Molecular cloning of a gene involved in glucose sensing in the yeast Saccharomyces cerevisiae

Abstract

Cells of the yeast Saccharomyces cerevisiae display a wide range of glucose-induced regulatory phenomena, including glucose-induced activation of the RAS-adenylate cyclase pathway and phosphatidylinositol turnover, rapid post-translational effects on the activity of different enzymes as well as long-term effects at the transcriptional level. A gene called GGS1 (for General Glucose Sensor) that is apparently required for the glucose-induced regulatory effects and several ggs1 alleles (fdp1, byp1 and cif1) has been cloned and characterized. A GGS1 homologue is present in Methanobacterium thermoautotrophicum. Yeast ggs1 mutants are unable to grow on glucose or related readily fermentable sugars, apparently owing to unrestricted influx of sugar into glycolysis, resulting in its rapid deregulation. Levels of intracellular free glucose and metabolites measured over a period of a few minutes after addition of glucose to cells of a ggs1 delta strain are consistent with our previous suggestion of a functional interaction between a sugar transporter, a sugar kinase and the GGS1 gene product. Such a glucose-sensing system might both restrict the influx of glucose and activate several signal transduction pathways, leading to the wide range of glucose-induced regulatory phenomena. Deregulation of these pathways in ggs1 mutants might explain phenotypic defects observed in the absence of glucose, e.g. the inability of ggs1 diploids to sporulate.

Structural analysis of the subunits of the trehalose‐6‐phosphate synthase/phosphatase complex in Saccharomyces cerevisiae and their function during heat shock

“…The strategies for deletion of TPS1 (Van Aelst et al, 1993) and TPS2 have been described elsewhere. For deletion of TSL1, a plasmid carrying the TSL1 open reading frame as well as 5Ј and 3Ј flanking sequences cloned into the SmaI site of pBluescript (Stratagene) was amplified by the polymerase chain reaction (PCR), using primers that yield a PCR product lacking the complete open reading frame.…”

A bifunctional TPS–TPP enzyme from yeast confers tolerance to multiple and extreme abiotic-stress conditions in transgenic Arabidopsis

“…1b). These mutants are unable to grow with glucose as the carbon source due to the role of TPS in regulating the Xow of glucose into glycolysis (Van Aelst et al 1993;Thevelein and Hohmann 1995). Also, the tps2 and tps1 tps2 mutants transformed with TPS1-TPS2 gene were able to grow at 38.5°C, restoring yeast thermotolerance (Fig.…”

“…Additionally, the yeast tps1 mutant and its alleles are unable to grow in glucose as the sole carbon source. It has been suggested that this defect may be due to the additional role of the TPS subunit in regulating the Xow of glucose into glycolysis (Van Aelst et al 1993;Thevelein and Hohmann 1995).…”

The role of trehalose synthesis for the acquisition of thermotolerance in yeast

“…It is now well established that the mutations bypl, cijl, fdpl, ggsl, glc6, tpsl and tssl are all alleles of the same gene Cannon et al, 1992;Vuorio et al, 1992;Stucka and Blizquez, 1993;Van Aelst et al, 1993). For the sake of lucidity, we use the common name TPSl for the designation of this gene.…”