Molecular cloning of a cDNA encoding vitellogenesis-inhibiting hormone in the whiteleg shrimp Litopenaeus vannamei and preparation of its recombinant peptide using an E. coli expression system

Tsutsui, Naoaki; Ohira, Tsuyoshi; Okutsu, Tomoyuki; Shinji, Junpei; Bae, Sun Hye; Kang, Bong Jung; Wilder, Marcy N.

doi:10.1007/s12562-013-0603-z

Cited by 15 publications

(17 citation statements)

References 27 publications

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“…Recombinant VIH of L. vannamei (Liv-rVIH) was obtained as previously reported for LivrSGP-G [17]. Briefly, DNA for Liv-SGP-G was amplified by PCR and the first Ala residue in natural Liv-SGP-G was substituted with a Gly residue (Liv-SGP-G-Gly; the precursor form of Liv-SGP-G).…”

Section: Recombinant Vih and Polyclonal Antibodymentioning

confidence: 99%

Dynamics of Vitellogenin and Vitellogenesis-Inhibiting Hormone Levels in Adult and Subadult Whiteleg Shrimp, Litopenaeus vannamei: Relation to Molting and Eyestalk Ablation1

Kang

Okutsu

Tsutsui

et al. 2014

Biology of Reproduction

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Levels of vitellogenin (VG) and vitellogenesis-inhibiting hormone (VIH) in the whiteleg shrimp, Litopenaeus vannamei, were measured by time-resolved fluoroimmunoassay in relation to the molting cycle and ovarian maturation induced by eyestalk ablation. During the molt cycle, VG mRNA expression levels and VG concentrations showed similar patterns of fluctuation. VG levels increased significantly at early intermolt (stage C0) in adults, but not in subadults. Unilateral and bilateral eyestalk ablation increased VG levels in adults, whereas only bilateral eyestalk ablation affected subadults. VIH levels showed contrasting patterns between adults and subadults. In adults, levels were high in late postmolt adults (stage B) and then low thereafter, whereas they increased from postmolt (stage A) to intermolt (stage C0) in subadults and remained high. Unilateral eyestalk ablation increased VIH levels 10 days following ablation in adults, after which levels decreased at 20 days. VIH levels decreased from 10 to 20 days after bilateral ablation. Both unilateral and bilateral ablation led to increased VIH levels in subadults. Eyestalk ablation induced ovarian maturation, but did not reduce VIH concentrations in the hemolymph. This phenomenon was perhaps due to other crustacean hyperglycemic hormone peptides having cross-reactivity with VIH antibodies. This is the first report to quantify concentrations of VG and VIH together in L. vannamei hemolymph, and to examine their relative dynamics.

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Section: Recombinant Vih and Polyclonal Antibodymentioning

confidence: 99%

Dynamics of Vitellogenin and Vitellogenesis-Inhibiting Hormone Levels in Adult and Subadult Whiteleg Shrimp, Litopenaeus vannamei: Relation to Molting and Eyestalk Ablation1

Kang

Okutsu

Tsutsui

et al. 2014

Biology of Reproduction

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“…Double-stranded RNA (dsRNA) targeting the GIH gene was able to induce ovarian maturation in female P. monodon (Treerattrakool et al 2008), and a single injection of an anti-GIH mAb into wild previtellogenic P. monodon was able to induce ovarian maturation and spawning at a comparable rate to the eyestalk ablation (Treerattrakool et al 2014). In addition, recombinant GIHs expressed in bacterial cells were able to reduce Vtg mRNA to the same level as that of the natural Liv-SGP-G (previously deemed another candidate of LvVIH) (Tsutsui et al 2013) in Marsupenaeus japonicus ) and L. vannamei (Chen et al 2014), suggesting that GIH plays a prominent role in the regulation of reproduction.…”

Section: Introductionmentioning

confidence: 81%

“…This difference could be due to individual polymorphisms within the genes. However, Tsutsui et al (2013) found that SGP-G (another potential LvVIH protein) precursor identified in L. vannamei possessed the structure of a typical type I peptide. Whether LvGIH and LvSGP-G are two differently spliced variants is not clear.…”

