Molecular cloning of aromatic degradative genes from Pseudomonas stutzeri

GARCIAVALDES, E

doi:10.1016/0378-1097(89)90215-2

Cited by 3 publications

(3 citation statements)

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“…stutzeri of special interest in ecological studies. This species has gained interest because of its metabolic properties, such as degradation of environmental pollutants (Baggi et al 1987 ;García-Valdés et al 1989 ;Rosselló-Mora et al 1994b) and high-molecular-weight polyethylene glycols (Obradors and Aguilar 1991). Furthermore, Ps.…”

Section: Introductionmentioning

confidence: 99%

Genetic relationships amongPseudomonas stutzeristrains based on molecular typing methods

Bennasar

Guasp

Tesar

et al. 1998

Journal of Applied Microbiology

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A . B EN N AS AR , C. GU A SP , M . TE SA R AN D J . LA LU C AT . 1998. Detailed characterization of the genetic variability among strains belonging to Pseudomonas stutzeri was achieved using different rapid molecular typing methods based on polymerase chain reaction (PCR), Southern blot and Western blot. Consensus motifs complementary to fragments of repetitive elements dispersed throughout the genomes of bacteria were used as primers and allowed differentiation at subspecies levels. Further and simple differentiation was also achieved based on the direct amplification of spacer regions between 16S and 23S rRNA, combined with single-strand conformation polymorphism (SSCP) analysis of the generated fragments. These methods are fast, sensitive, reliable for determining relationships, and have demonstrated a great genetic diversity among the strains of Ps. stutzeri studied in agreement with the heterogeneous phenotypic traits of the species.

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Section: Introductionmentioning

confidence: 99%

Genetic relationships amongPseudomonas stutzeristrains based on molecular typing methods

Bennasar

Guasp

Tesar

et al. 1998

Journal of Applied Microbiology

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“…A partial chromosomally encoded naphthalene degradation pathway has been reported in P. putida PMD-1 (59) in which only salicylate degradation (the lower pathway) was plasmid encoded. In P. stutzeri AN10 and AN11, no plasmid could be associated with this metabolic process (20,21).…”

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“…Recovery of DNA from agarose was performed by the Geneclean method (Geneclean kit; Bio 101, Inc., La Jolla, Calif.). Probes for nahG and nahH were obtained from P. stutzeri AN 11 as a 2-kb PstI fragment and 1.6-kb PstI-XhoI fragment of plasmid pLIBI 13 (20). A probe for nahA, from plasmid NAH7, was obtained as a 1.5-kb SmaI-PstI fragment of pKGX584 (41).…”

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Comparative biochemical and genetic analysis of naphthalene degradation among Pseudomonas stutzeri strains

Rosselló‐Móra

Lalucat

Garcı́a-Valdés

1994

Appl Environ Microbiol

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Of a 49-strain collection of Pseudomonas stutzeri species, 11 isolates were able to degrade naphthalene and 1 isolate was able to use m-and p-toluate as sole carbon and energy sources. Of these 12 strains, 10 shared a highly homologous set of naphthalene catabolic genes, even though they belong to four different genomovars. These genes differed from those present in plasmid NAH7. In only one of these degraders could a plasmid-encoded pathway be demonstrated, and a chromosome-encoded pathway is proposed for the remaining strains. meta cleavage of catechol was only observed in those strains able to metabolize alkyl derivatives of catechol.

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A ribosomal RNA operon from Pseudomonas stutzeri Zobell

Kerkhof

1997

Gene

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Molecular cloning of aromatic degradative genes from Pseudomonas stutzeri

Cited by 3 publications

References 5 publications

Genetic relationships amongPseudomonas stutzeristrains based on molecular typing methods

Genetic relationships amongPseudomonas stutzeristrains based on molecular typing methods

Comparative biochemical and genetic analysis of naphthalene degradation among Pseudomonas stutzeri strains

A ribosomal RNA operon from Pseudomonas stutzeri Zobell

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