSTKL1 gene from P. brassicae with homology to kinase revealed another gene with a possible function (Ando et al 2006b). Bulman et al (2006) isolated 76 ESTs of P. brassicae from a library for which they found some homologies to fungal genes.…”

Section: Short Introduction To the Life-cycle And Biology Of The Clubmentioning

confidence: 94%

What can we learn from clubroots: alterations in host roots and hormone homeostasis caused by Plasmodiophora brassicae

Ludwig‐Müller

Schuller

2008

Eur J Plant Pathol

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The clubroot disease of cruciferous crops is caused by an obligate biotrophic protist, Plasmodiophora brassicae. The disease is characterized by the development of large root galls accompanied by changes in source-sink relations and the hormonal balance within the plant. Since the disease is difficult to control, it is of high economic interest to understand the events leading to gall formation. In this review we will give an overview on the current knowledge of changes brought about in the host root by this obligate biotrophic pathogen. Emphasis will be on the regulation of changes in plant hormone homeostasis, mainly auxins and cytokinins; the possible role of secondary metabolites, especially indole glucosinolates, in gall formation and auxin homeostasis will be discussed. Also, results from mutant analysis and microarrays using the model plant Arabidopsis thaliana are presented.

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Section: Short Introduction To the Life-cycle And Biology Of The Clubmentioning

confidence: 94%

What can we learn from clubroots: alterations in host roots and hormone homeostasis caused by Plasmodiophora brassicae

Ludwig‐Müller

Schuller

2008

Eur J Plant Pathol

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“…The nucleotide sequence of the cloned PRO1 contains a large number of introns (eight within 1847 bp). Interestingly, the two previously reported P. brassicae genes, PbTPS1 (Brodmann et al ., 2002) and PbSTKL1 (Ando et al ., 2006), also contain one and 14 short introns, respectively. Bulman et al .…”

Section: Discussionmentioning

confidence: 99%

“…Up‐regulation of PRO1 expression suggests that Pro1 may be important in pathogenesis. To date, the expression profiles of P. brassicae genes correlated with particular stages of pathogenesis have been described in only a few studies (Ando et al ., 2006; Brodmann et al ., 2002; Ito et al ., 1999; Siemens et al ., 2009b), and the molecular function of most of these genes remains unknown.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of a serine protease Pro1 from Plasmodiophora brassicae that stimulates resting spore germination

Feng

Hwang

et al. 2010

Molecular Plant Pathology

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Clubroot, caused by Plasmodiophora brassicae, is one of the most serious diseases of cultivated cruciferous crops in the world. However, the basis for pathogenicity in P. brassicae is not well understood. In this study, a serine protease gene (PRO1) was cloned from P. brassicae and its molecular characteristics were investigated. Southern analysis and specific polymerase chain reaction (PCR) amplification indicated that PRO1 is a single-copy gene present in a broad range of P. brassicae pathotypes. Northern analysis revealed that the expression of PRO1 was induced during plant infection, and that the quantity of transcript fluctuated according to the stage of pathogenesis. Amino acid sequence analysis suggested that the encoded protein (Pro1) belongs to the S28 family of proteases, with a predicted signal peptide and a theoretical molecular mass of 49.4 kDa. The open reading frame (ORF) of PRO1 was transferred into Pichia pastoris and Pro1 was heterologously produced. Pro1 showed proteolytic activity on skimmed milk and N-succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin, and the activity could be inhibited by serine protease inhibitors and the chelating agent ethylenediaminetetraacetic acid. The optimal temperature of Pro1 was 25 degrees C, and it exhibited high activity at pH 6.0-6.4. These values coincide with the temperature and pH conditions favourable for P. brassicae resting spore germination in the field. When Pro1 was used to treat canola root exudates, it enhanced the stimulating effect of the root exudates on P. brassicae resting spore germination, indicating that Pro1 may play a role during clubroot pathogenesis by stimulating resting spore germination through its proteolytic activity.

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“…There are great differences in the biological and molecular characteristics of different pathogenic strains, which impede the research progress of pathogenesis. Up to now, only a few genes considering as the pathogenic factors in P. brassicae have been identified (Ando et al, 2006;Bulman et al, 2006;Feng et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptome Analysis Reveals Key Pathways and Hub Genes in Rapeseed During the Early Stage of Plasmodiophora brassicae Infection

Long

et al. 2020

Front. Genet.

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Rapeseed (Brassica napus L., AACC, 2n = 38) is one of the most important oil crops around the world. With intensified rapeseed cultivation, the incidence and severity of clubroot infected by Plasmodiophora brassicae Wor. (P. brassicae) has increased very fast, which seriously impedes the development of rapeseed industry. Therefore, it is very important and timely to investigate the mechanisms and genes regulating clubroot resistance (CR) in rapeseed. In this study, comparative transcriptome analysis was carried out on two rapeseed accessions of R-(resistant) and S-(susceptible) line. Three thousand one hundred seventy-one and 714 differentially expressed genes (DEGs) were detected in the R-and S-line compared with the control groups, respectively. The results indicated that the CR difference between the R-and S-line had already shown during the early stage of P. brassicae infection and the change of gene expression pattern of R-line exhibited a more intense defensive response than that of S-line. Moreover, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of 2,163 relative-DEGs, identified between the R-and S-line, revealed that genes participated in plant hormone signal transduction, fatty acid metabolism, and glucosinolate biosynthesis were involved in regulation of CR. Further, 12 hub genes were identified from all relative-DEGs with the help of weighted gene co-expression network analysis. Haplotype analysis indicated that the natural variations in the coding regions of some hub genes also made contributed to CR. This study not only provides valuable information for CR molecular mechanisms, but also has applied implications for CR breeding in rapeseed.

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Molecular Cloning of PbSTKL1 Gene from Plasmodiophora brassicae Expressed during Clubroot Development

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Cited by 22 publications

References 22 publications

What can we learn from clubroots: alterations in host roots and hormone homeostasis caused by Plasmodiophora brassicae

What can we learn from clubroots: alterations in host roots and hormone homeostasis caused by Plasmodiophora brassicae

Molecular characterization of a serine protease Pro1 from Plasmodiophora brassicae that stimulates resting spore germination

Comparative Transcriptome Analysis Reveals Key Pathways and Hub Genes in Rapeseed During the Early Stage of Plasmodiophora brassicae Infection

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