Molecular Cloning of Equine (Thoroughbred) Myostatin cDNA and Detection of Myostatin Precursor Proteins in the Serum.

Hosoyama, Tohru; Kawada, Shigeo; Oshiumi, Ryosuke; Yoneda, Sachie; Soeta, Chie; Yamanouchi, Keitaro; Hasegawa, Telhisa; Ishida, Nobushige; Mukoyama, Harutaka; Ishii, Naokata; Tachi, Chikashi

doi:10.1262/jrd.48.335

Cited by 9 publications

(9 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are few studies in the horse and to date, only one report has identified myostatin precursor and mature myostatin protein expression in the skeletal muscles ( Semitendinosus, Semimembranosus, Splenius, Gluteus medius) of Thoroughbred and Kiso-uma breeds of horses [19]. That gene and protein expression for myostatin and its receptor were largely exclusive to skeletal muscle, suggested that as reported for other species, myostatin may have an important role in equine muscle function.…”

Section: Discussionmentioning

confidence: 95%

Post-Mortem Stability of RNA in Skeletal Muscle and Adipose Tissue and the Tissue-Specific Expression of Myostatin, Perilipin and Associated Factors in the Horse

Morrison

Chen

Harris³

et al. 2014

PLoS ONE

View full text Add to dashboard Cite

Obesity, a major concern for equine welfare, is highly prevalent in the leisure horse population. Skeletal-muscle and adipose tissues are important determinants of maintenance energy requirements. The myostatin and perilipin pathways play key roles in the regulation of muscle mass and lipolysis respectively and have both been associated with obesity predisposition in other mammalian species. High quality samples, suitable for molecular biology, are an essential prerequisite for detailed investigations of gene and protein expression. Hence, this study has evaluated a) the post-mortem stability of RNA extracted from skeletal-muscle and adipose-tissues collected under commercial conditions and b) the tissue-specific presence of myostatin, the moystatin receptor (activin receptor IIB, ActRIIB), follistatin and perilipin, genes and proteins across a range of equine tissues. Objectives were addressed using tissues from 7 Thoroughbred horses presented for slaughter at a commercial abattoir; a) samples were collected at 7 time-points from Masseter muscle and perirenal adipose from 5 minutes to 6 hours post-mortem. Extracted RN was appraised by Optical Density analysis and agarose-gel electrophoresis. b) Quantitative real time PCR and Western Blotting were used to evaluate gene and protein expression in anatomically-defined samples collected from 17 tissues (6 organs, 4 skeletal muscles and 7 discrete adipose depots). The results indicate that, under the present collection conditions, intact, good quality RNA could be extracted from skeletal-muscle for up to 2 hours post-mortem. However, RNA from adipose tissue may be more susceptible to degradation/contamination and samples should be collected no later than 30 minutes post-mortem. The data also show that myostatin and ActRIIB genes and proteins were almost exclusively expressed in skeletal muscle. The follistatin gene showed a more diverse gene expression profile, with expression evident in several organs, adipose tissue depots and skeletal muscles. Perilipin gene and protein were almost exclusively expressed by adipose tissue.

show abstract

Section: Discussionmentioning

confidence: 95%

Post-Mortem Stability of RNA in Skeletal Muscle and Adipose Tissue and the Tissue-Specific Expression of Myostatin, Perilipin and Associated Factors in the Horse

Morrison

Chen

Harris³

et al. 2014

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…Hosoyama et al . () isolated and sequenced MSTN cDNA from a Thoroughbred horse that was mapped to chromosome 18. Mutations in the equine MSTN have been identified and are associated with racing phenotypes influencing racing performance and muscle fibre proportions (Petersen et al .…”

Section: Myostatin Polymorphisms In Horsementioning

confidence: 99%

Themyostatingene: an overview of mechanisms of action and its relevance to livestock animals

2018

View full text Add to dashboard Cite

Myostatin, also known as growth differentiation factor 8, a member of the transforming growth factor-beta super-family, is a negative regulator of muscle development. Myostatin acts at key points during pre- and post-natal life of amniotes that ultimately determine the overall muscle mass of an animal. Mutations have already demonstrated the impact of attenuating myostatin activity on muscle development. A number of large animals, including cattle, sheep, dogs and humans, display the 'double muscled' phenotype due to mutations in the myostatin gene. Here, we firstly give an overview of the molecular pathways regulated by myostatin that control muscle development. Then we describe the natural mutations and their associated phenotypes as well as the physiological influence of altering myostatin expression in livestock animals (cattle, sheep, goat, horse, pig, rabbit and chicken). Knowledge of null alleles and polymorphisms in the myostatin gene are of great interest in the animal breeding field, and it could be utilized to improve meat production in livestock animals.

show abstract

“…skeletal muscle [1], it has been reported that myostatin is secreted into the circulation [13][14][15][16]. Some investigators have shown that circulating levels of myostatin-like protein are increased in HIV-infected men and elderly persons with weight loss and muscle wasting symptoms [13,17].…”

mentioning

confidence: 99%

Role of Serum Myostatin during the Lactation Period

Hosoyama

Yamanouchi

Nishihara

2006

Journal of Reproduction and Development

Self Cite

View full text Add to dashboard Cite

Abstract. Myostatin, also known as GDF-8 (Growth/Differentiation Factor-8), is a member of the TGF-β superfamily that negatively regulates skeletal muscle mass in mammals. Mutation of the myostatin gene in mice, cattle, and humans causes a massively developed skeletal muscle, characterized by muscle hypertrophy and hyperplasia. Although myostatin is predominantly expressed in skeletal muscle tissue, several recent studies have shown the presence of myostatin protein in blood and suggested a possible role for circulating myostatin in the regulation of skeletal muscle mass. In the present study, we examined changes in the levels of active form myostatin (13 kDa) in serum after birth by Western blot analysis to predict the role of serum myostatin in early postnatal muscle growth in the rat. Interestingly, the amount of active form myostatin in serum increased after birth and then decreased along with ageing after weaning. To clarify the role of increased serum myostatin during the postnatal period, we administrated follistatin, an inhibitor of myostatin activity, into postnatal rats intraperitoneally just after birth. Follistatin-administration during the postnatal period caused selective hypertrophy of type II muscle fibers in the soleus muscle. These results demonstrate that myostatin in serum acts on skeletal muscle and negatively regulates early postnatal muscle growth.

show abstract

Molecular Cloning of Equine (Thoroughbred) Myostatin cDNA and Detection of Myostatin Precursor Proteins in the Serum.

Cited by 9 publications

References 16 publications

Post-Mortem Stability of RNA in Skeletal Muscle and Adipose Tissue and the Tissue-Specific Expression of Myostatin, Perilipin and Associated Factors in the Horse

Post-Mortem Stability of RNA in Skeletal Muscle and Adipose Tissue and the Tissue-Specific Expression of Myostatin, Perilipin and Associated Factors in the Horse

Themyostatingene: an overview of mechanisms of action and its relevance to livestock animals

Role of Serum Myostatin during the Lactation Period

Contact Info

Product

Resources

About