Section: Discussionmentioning

confidence: 99%

“…Members of the CHH family contain six conserved cysteine residues and can be divided into two subfamilies, Type I (CHH/ITP) and Type II (MIH, MOIH, GIH/VIH), according to their amino acid sequences and precursor structures (Chan et al 2003). GIH (also known as vitellogenesis inhibiting hormone, VIH) is a member of the CHH family (Edomi et al 2002;Lacombe et al 1999) and since the first report of the primary structure of two VIH isoforms from the lobster, Homarus americanus (Soyez et al 1991), many putative GIH or VIH sequences have been reported from a variety of species including P. monodon (Treerattrakool et al 2008), Nephrops norvegicus (Edomi et al 2002), L. vannamei (Tsutsui et al 2013;Chen et al 2014), Procambarus bouvieri (Aguilar et al 1992), and H. americanus (De Kleijn et al 1994). The majority of the GIH peptides comprise 77-83 amino acids with a molecular mass between 7 and 11 kDa, and they share a high degree of amino acid sequence similarity.…”

Section: Introductionmentioning

confidence: 99%

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cDNA cloning, expression, and immunolocalization of gonad-inhibiting hormone (GIH) in Litopenaeus vannamei

Deng

Jiang

et al. 2015

Genes Genom

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In this study, the full-length GIH cDNA sequence from Litopenaeus vannamei was cloned from the eyestalk by reverse transcriptase polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends. The fulllength GIH cDNA was 865 bp with a 288 bp open-reading frame, which encoded a 96 amino acid prepro-GIH with 17 amino acid signal peptide. L. vannamei GIH (LvGIH) can be classified as a member of type-II crustacean hyperglycemic hormone polypeptide family. LvGIH shares 93.8 and 66.7 % amino acid sequence identity with GIH from Penaeus monodon, and the molt-inhibiting hormone from Marsupenaeus japonicas, respectively. By quantitative real-time PCR (qPCR), LvGIH mRNA transcripts were detected in fertilized eggs, nauplius, zoea, mysis and juveniles of 25, 35 and 40 days old. LvGIH transcript levels increased significantly with the development from fertilized eggs to juveniles. LvGIH transcript levels were highest in juveniles at 35 days old. By RT-PCR, LvGIH mRNA transcripts were detected only in the eyestalks and brains but not in the muscles, intestines, gills, heart, hepatopancreas, ovaries and testes of adults, and there was no difference in the expression level of LvGIH between males and females. Using the P. monodon anti-GIH antibody, we showed that LvGIH was located mainly in the XO-SG and slightly in axon, with similar fluorescence intensity found in XO and SG. To summarize, we have cloned and characterized the GIH of the shrimp L. vannamei. In addition to the GIH properties described in other crustaceans, a peak of LvGIH expression was identified at the time of sexual differentiation (i.e., day 35 larvae) suggesting that LvGIH may also be involved in the control of this process.

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“…In different crustacean species, VTG is synthesized only in the ovary [8,17], or only in the hepatopancreas [10,27], or both in the ovary and hepatopancreas [7,20], and then transported to the oocytes subsequent accumulated in the ooplasm as vitellin after further modification. The process of vitellogenesis in crustaceans is widely assumed to be negatively controlled by VIH [4,23,26]. However, recent studies revealed that other members of CHH family may also play a role in regulating vitellogenesis [5,12,25,29].…”

Section: Introductionmentioning

confidence: 99%

Differential regulation of hepatopancreatic vitellogenin (VTG) gene expression by two putative molt-inhibiting hormones (MIH1/2) in Pacific white shrimp (Litopenaeus vannamei)

et al. 2015

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Molecular cloning of a cDNA encoding vitellogenesis-inhibiting hormone in the whiteleg shrimp Litopenaeus vannamei and preparation of its recombinant peptide using an E. coli expression system

Cited by 15 publications

References 27 publications

Dynamics of Vitellogenin and Vitellogenesis-Inhibiting Hormone Levels in Adult and Subadult Whiteleg Shrimp, Litopenaeus vannamei: Relation to Molting and Eyestalk Ablation1

Dynamics of Vitellogenin and Vitellogenesis-Inhibiting Hormone Levels in Adult and Subadult Whiteleg Shrimp, Litopenaeus vannamei: Relation to Molting and Eyestalk Ablation1

cDNA cloning, expression, and immunolocalization of gonad-inhibiting hormone (GIH) in Litopenaeus vannamei

Differential regulation of hepatopancreatic vitellogenin (VTG) gene expression by two putative molt-inhibiting hormones (MIH1/2) in Pacific white shrimp (Litopenaeus vannamei)

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