Molecular Cloning of Genic Male-Sterility Genes and Their Applications for Plant Heterosis via Biotechnology-based Male-sterility Systems

Wan, Xiangyuan; Shu, Wu

doi:10.5772/intechopen.86976

Cited by 6 publications

(3 citation statements)

References 103 publications

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“…So far, more than 100 GMS genes have been cloned in plants, and most of them are investigated in model plants Arabidopsis and rice, while less than 20 GMS genes have been reported in maize (Wan et al ., 2019). Notably, most of the reported GMS genes are identified via the forward genetic approaches, such as map‐based cloning, T‐DNA or transposon tagging and MutMap methods (Wan and Wu, 2020), which are dependent on the existing GMS mutants. As the GMS genes often show anther‐specific or anther‐preferential expression patterns, it is reasonable to predict GMS genes by using anther transcriptomic analysis (i.e.…”

Section: Discussionmentioning

confidence: 99%

CRISPR/Cas9‐based discovery of maize transcription factors regulating male sterility and their functional conservation in plants

Jiang

et al. 2021

Plant Biotechnology Journal

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Summary Identifying genic male‐sterility (GMS) genes and elucidating their roles are important to unveil plant male reproduction and promote their application in crop breeding. However, compared with Arabidopsis and rice, relatively fewer maize GMS genes have been discovered and little is known about their regulatory pathways underlying anther and pollen development. Here, by sequencing and analysing anther transcriptomes at 11 developmental stages in maize B73, Zheng58 and M6007 inbred lines, 1100 transcription factor (TF) genes were identified to be stably differentially expressed among different developmental stages. Among them, 14 maize TF genes (9 types belonging to five TF families) were selected and performed CRISPR/Cas9‐mediated gene mutagenesis, and then, 12 genes in eight types, including ZmbHLH51, ZmbHLH122, ZmTGA9‐1/‐2/‐3, ZmTGA10, ZmMYB84, ZmMYB33‐1/‐2, ZmPHD11 and ZmLBD10/27, were identified as maize new GMS genes by using DNA sequencing, phenotypic and cytological analyses. Notably, ZmTGA9‐1/‐2/‐3 triple‐gene mutants and ZmMYB33‐1/‐2 double‐gene mutants displayed complete male sterility, but their double‐ or single‐gene mutants showed male fertility. Similarly, ZmLBD10/27 double‐gene mutant displayed partial male sterility with 32.18% of aborted pollen grains. In addition, ZmbHLH51 was transcriptionally activated by ZmbHLH122 and their proteins were physically interacted. Molecular markers co‐segregating with these GMS mutations were developed to facilitate their application in maize breeding. Finally, all 14‐type maize GMS TF genes identified here and reported previously were compared on functional conservation and diversification among maize, rice and Arabidopsis. These findings enrich GMS gene and mutant resources for deeply understanding the regulatory network underlying male fertility and for creating male‐sterility lines in maize.

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Section: Discussionmentioning

confidence: 99%

CRISPR/Cas9‐based discovery of maize transcription factors regulating male sterility and their functional conservation in plants

Jiang

et al. 2021

Plant Biotechnology Journal

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“…In maize, the proportion of tissue-specific transcribed genes in anther is the highest (6.0%) when compared with other tissues during various developmental stages (Figure S6), suggesting that maize anther transcriptome has a special content and that the GRN underlying anther development may be more complicated. Previous studies have identified a number of functional genes involved in anther development, including TF-coding genes that are crucial regulatory nodes in the networks, enzyme-coding genes that participate in lipid and carbohydrate metabolism, and transporter genes [2,5,6,7,64,65]. Additionally, several miRNAs combined with their target genes were found to have important functions in regulating the floral organ identity and the subsequent development of anther and pollen [8,9].…”

Section: Discussionmentioning

confidence: 99%

Discovering and Constructing ceRNA-miRNA-Target Gene Regulatory Networks during Anther Development in Maize

