Molecular cloning of CYP1A gene and its expression by benzo(a)pyrene from goldfish (<i>Carassius auratus</i>)

Oh, Seung-Min; Ryu, Byung Taek; Kim, Ha Ryong; Choi, Kwanghoon; Chung, Kyu-Hyuck

doi:10.1002/tox.20422

Cited by 19 publications

(6 citation statements)

References 66 publications

(63 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…And there is yet the need to provide conclusive evidence that (or which) CYP isoforms are able to metabolize acrylamide in fish. Still, caution is mandatory here, as CYP1A in fish is known to be induced by several classes of environmental toxicants (Bucheli and Font, ; Buhler and Wang‐Buhler, ; Haasch et al ., ; Oh et al ., ), including non CYP activatable (e.g. Diniz et al ., ; Olsvik et al ., ).…”

Section: Discussionmentioning

confidence: 99%

Histopathological findings on Carassius auratus hepatopancreas upon exposure to acrylamide: correlation with genotoxicity and metabolic alterations

Larguinho

Costa

Sousa

et al. 2013

J of Applied Toxicology

View full text Add to dashboard Cite

Acrylamide is an amide used in several industrial applications making it easily discharged to aquatic ecosystems. The toxicity of acrylamide to aquatic organisms is scarcely known, although previous studies with murine models provided evidence for deleterious effects. To assess the effects of acrylamide to freshwater fish, goldfish (Carassius auratus L.) were exposed to several concentrations of waterborne acrylamide and analysed for genotoxic damage, alterations to detoxifying enzymes and histopathology. Results revealed a dose-dependent increase in total DNA strand breakage, the formation of erythrocytic nuclear abnormalities and in the levels of hepatic cytochrome P4501A (CYP1A) and glutathione S-transferase (GST) activity. In addition, acrylamide induced more histopathological changes to pancreatic acini than to the hepatic parenchyma, regardless of exposure concentration, whereas hepatic tissue only endured significant alterations at higher concentrations of exposure. Thus, results confirm the genotoxic potential of acrylamide to fish and its ability to induce CYP1A, probably as a direct primary defence mechanism. This strongly suggests the substance's pro-mutagenic potential in fish, similarly to what is known for rodents. However, the deleterious effects observed in the pancreatic acini, more severe than in the liver, could indicate a specific, albeit unknown toxic mechanism of acrylamide to fish that overran the organism's metabolic defences against a chemical agent rather than causing a general systemic failure.

show abstract

Section: Discussionmentioning

confidence: 99%

Histopathological findings on Carassius auratus hepatopancreas upon exposure to acrylamide: correlation with genotoxicity and metabolic alterations

Larguinho

Costa

Sousa

et al. 2013

J of Applied Toxicology

View full text Add to dashboard Cite

show abstract

“…Hepatic CYP1A1 and CYP1A2 are expressed at the highest levels in pre-pubertal male rats compared to adult rats [44]. CYP1A1 is expressed in human testes [45] and B[a]P significantly induced the expression of CYP1A1 in multiple tissues including the gonads in goldfish ( Carassius auratus ) [46]. It is not known if CYP1A1 is expressed at a greater level in spermatogonial stem cells of pre-pubertal animals, but greater expression would result theoretically in greater bioactivation of B[a]P. Consequently, more DNA damage could be realized in pre-pubertal spermatogonial cells.…”

Section: Discussionmentioning

confidence: 99%

Early-Life Exposure to Benzo[a]pyrene Increases Mutant Frequency in Spermatogenic Cells in Adulthood

McMahan

Walter

2014

PLoS ONE

View full text Add to dashboard Cite

Children are vulnerable to environmental mutagens, and the developing germline could also be affected. However, little is known about whether exposure to environmental mutagens in childhood will result in increased germline mutations in subsequent adult life. In the present study, male transgenic lacI mice at different ages (7, 25 and 60 days old) were treated with a known environmental mutagen (benzo[a]pyrene, B[a]P) at different doses (0, 50, 200 or 300 mg/kg body weight). Mutant frequency was then determined in a meiotic cell type (pachytene spermatocyte), a post-meiotic cell type (round spermatid) and epididymal spermatozoa after at least one cycle of spermatogenesis. Our results show that 1) mice treated with B[a]P at 7 or 25 days old, both being pre-adult ages, had significantly increased mutant frequencies in all spermatogenic cell types tested when they were 60 days old; 2) spermatogenic cells from mice treated before puberty were more susceptible to B[a]P-associated mutagenesis compared to adult mice; and 3) unexpectedly, epididymal spermatozoa had the highest mutant frequency among the spermatogenic cell types tested. These data show that pre-adult exposure to B[a]P increases the male germline mutant frequency in young adulthood. The data demonstrate that exposure to environmental genotoxins at different life phases (e.g., pre-adult and adult) can have differential effects on reproductive health.

show abstract

“…Student's t ‐tests were used to detect significant effects of BaP between exposure and control groups (Oh et al, ). Pearson's coefficients were used to examine correlations among responses.…”

Section: Methodsmentioning

confidence: 99%

Cytochrome P450 system expression and DNA adduct formation in the liver of Zacco platypus following waterborne Benzo(a)pyrene exposure: implications for biomarker determination

Lee

Kim

Yoon

et al. 2012

Environmental Toxicology

View full text Add to dashboard Cite

Benzo(a)pyrene (BaP) is a polycyclic aromatic hydrocarbon that causes mutations and tumor formation. Zacco platypus is a sentinel species that is suitable for monitoring aquatic environments. We studied cytochrome P450 system (CYP system) expression and DNA adduct formation in the liver of Z. platypus following waterborne exposure to BaP. The results showed both dose and time dependency. The significant induction levels of CYP system mRNA and protein reached maximums at 2 days and 14 days, respectively, and hepatosomatic index was maximally induced at 4 days during 14 days BaP exposure. DNA adduct formation was significantly induced compared to corresponding controls (t-test, p < 0.01) after 4 days of exposure in 100 μg/L BaP. These results indicate that the only use of mRNA expression level of CYP system as a biomarker make us underestimate prolonged toxicity (4-14 days) of BaP and the only use of protein expression level of CYP system make us underestimate acute toxicity (1-2 days) of BaP. Therefore, we suggests that a combinational use of the mRNA expression level and protein expression level of CYP system, hepatosomatic index is a useful biomarker in risk assessment of waterborne BaP exposure. In addition, DNA adduct formation was a useful biomarker in risk assessment of waterborne BaP exposure at 4 days. CYP1A was a more sensitive biomarker than CYP reductase for BaP exposure when considering both the mRNA and protein level. Furthermore, our results show that Z. platypus is a useful species for assessing the risk of waterborne BaP exposure.

show abstract

Molecular cloning of CYP1A gene and its expression by benzo(a)pyrene from goldfish (Carassius auratus)

Cited by 19 publications

References 66 publications

Histopathological findings on Carassius auratus hepatopancreas upon exposure to acrylamide: correlation with genotoxicity and metabolic alterations

Histopathological findings on Carassius auratus hepatopancreas upon exposure to acrylamide: correlation with genotoxicity and metabolic alterations

Early-Life Exposure to Benzo[a]pyrene Increases Mutant Frequency in Spermatogenic Cells in Adulthood

Cytochrome P450 system expression and DNA adduct formation in the liver of Zacco platypus following waterborne Benzo(a)pyrene exposure: implications for biomarker determination

Contact Info

Product

Resources

About