Molecular cloning of a C-type lectin (LvLT) from the shrimp Litopenaeus vannamei: Early gene down-regulation after WSSV infection

Tung, Tracy Hoi; Tiu, Shirley Hiu Kwan; He, Jianguo; Chan, Siu-Ming

doi:10.1016/j.fsi.2006.12.005

Cited by 124 publications

(76 citation statements)

References 19 publications

(19 reference statements)

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“…A growing number of CTLs have recently been discovered from marine invertebrates, especially crustaceans and molluscs, including those from F. chinensis [16,21,46,47], L. vannamei [15,19,45,48], Penaeus monodon [49,50], C. farreri [18,23,24,26], A. irradians [22,25] and Crassostrea gigas [51], Crassostrea virginica [52], and Haliotis discus discus [17]. Relatively little information is available on CTLs in echinoderms, and a handful of sea cucumber CTLs have been reported, such as MBL-C (Cucumaria japonica) [53], SJL-I (S. japonicus) [35,36], MBL-AJ and AJCTL (A. japonicus) [37,38].…”

Section: Discussionmentioning

confidence: 99%

Critical roles of sea cucumber C-type lectin in non-self recognition and bacterial clearance

Wei

Liu

Yang

et al. 2015

Fish & Shellfish Immunology

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a b s t r a c t C-type lectin is one important pattern recognition receptor (PRR) that plays crucial roles in multiple immune responses. A C-type lectin from sea cucumber Apostichopus japonicus (AjCTL-1) was characterized in the present study. The amino acid sequence of AjCTL-1 shared high similarities with other Ctype lectins from invertebrates and vertebrates. The C-type lectin domain (CTLD) of AjCTL-1 contained a Ca 2þ -binding site 2 and four conserved cysteine residues. AjCTL-1 mRNA expression patterns in tissues and after bacterial challenge were then analysed. Quantitative PCR revealed that AjCTL-1 mRNA was widely expressed in the tested tissues of healthy sea cucumber. The highest expression level occurred in gonad followed by body wall, coelomocytes, tentacle, intestinum and longitudinal muscle, and the lowest expression level was in respiratory tree. AjCTL-1 mRNA expression in coelomocytes was significantly induced by gram-negative Listonella anguillarum and gram-positive Micrococcus luteus, with different upregulation patterns post-challenge. Recombinant AjCTL-1 exhibited the ability to bind peptidoglycan directly, agglutinate M. luteus, Staphylococcus aureus and Escherichia coli, in a Ca 2þ -dependant manner, and enhance the phagocytosis of coelomocytes against E. coli in vitro. The results indicated that AjCTL-1 could act as a PRR in Apostichopus japonicus and had critical roles in non-self recognition and bacterial clearance against invading microbes.

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Section: Discussionmentioning

confidence: 99%

Critical roles of sea cucumber C-type lectin in non-self recognition and bacterial clearance

Wei

Liu

Yang

et al. 2015

Fish & Shellfish Immunology

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“…Previous studies have demonstrated that C-type lectins play a key role in immune recognition in invertebrates as PRRs [14,25,26]. However, the research of C-type lectins in marine mollusks is still in the early stage.…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning and expression of a C-type lectin gene from Venerupis philippinarum

Chen

Zhao

2013

Mol Biol Rep

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C-type lectins have been demonstrated to play important roles in invertebrate innate immunity by mediating the recognition of pathogens and clearing the microinvaders. In the present study, a C-type lectin gene (denoted as VpCTL) was identified from Venerupis philippinarum by expressed sequence tag and rapid amplification of cDNA ends approaches. The full-length cDNA of VpCTL consists of 904 nucleotides with an open-reading frame of 456 bp encoding a peptide of 151 amino acids. The deduced amino acid sequence of VpCTL shared high similarity with C-type lectins from other species. The C-type lectin domain and the characteristic EPN and WND motifs were found in VpCTL. The VpCTL mRNA was dominantly expressed in the haemocytes of the V. philippinarum. After Listonella anguillarum challenge, the temporal expression of VpCTL mRNA in haemocytes was increased by 97-and 84-fold at 48 and 96 h, respectively. With high expression level in haemocytes and hepatopancreas, and the up-regulated expression in haemocytes indicted that VpCTL was perhaps involved in the immune responses to L. anguillarum challenge.

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“…While three Ctype lectins from shrimp M. japonicus could interact with several envelope proteins of WSSV and recognize a wide range of microorganisms [17]. Up to now, four C-type lectins of the shrimp L. vannamei were reported, two of them were proved to agglutinate Gram-negative bacteria [18,23], and the other two might be related to the WSSV infection [24,27]. Discovering new C-type lectins from crustaceans and investigating their immune functions were helpful to understand the diversity of the invertebrate C-type lectins.…”

Section: Discussionmentioning

confidence: 99%

“…Since its outbreak in 1992, WSSV had caused great economic loss in the world [20]. Recently, researches confirmed that several C-type lectins, such as FcLec3 [20], MjLecA, MjLecB, MjLecC [17], LvLT [24] and LvCTL1 [27], played an important role in the process of shrimp defense against WSSV infection. In this study, WSSV infection significantly increased the expression level of LvLectin-2 at 6 and 12 h, indicating that the multi-recognition characteristics of LvLectin-2 may be important in the early stage of WSSV infection.…”

Section: Discussionmentioning

confidence: 99%

Two C-type lectins from shrimp Litopenaeus vannamei that might be involved in immune response against bacteria and virus

Wei

Liu

Yang

et al. 2012

Fish & Shellfish Immunology

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Molecular cloning of a C-type lectin (LvLT) from the shrimp Litopenaeus vannamei: Early gene down-regulation after WSSV infection

Cited by 124 publications

References 19 publications

Critical roles of sea cucumber C-type lectin in non-self recognition and bacterial clearance

Critical roles of sea cucumber C-type lectin in non-self recognition and bacterial clearance

Molecular cloning and expression of a C-type lectin gene from Venerupis philippinarum

Two C-type lectins from shrimp Litopenaeus vannamei that might be involved in immune response against bacteria and virus

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