Molecular cloning of heat shock protein 10 (Hsp10) and 60 (Hsp60) cDNAs from Galeruca daurica (Coleoptera: Chrysomelidae) and their expression analysis

Abstract

Galeruca daurica (Joannis) is a new outbreak pest in the Inner Mongolia grasslands in northern China. Heat shock protein 10 and 60 (Hsp10 and Hsp60) genes of G. daurica, designated as GdHsp10 and GdHsp60, were cloned by rapid amplification of cDNA ends techniques. Sequence analysis showed that GdHsp10 and GdHsp60 encoded polypeptides of 104 and 573 amino acids, respectively. Sequence alignment and phylogenetic analysis clearly revealed that the amino acids of GdHsp10 and GdHsp60 had high homology and were clustered with other Hsp10 and Hsp60 genes in insects which are highly relative with G. daurica based on morphologic taxonomy. The mRNA expression analysis by real-time PCR revealed that GdHsp10 and GdHsp60 were expressed at all development stages and in all tissues examined, but expressed highest in eggs and in adults' abdomen; both heat and cold stresses could induce mRNA expression of GdHsp10 and GdHsp60 in the 2nd instar larvae; the two Hsp genes were expressed from high to low with the extension of treatment time in G. daurica eggs exposed to freezing point. Overall, our study provides useful information to understand temperature stress responses of Hsp60 and Hsp10 in G. daurica, and provides a basis to further study functions of Hsp60/Hsp10 relative to thermotolerance and cold hardiness mechanism.

Molecular characterization and expression of six heat shock protein genes in relation to development and temperature in Trichogramma chilonis

“…padi [ 39 , 47 ]. In this study, Tchsp10 and Tchsp60 levels decreased during development, which is consistent with the findings of report on Galeruca daurica (Coleoptera: Chrysomelidae) [ 50 ]. Moreover, Tchsp21 .…”

“…puparum appears at 36°C [ 23 ]. In this study, Tchsps were sensitive to high temperatures (32 and 40°C), which is consistent with the expression patterns of hsps observed in other species [ 23 , 48 , 50 ]. The expression of Tchsp10 , Tchsp21 .…”

“…It has been well reported that HSPs are involved in the development of insects [ 47 , 48 ]. However, the expression patterns of hsps during development vary in insects [ 49 , 50 ]. For example, the expression of hsp60 increases from the larval stage to the adult stage in Liriomyza sativa (Diptera: Agromyzidae), while it decreases from nymph to adult in R .…”

“…The upregulation of hsp10 and hsp60 has also been reported in Apostichopus japonicus (Echinodermata: Holothuroidea) and G . daurica [ 31 , 50 ]. HSP10 is considered the co-chaperone of HSP60 and interacts with HSP60 in the same heat shock pathway [ 33 , 54 ].…”

Trade-offs between sperm viability and immune protein expression in honey bee queens (Apis mellifera)

“…9 An immunosuppression function of HSP10 has not been demonstrated in invertebrates, and its function in insects, apart from its role as a chaperone, has received little attention. [44][45][46] Figure 2. Correlations of spermathecal fluid proteins with sperm viability.…”