Molecular Cloning of a Human RP105 Homologue and Chromosomal Localization of the Mouse and Human RP105 Genes (Ly64andLY64)

Miura, Yoshiaki; Miyake, Kensuke; Yamashita, Yoshio; Shimazu, Rintaro; Copeland, Neal G.; Gilbert, Debra J.; Jenkins, Nancy A.; Inazawa, Johji; Abe, Tatsuo; Kimoto, Masao

doi:10.1006/geno.1996.0632

Cited by 43 publications

(43 citation statements)

References 3 publications

(3 reference statements)

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“…HGS 486245 diverges from the reported human cDNA sequences at nucleotide 2084 where the insertion of an additional thymidine results in a stop codon and premature truncation of the protein, resulting in the loss of the 11-amino-acid cytoplasmic domain. Furthermore, sequence analysis of additional RP105 clones isolated from human adult spleen, tonsil, and B cell lymphoma cDNA libraries, in-house, demonstrated that they were identical to those recently reported from spleen and Daudi B cells [8,9] lacking the thymidine insertion. The functional significance of the carboxy-terminal deletion found in the fetal library clone is not known.…”

Section: Discussionsupporting

confidence: 66%

“…Miura et al demonstrated RP105 mRNA levels in the human mature B cell lines, Ramos and Daudi, but not in the pre B cell line Nalm-6 or T cell lines CEM and Molt 4 [8]. Distinct from this, we identified RP105 mRNA in B cell lines Mono 6, Reh, Bjab, and Daudi but not in the Jurkat T cell leukemia line.…”

Section: Discussioncontrasting

confidence: 45%

“…The clone containing HGS 486245 was sequenced bidirectionally and determined to contain a 2775-bp insert, which represented an apparent full-length human RP105 -2092). Additional RP105 clones were identified in library sources including adult human spleen, tonsil, and B cell lymphoma and 3Ј sequence analysis demonstrated that these clones were identical to those recently reported [8,9].…”

Section: Resultsmentioning

confidence: 67%

“…Just recently two other groups reported the cloning of human homologs that are identical to our RP105 clone [8,9]. Consistent with the murine RP105, these laboratories describe the presence of transcript in several B cell lines and in B lymphocytes isolated from human tonsil, indicating that the human gene may also be expressed predominantly by B lymphocytes [8]. It is interesting to note that in the report by Fugier-Vivier et al the RP105 transcript was shown to also exist in tonsillar dendritic cells, which may suggest a role in germinal center function [9].…”

Section: Introductionmentioning

confidence: 86%

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Anti-human RP105 sera induces lymphocyte proliferation

Roshak

Anderson

Holmes

et al. 1999

Journal of Leukocyte Biology

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Cellular environment dictates whether antigen binding to the B lymphocyte receptor together with co-stimulatory molecules will result in proliferation, anergy, or apoptosis. Murine RP105 is a member of the leucine-rich repeat family of proteins, which is specifically expressed on mature B cells. Monoclonal antibodies to the murine RP105 induce proliferation and protect B cells from apoptosis, suggesting an important regulatory role in murine B lymphocyte function. We identified a human RP105 homolog and mapped the gene to chromosome 5q12.3-13.1. Tissue distribution analysis shows that the transcript is found predominately in lymphoid tissues including spleen, tonsils, appendix, and peripheral blood leukocytes. Polymerase chain reaction analysis of isolated primary human cell populations confirms that mRNA exists in spleen B lymphocytes and monocytes but not T lymphocytes. Western blot analysis demonstrates specific expression of human RP105 in human B lymphocytes. Murine anti-human RP105 sera was generated using DNA immunization. The antisera contained antibodies that recognized and bound to human B lymphocytes from both spleen and peripheral blood as assessed by flow cytometry. Assessment of biological function showed that human peripheral blood leukocytes incubated with anti-RP105 sera were induced to proliferate as measured by tritiated thymidine incorporation. Moreover, anti-CD40 and interleukin-4-treated cells but not those exposed to anti-RP105 sera produced soluble CD23, suggesting distinct functional roles. This is the first demonstration of both the existence of RP105 protein on human B lymphocytes and its role in the regulation of B lymphocyte activation.J. Leukoc. Biol. 65: 43-49; 1999.

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Section: Discussionsupporting

confidence: 66%

Section: Discussioncontrasting

confidence: 45%

Section: Resultsmentioning

confidence: 67%

Section: Introductionmentioning

confidence: 86%

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Anti-human RP105 sera induces lymphocyte proliferation

Roshak

Anderson

Holmes

et al. 1999

Journal of Leukocyte Biology

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“…Similarly, CD180 belongs to a family of pathogen receptors known as Toll-like receptors (TLRs). CD180/MD-1 works in concert with TLR4 to control the recognition of B cells and signaling of lipopolysaccharide, which is a constituent of the cellular membrane of gram-negative bacteria (Miura et al, 1996). Our results indicated that the CD1 gene family, including CD1B and CD1D, was upregulated in line M5.1 whereas CD1D was downregulated in line M15.2 (Fig.…”

Section: Avian β-Defensin Genes Are Differentially Expressed To Ne-inmentioning

confidence: 64%

Analysis of Differentially Expressed Genes in Necrotic Enteritis-infected Fayoumi Chickens using RNA Sequencing

et al. 2017

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We identified and evaluated differentially expressed genes (DEGs) by RNA-Sequencing (RNA-Seq) in the intestinal mucosa of two Fayoumi chicken lines, M5.1 and M15.2, that are affected by necrotic enteritis (NE); these chicken lines share the same genetic background but have different major histocompatibility complexes. RNA-Seq generated over 49 and 40 million reads for lines M5.1 and M15.2, respectively. The alignment of these sequences with the Gallus gallus genome database revealed the expression of more than 14,500 genes in two lines, among which 581, 1270, and 1140 DEGs were detected when lines M15.2 and M5.1 were compared with the control and compared between each other. The analysis of all DEGs using the gene ontology database revealed annotations for 111 biological processes, 32 cellular components, and 17 molecular functions, and KEEG pathway mapping indicated that the DEGs were primarily involved in immunity, responses to various stimuli, and signal transduction. In addition, we analyzed 183 innate immune genes that were differentially expressed in NE-induced chicken lines, including 46 CD molecular genes, 89 immune-related genes, and 13 β-defensin genes with 3 lineage-specific duplications. Taken together, the transcriptional profiles showed that line M5.1 was more resistant to NE than line M15.2 and that differential gene expression patterns were associated with host genetic differences in resistance to NE. qRT-PCR and RNA-Seq analyses showed that all the genes examined had similar responses to NE (correlation coefficient R＝0.84 to 0.88, p＜0.01) in both lines. To the best of our knowledge, this is the first study that describes NE-induced DEGs using RNA-seq in two lines with different levels of susceptibility to NE. These results will lead to increased insights on NE disease resistance mechanisms and the role of host genes in the control of the host immune response.

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