Molecular cloning of a mammalian nuclear phosphoprotein NUCKS, which serves as a substrate for Cdk1 <i>in vivo</i>

Østvold, Anne Carine; Norum, Jens Henrik; Mathiesen, Siri B.W; Wanvik, Birgit; Sefland, Iren; Grundt, Kirsten

doi:10.1046/j.1432-1327.2001.02120.x

Cited by 66 publications

(50 citation statements)

References 45 publications

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“…NUCKS-1 from proliferating cells exhibits higher affinity for DNA when compared to NUCKS-1 from non-proliferating cells, NUCKS proteins are known and as the protein is highly expressed throughout the mammalian kingdom and serves as a substrate for Cdk1 in vivo (Ostvold et al, 2001).…”

Section: 837 Effect Of Nucks-1 Overexpression On Cytokines Profilingmentioning

confidence: 99%

Effect of NUCKS-1 Overexpression on Cytokine Profiling in Obese Women with Breast Cancer

Soliman

Zineldeen²,

El-Khadrawy³

2014

Asian Pacific Journal of Cancer Prevention

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Background: Overweight and obesity are recognized as major drivers of cancers including breast cancer. Several cytokines, including interleukin-6 (IL-6), IL-10 and lipocalin 2 (LCN2), as well as dysregulated cell cycle proteins are implicated in breast carcinogenesis. The nuclear, casein kinase and cyclin-dependent kinase substrate-1 (NUCKS-1), is a nuclear DNA-binding protein that has been implicated in several human cancers, including breast cancer. Objectives: The present study was conducted to evaluate NUCKS-1 mRNA expression in breast tissue from obese patients with and without breast cancer and lean controls. NUCKS-1 expression was correlated to cytokine profiles as prognostic and monitoring tools for breast cancer, providing a molecular basis for a causal link between obesity and risk. Materials and Methods: This study included 39 females with breast cancer (G III) that was furtherly subdivided into two subgroups according to cancer grading (G IIIa and G IIIb) and 10 control obese females (G II) in addition to 10 age-matched healthy lean controls (G I). NUCKS-1 expression was studied in breast tissue biopsies by means of real-time PCR (RT-PCR). Serum cytokine profiles were determined by immunoassay. Lipid profiles and glycemic status as well as anthropometric measures werealso recorded for all participants. Results: IL-6, IL-12 and LCN2 were significantly higher in control obese and breast cancer group than their relevant lean controls (p<0.05), while NUCKS-1 mRNA expression was significantly higher in the breast cancer group compared to the other groups (p<0.05). Significant higher levels of IL-6, IL-12, and LCN2 as well as NUCKS-1 mRNA levels were reported in G IIIb than G IIIa, and positively correlated with obesity markers in all obese patients. Conclusions: Evaluation of cytokine levels as well as related gene expression may provide a new tool for understanding interactions for three axes of carcinogenesis, innate immunity, inflammation and cell cycling, and hope for new strategies of management.

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Section: 837 Effect Of Nucks-1 Overexpression On Cytokines Profilingmentioning

confidence: 99%

Effect of NUCKS-1 Overexpression on Cytokine Profiling in Obese Women with Breast Cancer

Soliman

Zineldeen²,

El-Khadrawy³

2014

Asian Pacific Journal of Cancer Prevention

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“…NUCKS is similar (by sequence analysis) to the rat nuclear ubiquitous casein kinase 2, which was first isolated from both HeLa cells and rat brain and subsequently found to be ubiquitously expressed (Ostvold et al 2001). The protein localizes to the nucleus and binds single-and double-stranded DNA.…”

Section: ‫03מ‬mentioning

confidence: 92%

Identification and Confirmation of a Module of Coexpressed Genes

Thompson

Harris²,

Wold³

et al. 2002

Genome Res.

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We synthesize a large gene expression data set using dbEST and UniGene. We use guilt-by-association (GBA) to analyze this data set and identify coexpressed genes. One module, or group of genes, was found to be coexpressed mainly in tissue extracted from breast and ovarian cancers, but also found in tissue from lung cancers, brain cancers, and bone marrow. This module contains at least six members that are believed to be involved in either transcritional regulation (PDEF, H2AFO, NUCKS) or the ubiquitin proteasome pathway (PSMD7, SQSTM1, FLJ10111). We confirm these observations of coexpression by real-time RT–PCR analysis of mRNA extracted from four model breast epithelial cell lines

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“…Nuclear ubiquitous casein and cyclin-dependent kinases substrate (NUCKS) is a nuclear DNA binding protein occurring in almost all types of human cells [1,2]. Its biological functions are not well understood, but its structural similarity to the high mobility group A (HMGA) proteins suggests that it may play a role in regulation of chromatin structure and its activity [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

“…Until now NUCKS has been studied in detail using a variety of biochemical and cell biological methods [1,2,[7][8][9][10][11]. Some of these analyses related the occurrence of the protein to histological grade or to a particular tumor type and also to benign epithelial proliferations.…”

Section: Introductionmentioning

confidence: 99%

The comparison of nuclear ubiquitous casein and cyclin-dependent kinases substrate (NUCKS) with Ki67 proliferation marker expression in common skin tumors

Zduniak¹,

Agrawal²,

Symonowicz³

et al. 2014

pjp

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Nuclear ubiquitous casein and cyclin-dependent kinases substrate (NUCKS) is a chromosomal protein of unknown function. Its amino acid composition and structure of its DNA binding domain resemble those of high mobility group A (HMGA) proteins which are associated with various malignancies. Since changes in expression of HMGA are considered as a marker of tumor progression, it is possible that similar changes in expression of NUCKS could be a useful tool in diagnosis of malignant skin tumors. To investigate this assumption we used specific antibodies against NUCKS for immunohistochemistry of squamous (SCC) and basal cell carcinoma (BCC) as well as keratoacanthoma (KA). We found high expression of NU-CKS in nuclei of SCC and BCC cells which exceeded expression of the well-known proliferation marker Ki67. Expression of NUCKS in benign KA was much below that of malignant tumors. With the present study and based on our previous experience we would like to suggest the NUCKS protein as a novel proliferation marker for immunohistochemical evaluation of formalin-fixed and paraffin-embedded skin tumor specimens. We would like to emphasize that NUCKS abundance in malignant skin tumors is higher than that of the well-known proliferation marker Ki67, thus allowing more precise assessment of tumor proliferation potential.

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Molecular cloning of a mammalian nuclear phosphoprotein NUCKS, which serves as a substrate for Cdk1 in vivo

Cited by 66 publications

References 45 publications

Effect of NUCKS-1 Overexpression on Cytokine Profiling in Obese Women with Breast Cancer

Effect of NUCKS-1 Overexpression on Cytokine Profiling in Obese Women with Breast Cancer

Identification and Confirmation of a Module of Coexpressed Genes

The comparison of nuclear ubiquitous casein and cyclin-dependent kinases substrate (NUCKS) with Ki67 proliferation marker expression in common skin tumors

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