Molecular cloning of a novel human gene encoding histone acetyltransferase-like protein involved in transcriptional activation of hTERT

Liu, Jun-Jie; Liu, Haijing; Wang, Qiang; Tang, Zhengyang; Hou, Lin; Zhang, Bo

doi:10.1016/j.bbrc.2003.09.235

Cited by 64 publications

(51 citation statements)

References 18 publications

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“…S3A). These data indicated increased binding of cluster b proteins to heterochromatin in response to hypoxia stress, consistent with a role for these molecules in the repression of tumor suppressor genes during malignant progression (35,36). In agreement with our proteomic data, other investigators have recently reported a role for KDM5B in regulating the expression of E2f genes via remodeling of heterochromatin during cell cycle progression (37), and our Western blot analysis confirmed that HP1BP3 binding to chromatin was increased by hypoxia stress (supplemental Figs.…”

Section: Resultssupporting

confidence: 56%

Quantitative Profiling of Chromatome Dynamics Reveals a Novel Role for HP1BP3 in Hypoxia-induced Oncogenesis

Dutta

Ren

Lim

et al. 2014

Molecular & Cellular Proteomics

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In contrast to the intensely studied genetic and epigenetic changes that induce host cell transformation to initiate tumor development, those that promote the malignant progression of cancer remain poorly defined. As emerging evidence suggests that the hypoxic tumor microenvironment could re-model the chromatin-associated proteome (chromatome) to induce epigenetic changes and alter gene expression in cancer cells, we hypothesized that hypoxia-driven evolution of the chromatome promotes malignant changes and the development of therapy resistance in tumor cells. To test this hypothesis, we isolated chromatins from tumor cells treated with varying conditions of normoxia, hypoxia, and re-oxygenation and then partially digested them with DNase I and analyzed them for changes in euchromatin-and heterochromatinassociated proteins using an iTRAQ-based quantitative proteomic approach. We identified a total of 1446 proteins with a high level of confidence, including 819 proteins that were observed to change their chromatin association topology under hypoxic conditions. These hypoxia-sensitive proteins included key mediators of chromatin organization, transcriptional regulation, and DNA repair. Furthermore, our proteomic and functional experiments revealed a novel role for the chromatin organizer protein HP1BP3 in mediating chromatin condensation during hypoxia, leading to increased tumor cell viability, radio-resistance, chemo-resistance, and self-renewal. Taken together, our findings indicate that HP1BP3 is a key mediator of tumor progression and cancer cell acquisition of therapy-resistant traits, and thus might represent a novel therapeutic target in a range of human malignancies. Molecular &

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Section: Resultssupporting

confidence: 56%

Quantitative Profiling of Chromatome Dynamics Reveals a Novel Role for HP1BP3 in Hypoxia-induced Oncogenesis

Dutta

Ren

Lim

et al. 2014

Molecular & Cellular Proteomics

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Section: Discussionmentioning

confidence: 99%

“…Human NAT10 (also known as hALP) was initially identified as a transcriptional factor that interacts with the promoter region of hTERT (telomerase reverse transcriptase) (45). Recombinant NAT10/hALP (amino acids 164 -834) lacking the N-terminal domains had an ability to acetylate calf thymus histones in vitro in the presence of acetyl-CoA (without ATP), indicating that NAT10/hALP has a histone acetyltransferase activity that might promote hTERT transcription via decondensation of chromatin structure (45). However, based on the crystal structure of TmcA, the N-terminal domain of the recombinant NAT10/hALP that was missing from that construct contains a functionally important region (DUF1726) that constitutes the RNA helicase/ATPase domain required for the tRNA acetyltransferase activity (28,46).…”

Section: Discussionmentioning

confidence: 99%

A Single Acetylation of 18 S rRNA Is Essential for Biogenesis of the Small Ribosomal Subunit in Saccharomyces cerevisiae

Ito

Akamatsu

Noma

et al. 2014

Journal of Biological Chemistry

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Background: Post-transcriptional modifications of rRNAs play important roles in biogenesis and function of ribosome. Results: Identification of an essential RNA acetyltransferase Rra1p responsible for forming N 4 -acetylcytidine at position 1773 in 18 S rRNA. Conclusion: Rra1p and ac 4 C1773 are required for pre-18 S rRNA processing. Significance: Rra1p modulates 40 S subunit biogenesis through a single acetylation of 18 S rRNA by sensing nuclear acetyl-CoA concentration.

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“…To ask which HAT contributes this activity, we reasoned that such a HAT must be a nuclear membrane-associated moiety. An in silico search revealed that the human genome encodes a minimum of sixteen HATs (40, 41); however, only one, KIAA1709/hALP (41), is a nuclear membrane-associated protein (5).…”

Section: Determinants Of Hssun1 Localization To the Nuclearmentioning

confidence: 99%

Histone Acetyltransferase hALP and Nuclear Membrane Protein hsSUN1 Function in De-condensation of Mitotic Chromosomes

Chi

Haller

Péloponèse

et al. 2007

Journal of Biological Chemistry

100

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Replicated mammalian chromosomes condense to segregate during anaphase, and they de-condense at the conclusion of mitosis. Currently, it is not understood what the factors and events are that specify de-condensation. Here, we demonstrate that chromosome de-condensation needs the function of an inner nuclear membrane (INM) protein hsSUN1 and a membrane-associated histone acetyltransferase (HAT), hALP. We propose that nascently reforming nuclear envelope employs hsSUN1 and hALP to acetylate histones for de-compacting DNA at the end of mitosis.The eukaryotic nucleus is separated from other organelles by an envelope containing two membrane layers continuous with the endoplasmic reticulum. Nuclear membrane proteins fall into three categories according to their localization. The first group is the trans-nuclear membrane proteins resident in the nuclear pore complex (NPC).2 The second group contains the inner membrane proteins (INM), which include the lamin B receptor (LBR), emerin, and lamin-associated polypeptides (LAPs). The third group includes proteins underlying the nuclear membrane such as nuclear lamina (1). Functionally, the INM provides a physical barrier; the NPC serves for the transport of material between the nucleus and the cytoplasm (2); and the nuclear lamina erects a meshwork, which maintains nuclear structure and assists indirectly in DNA replication and RNA processing (3, 4).Most INM proteins are associated with the nuclear lamina. In a proteomic study of INM proteins, in addition to 13 known proteins, 67 uncharacterized open reading frames (ORFs) were identified (5

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Molecular cloning of a novel human gene encoding histone acetyltransferase-like protein involved in transcriptional activation of hTERT

Cited by 64 publications

References 18 publications

Quantitative Profiling of Chromatome Dynamics Reveals a Novel Role for HP1BP3 in Hypoxia-induced Oncogenesis

Quantitative Profiling of Chromatome Dynamics Reveals a Novel Role for HP1BP3 in Hypoxia-induced Oncogenesis

A Single Acetylation of 18 S rRNA Is Essential for Biogenesis of the Small Ribosomal Subunit in Saccharomyces cerevisiae

Histone Acetyltransferase hALP and Nuclear Membrane Protein hsSUN1 Function in De-condensation of Mitotic Chromosomes

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