Zhu

et al. 2019

IJMS

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The “competing endogenous RNA (ceRNA) hypothesis” has recently been proposed for a new type of gene regulatory model in many organisms. Anther development is a crucial biological process in plant reproduction, and its gene regulatory network (GRN) has been gradually revealed during the past two decades. However, it is still unknown whether ceRNAs contribute to anther development and sexual reproduction in plants. We performed RNA and small RNA sequencing of anther tissues sampled at three developmental stages in two maize lines. A total of 28,233 stably transcribed loci, 61 known and 51 potentially novel microRNAs (miRNAs) were identified from the transcriptomes. Predicted ceRNAs and target genes were found to conserve in sequences of recognition sites where their corresponding miRNAs bound. We then reconstructed 79 ceRNA-miRNA-target gene regulatory networks consisting of 51 known miRNAs, 28 potentially novel miRNAs, 619 ceRNA-miRNA pairs, and 869 miRNA-target gene pairs. More than half of the regulation pairs showed significant negative correlations at transcriptional levels. Several well-studied miRNA-target gene pairs associated with plant flower development were located in some networks, including miR156-SPL, miR159-MYB, miR160-ARF, miR164-NAC, miR172-AP2, and miR319-TCP pairs. Six target genes in the networks were found to be orthologs of functionally confirmed genes participating in anther development in plants. Our results provide an insight that the ceRNA-miRNA-target gene regulatory networks likely contribute to anther development in maize. Further functional studies on a number of ceRNAs, miRNAs, and target genes will facilitate our deep understanding on mechanisms of anther development and sexual plants reproduction.

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“…Notably, the 101 DEGs includes 21 key genes that are homologs of FATB, KASI, KASII, SAD, KCS, LACS, HCD, CER1, KCR, and OFADH in Arabidopsis, indicating that they are essential for anther cuticle and pollen exine biosynthesis (Figure 2; Supplemental Table 3) (Kunst and Samuels, 2009;Li-Beisson et al, 2013). Therefore, elucidating the functions of the predicted 101 novel lipid metabolic genes and confirming that they are GMS genes in maize using a reverse genetic approach will greatly improve our in-depth understanding of the molecular and biochemical networks regulating anther and pollen development in maize and other plants (Wan and Wu, 2020). This interesting work is ongoing in our lab.…”

Section: Molecular Plantmentioning

confidence: 99%

Lipid Metabolism: Critical Roles in Male Fertility and Other Aspects of Reproductive Development in Plants

et al. 2020

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Fatty acids and their derivatives are essential building blocks for anther cuticle and pollen wall formation. Disruption of lipid metabolism during anther and pollen development often leads to genic male sterility (GMS). To date, many lipid metabolism-related GMS genes that are involved in the formation of anther cuticle, pollen wall, and subcellular organelle membranes in anther wall layers have been identified and characterized. In this review, we summarize recent progress on characterizing lipid metabolism-related genes and their roles in male fertility and other aspects of reproductive development in plants. On the basis of cloned GMS genes controlling biosynthesis and transport of anther cutin, wax, sporopollenin, and tryphine in Arabidopsis, rice, and maize as well as other plant species, updated lipid metabolic networks underlying anther cuticle development and pollen wall formation were proposed. Through bioinformatics analysis of anther RNA-sequencing datasets from three maize inbred lines (Oh43, W23, and B73), a total of 125 novel lipid metabolism-related genes putatively involved in male fertility in maize were deduced. More, we discuss the pathways regulating lipid metabolism-related GMS genes at the transcriptional and post-transcriptional levels. Finally, we highlight recent findings on lipid metabolism-related genes and their roles in other aspects of plant reproductive development. A comprehensive understanding of lipid metabolism, genes involved, and their roles in plant reproductive development will facilitate the application of lipid metabolism-related genes in gene editing, haploid and callus induction, molecular breeding and hybrid seed production in crops.

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Molecular Cloning of Genic Male-Sterility Genes and Their Applications for Plant Heterosis via Biotechnology-based Male-sterility Systems

Cited by 6 publications

References 103 publications

CRISPR/Cas9‐based discovery of maize transcription factors regulating male sterility and their functional conservation in plants

CRISPR/Cas9‐based discovery of maize transcription factors regulating male sterility and their functional conservation in plants

Discovering and Constructing ceRNA-miRNA-Target Gene Regulatory Networks during Anther Development in Maize

Lipid Metabolism: Critical Roles in Male Fertility and Other Aspects of Reproductive Development in Plants

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