Molecular cloning of an oxytocin-like receptor expressed in the chicken shell gland

Gubrij, Konstantin I.; Chaturvedi, Chandra Mohini; Ali, Nawab; Cornett, Lawrence E.; Kirby, John D.; Wilkerson, James A.; Mikhailova, Marina V.; Turner, Michael L.; Baeyens, Dennis A.

doi:10.1016/j.cbpc.2005.05.011

Cited by 38 publications

(17 citation statements)

References 33 publications

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“…So far, three AVT receptor subtypes termed VT1, VT2, and VT3 have been revealed and characterized in the domestic chicken (Tan et al 2000;Cornett et al 2003;Gubrij et al 2005), although only the VT2 receptor (VT2R) has been found to be highly expressed in the pituitary gland (Cornett et al 2003;Jurkevich et al 2005). The amino acid sequence of the VT2R protein matches largely the mammalian V1b receptor sequence.…”

Section: Introductionmentioning

confidence: 90%

Immunohistochemical characterization of chicken pituitary cells containing the vasotocin VT2 receptor

et al. 2008

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Research in mammals has demonstrated a variety of regulatory effects of vasopressin and oxytocin on endocrine functions of the anterior pituitary gland. Less evidence is available regarding the hypophysiotropic action of arginine vasotocin (AVT) comprising vasopressic and oxytocic activities in birds. Some hypophysiotropic effects of AVT may result from its interactions with brain circuits controlling pituitary functions, whereas others are caused by its direct affect on pituitary cells. Use of an antiserum to the vasotocin receptor VT2 (VT2R) has revealed numerous immunoreactive cells in the anterior pituitary gland of the chicken. The objective of the present study has been to identify endocrine phenotypes of chicken pituitary cells containing VT2R by means of immunohistochemical labeling. VT2R immunoreactivity has been found in all cells immunoreactive for adrenocorticotropin and alpha-melanotropin. Approximately 10% of labeled lactotropes are also immunoreactive for VT2R and lie around the anatomical boundary dividing the cephalic and caudal lobes. In both corticotropes/melanotropes and lactotropes, immunoreactive VT2R is present in a narrow layer outlining cell bodies. Immunoreactive VT2R is not found in gonadotropes, thyrotropes, or somatotropes. These results provide evidence for the important role of VT2Rs in mediating effects of AVT on endocrine secretion from corticotropes and, partially, from lactotropes.

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Section: Introductionmentioning

confidence: 90%

Immunohistochemical characterization of chicken pituitary cells containing the vasotocin VT2 receptor

et al. 2008

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“…The avian VT4R is orthologous to the V1AR ). The MTR is the ortholog of the OTR (Gubrij et al 2005). In the Japanese tree frog Hyla japonica, Kohno et al (2003) isolated a single VT receptor (V2-like) cDNA.…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning, sequencing and phylogeny of vasotocin receptor genes in the air-breathing catfish Heteropneustes fossilis with sex dimorphic and seasonal variations in tissue expression

Rawat

Chaube

Joy

2015

Fish Physiol Biochem

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Vasotocin (VT) is the ortholog of vasopressin (VP) in non-mammalian vertebrates and is known for multiple functions. Teleost fishes have a complete repertoire of known VP/VT receptor subtypes (vasopressin type, VR): two V1A subtypes (V1Aa and V1Ab or V1a1 and V1a2) and five V2 subtypes (V2A1, V1A2, V2B1, V2B2 and V2C). Full-length cDNAs of v1a1, v1a2 and v2 (v2a1) with ORFs of 1,308, 1,137 and 1,527 bp, respectively, were cloned and characterized in the catfish Heteropneustes fossilis (Siluriformes, Ostariophysi). BLAST analysis revealed that the genes encoded three VT receptors, V1a1, V1a2 and V2 of 436, 379 and 509 amino acid residues, respectively. The predicted proteins showed typical features of the seven-transmembrane domain receptor core structure with hallmark triplets Asp-Arg-Tyr/Asp-Arg-His (DRY/DRH) and the variable intracellular loop III of vertebrate neurohypophysial hormone receptors. Phylogenetic analysis of the deduced protein sequences revealed that they clustered with the V1Aa, V1Ab and V2A1, respectively, of other teleosts. The V2R has a sequence identity of 70-76% with V2A1 than with the V2B type (sequence identity 43-49%). Semiquantitative PCR analysis showed that the receptor gene transcripts were expressed ubiquitously in the tissues examined (brain, pituitary, gonads, liver, muscle, kidney and gills) and displayed sex and seasonal fluctuations in a tissue-specific manner. The results form a basis for functional studies on the VT receptors in the catfish.

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“…MT receptors have also been characterized and sequenced in amphibians(Akhundova and others, 1996; Kohno and others, 2003; Acharjee and others, 2004; Searcy and others, 2011). In birds, there are four nonapeptide receptors VT1-VT4(Tan and others, 2000; Gubrij and others, 2005; Leung and others, 2011). The VT4, VT2, VT1, and VT3 avian receptors appear to be homologues of the mammalian V1a, V1b, V2 and OT receptors, respectively.…”

Section: Introductionmentioning

confidence: 99%

Species, sex and individual differences in the vasotocin/vasopressin system: Relationship to neurochemical signaling in the social behavior neural network

Albers

2015

Frontiers in Neuroendocrinology

165

152

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Arginine-vasotocin(AVT)/arginine vasopressin (AVP) are members of the AVP/oxytocin (OT) superfamily of peptides that are involved in the regulation of social behavior, social cognition and emotion. Comparative studies have revealed that AVT/AVP and their receptors are found throughout the “Social Behavior Neural Network” and display the properties expected from a signaling system that controls social behavior (i.e., species, sex and individual differences and modulation by gonadal hormones and social factors). Neurochemical signaling within the SBNN likely involves a complex combination of synaptic mechanisms that co-release multiple chemical signals (e.g., classical neurotransmitters and AVT/AVP as well as other peptides) and non-synaptic mechanisms (i.e., volume transmission). Crosstalk between AVP/OT peptides and receptors within the SBNN is likely. A better understanding of the functional properties of neurochemical signaling in the SBNN will allow for a more refined examination of the relationships between this peptide system and species, sex and individual differences in sociality.

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Molecular cloning of an oxytocin-like receptor expressed in the chicken shell gland

Cited by 38 publications

References 33 publications

Immunohistochemical characterization of chicken pituitary cells containing the vasotocin VT2 receptor

Immunohistochemical characterization of chicken pituitary cells containing the vasotocin VT2 receptor

Molecular cloning, sequencing and phylogeny of vasotocin receptor genes in the air-breathing catfish Heteropneustes fossilis with sex dimorphic and seasonal variations in tissue expression

Species, sex and individual differences in the vasotocin/vasopressin system: Relationship to neurochemical signaling in the social behavior neural network

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