Molecular cloning of alcohol dehydrogenase genes of the yeastPichia stipitis and identification of the fermentative ADH

Passoth, Volkmar; Schäfer, Bernd; Liebel, Birgit; Weierstall, Thomas; Klinner, Ulrich

doi:10.1002/(sici)1097-0061(1998100)14:14<1311::aid-yea315>3.0.co;2-t

Cited by 41 publications

(7 citation statements)

References 54 publications

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“…It is possible that in D. bruxellensis , the same ADH gene is responsible for both production and consumption of ethanol, which would explain its upregulation when the glucose is almost finished. Similar observations have been made in Scheffersomyces stipitis and Wickerhamomyces anomalus .…”

Section: Discussionsupporting

confidence: 86%

Adaptation of Dekkera bruxellensis to lignocellulose‐based substrate

Tiukova

Pita

Sundell

et al. 2014

Biotech and App Biochem

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Adaptation of Dekkera bruxellensis to lignocellulose hydrolysate was investigated. Cells of D. bruxellensis were grown for 72 and 192 H in batch and continuous culture, respectively (adapted cells). Cultivations in semisynthetic medium were run as controls (nonadapted cells). To test the adaptation, cells from these cultures were reinoculated in the lignocellulose medium, and growth and ethanol production characteristics were monitored. Cells adapted to lignocellulose hydrolysate had a shorter lag phase, grew faster, and produced a higher ethanol concentration as compared with nonadapted cells. A stability test showed that after cultivation in rich medium, cells partially lost the adapted phenotype but still showed faster growth and higher ethanol production as compared with nonadapted cells. Because alcohol dehydrogenase genes have been described to be involved in the adaptation to furfural in Saccharomyces cerevisiae, an analogous mechanism of adaptation to lignocelluloses hydrolysate of D. bruxellensis was hypothesized. However, gene expression analysis showed that genes homologous to S. cerevisiae ADH1 were not involved in the adaptation to lignocelluloses hydrolysate in D. bruxellensis.

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Section: Discussionsupporting

confidence: 86%

Adaptation of Dekkera bruxellensis to lignocellulose‐based substrate

Tiukova

Pita

Sundell

et al. 2014

Biotech and App Biochem

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“…A number of ADH genes and their products from various yeasts have been characterized to date. [1][2][3][4] They similarly possess two Adhs located in the mitochondria, but little is known about their physiological functions in the compartments.…”

mentioning

confidence: 99%

The Crucial Role of Alcohol Dehydrogenase Adh3 inKluyveromyces marxianusMitochondrial Metabolism

Lertwattanasakul

Shigemoto

Rodrussamee

et al. 2009

Bioscience, Biotechnology, and Biochemistry

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“…While in P. stipitis, the function of some ADH enzymes are better understood, in particular PsADH1 and PsADH2 [13,17,44,[46][47][48][49]. Transcriptomic studies of the P. stipites adh system indicated that the PsADH activities are correlated with and induced under O 2 limited/microaerobic conditions [46,48].…”

Section: Future Perspective For Engineering New Strains For Better Bimentioning

confidence: 99%

“…The levels of PsADH1 and PsADH2 transcripts were observed, however, to be low through xylose metabolism relative to the transcript levels of other fermentative and glycolytic enzymes [13,17]. In addition, PsADH1 and PsADH2 were able to complement the growth of the S. cerevisiae ∆adh mutant on ethanol as a sole carbon source [47]. Moreover, PsADH3 to PsADH7 were speculated to keep the balance between the cofactors NADPH and NADH [17].…”

Section: Future Perspective For Engineering New Strains For Better Bimentioning

confidence: 99%

The Xylose Metabolizing Yeast Spathaspora passalidarum is a Promising Genetic Treasure for Improving Bioethanol Production

2020

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Currently, the fermentation technology for recycling agriculture waste for generation of alternative renewable biofuels is getting more and more attention because of the environmental merits of biofuels for decreasing the rapid rise of greenhouse gas effects compared to petrochemical, keeping in mind the increase of petrol cost and the exhaustion of limited petroleum resources. One of widely used biofuels is bioethanol, and the use of yeasts for commercial fermentation of cellulosic and hemicellulosic agricultural biomasses is one of the growing biotechnological trends for bioethanol production. Effective fermentation and assimilation of xylose, the major pentose sugar element of plant cell walls and the second most abundant carbohydrate, is a bottleneck step towards a robust biofuel production from agricultural waste materials. Hence, several attempts were implemented to engineer the conventional Saccharomyces cerevisiae yeast to transport and ferment xylose because naturally it does not use xylose, using genetic materials of Pichia stipitis, the pioneer native xylose fermenting yeast. Recently, the nonconventional yeast Spathaspora passalidarum appeared as a founder member of a new small group of yeasts that, like Pichia stipitis, can utilize and ferment xylose. Therefore, the understanding of the molecular mechanisms regulating the xylose assimilation in such pentose fermenting yeasts will enable us to eliminate the obstacles in the biofuels pipeline, and to develop industrial strains by means of genetic engineering to increase the availability of renewable biofuel products from agricultural biomass. In this review, we will highlight the recent advances in the field of native xylose metabolizing yeasts, with special emphasis on S. passalidarum for improving bioethanol production.

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Molecular cloning of alcohol dehydrogenase genes of the yeastPichia stipitis and identification of the fermentative ADH

Cited by 41 publications

References 54 publications

Adaptation of Dekkera bruxellensis to lignocellulose‐based substrate

Adaptation of Dekkera bruxellensis to lignocellulose‐based substrate

The Crucial Role of Alcohol Dehydrogenase Adh3 inKluyveromyces marxianusMitochondrial Metabolism

The Xylose Metabolizing Yeast Spathaspora passalidarum is a Promising Genetic Treasure for Improving Bioethanol Production

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