Molecular cloning of Bacillus subtilis genes involved in DNA metabolism

Abstract

Different clones carrying a chromosomal DNA fragment able to transform Bacillus subtilis mutants dnaA13, dnaB19, dnaG5, recG40 and polA42 to a wild-type phenotype were isolated from a library constructed in plasmid pJH101. A lambda recombinant clone carrying a chromosomal fragment able to transform dnaC mutants was obtained from a lambda Charon 4A library. A restriction map of the cloned DNA fragments was constructed. The 11.3 kb cloned DNA fragment of plasmid pMP60-13 containing the wild-type sequence of dnaG5 was shown to transform a recF33 mutant as well.

Genetic Recombination in Bacillus subtilis 168: Effects of recU and recS Mutations on DNA Repair and Homologous Recombination

“…L47648.Gb_Ba) (27). Furthermore, a B. subtilis region (plasmid pMP29-42) able to rescue the recG40 mutation (24) has been sequenced. The pMP29-42-borne DNA segment bears two truncated ORFs, termed "yppB" and "yppC" (27) (accession no.…”

The Bacillus subtilis genes for ribonucleotide reductase are similar to the genes for the second class I NrdE/NrdF enzymes of Enterobacteriaceae

“…In Bacilltls stlbti1i.r the isolation and characterization of a temperature-sensitive mutant (ts-A 73) led to the identification of the nrd locus encoding a RR, functionally similar to the aerobic class I RR of E. coli (Bazill & Karamata, 1972). In a marker rescue screening, a clone positive for the ts-A 13 allele (previously named dnaA-73) was isolated from a plasmid bank of B. stlbtilis genomic DNA (Perego et al, 1987). The nrd (dnaA) locus was mapped at about 167' on the chromosome, between theglnRA operon and thepks locus (Fisher etal., 1984;Gianni & Galizzi, 1986;Scotti et al, 1993).…”

“…Plasmid clone pMP25.8 (Perego et al, 1987) was able to marker rescue the temperature-sensitive mutant ts-A 73 in transformation experiments and direct selection to ts'. None of the transformants was CmR.…”

In vivo relations between pAMβ1‐encoded type I topoisomerase and plasmid replication

“…The strains used were B. subtilis SB202 (Bruand et al ., 1991), 1A224 and its isogenic polA5 mutant 1A226 (Perego et al ., 1987) and E. coli TG1 and IC1806 (Aleixandre and Blanco, 1987). The strain SBTOPO containing the topβ gene in its chromosome was constructed as follows.…”