Molecular Cloning, Gene Localization, and Structure of Human Cyclin B3

Lozano, Jean-Claude; Perret, Eric; Schatt, Philippe; Arnould, Cécile; Peaucellier, Gérard; Picard, André

doi:10.1006/bbrc.2002.6458

Cited by 35 publications

(48 citation statements)

References 11 publications

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“…6C and data not shown). This result is in marked contrast to that of Lozano and colleagues (25) who reported mRNA in other tissues after two rounds of nested RT-PCR. However, our single round PCR protocol was sufficient to detect a single copy of cDNA per cell, and we were unable to detect cyclin B3 in thymus.…”

Section: Identification Of Human Cyclin B3-contrasting

confidence: 99%

“…6B). While this manuscript was in preparation, similar Northern results were reported for different N-and C-terminal probes (25). These signals were only apparent as a single 5 kb band, suggesting that the B3 probe sequences might be contiguous.…”

Section: Identification Of Human Cyclin B3-supporting

confidence: 76%

“…This mouse cDNA and genomic sequences mapped to a region of the X chromosome XA2-A3 (data not shown) which was syntenic with the region Xp11. 22-11.23 (28), where human cyclin B3 was mapped (25).…”

Section: Mouse Cyclin B3 Mrna Is Expressed In Leptotene and Zygotene mentioning

confidence: 99%

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Characterization and Expression of Mammalian Cyclin B3, a Prepachytene Meiotic Cyclin

Nguyen¹,

Manova²,

Capodieci³

et al. 2002

Journal of Biological Chemistry

134

135

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We report the identification and expression pattern of a full-length human cDNA and a partial mouse cDNA encoding cyclin B3. Cyclin B3 (CCNB3) is conserved from Caenorhabditis elegans to Homo sapiens and has an undefined meiotic function in female, but not male Drosophila melanogaster. We show that H. sapiens cyclin B3 interacts with cdk2, is localized to the nucleus, and is degraded during anaphase entry after the degradation of cyclin B1. Degradation is dependent on sequences conserved in a destruction box motif. Overexpression of nondegradable cyclin B3 blocks the mitotic cell cycle in late anaphase, and at higher doses it can interfere with progression through G 1 and entry into S phase. H. sapiens cyclin B3 mRNA and protein are detected readily in developing germ cells in the human testis and not in any other tissue. The mouse cDNA has allowed us to further localize cyclin B3 mRNA to leptotene and zygotene spermatocytes. The expression pattern of mammalian cyclin B3 suggests that it may be important for events occurring in early meiotic prophase I.Cyclins are positive regulatory subunits of the cyclin-dependent kinases (cdks).1 Cyclins increase kinase activity by inducing structural changes in the cdk (1). Cyclins also provide substrate binding motifs (2, 3), signals that localize cdks (4, 5), and motifs that allow the incorporation of the cdk into higher order molecular complexes (6, 7). Although first identified as regulators of mitotic cell cycle transitions, cyclin-cdk complexes have other roles: cyclin C-cdk8 and cyclin H-cdk7 regulate transcription as part of the polymerase II holoenzyme (8 -10), pho80-pho85 regulates the localization of the pho4 transcription factor (11), p35-cdk5 regulates neuronal function (12, 13), and cyclin A1, with an unidentified cdk, regulates the pachytene to diplotene transition during male gametogenesis (14).Originally cloned from a chicken cDNA library, cyclin B3 is expressed at very low levels in virally transformed hematopoietic cell lines and early embryonic stages of chicken. When Gallus gallus cyclin B3 is expressed in HeLa cells, it is nuclear, interacts with cdk2 and cdc2, and is associated with a modest histone H1 kinase activity (15). In Drosophila, cyclin B3 is expressed in both mitotic and meiotic cells. In mitotic cells, it is only required when either cyclin A or cyclin B1 is absent. Its abundance is down-regulated during the metaphase to anaphase transition, following degradation of cyclin B1 (16). Overexpression of a nondegradable cyclin B3 is associated with chromatin decondensation defects at the end of mitosis (17). Cyclin B3 is essential for fertility; but unlike cyclin B1 deficiency, infertility of cyclin B3-deficient flies is restricted to the female germ line and does not appear to involve defects in ovary structure or in meiosis I entry (16).We report the cloning of a full-length human cyclin B3 cDNA and a partial mouse cyclin B3 cDNA. Like the previously described chicken and Drosophila homologs, when mammalian cyclin B3 expression is enforced in m...

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Section: Identification Of Human Cyclin B3-contrasting

confidence: 99%

Section: Identification Of Human Cyclin B3-supporting

confidence: 76%

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Characterization and Expression of Mammalian Cyclin B3, a Prepachytene Meiotic Cyclin

Nguyen¹,

Manova²,

Capodieci³

et al. 2002

Journal of Biological Chemistry

134

135

View full text Add to dashboard Cite

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“…Three mammalian B-type cyclins have been identified, B1, B2 and B3 (Pines and Hunter, 1989;Chapman and Wolgemuth, 1992;Gallant and Nigg, 1992;Lozano et al, 2002;Nguyen et al, 2002). Cyclins B1 and B2 are expressed in the majority of proliferating cells and localize to different compartments of the cell.…”

Section: Cyclins and Cdks In Mammalian Cell-cycle Regulationmentioning

confidence: 99%

Mammalian cell-cycle regulation: several Cdks, numerous cyclins and diverse compensatory mechanisms

2009

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After a decade of extensive work on gene knockout mouse models of cell-cycle regulators, the classical model of cell-cycle regulation was seriously challenged. Several unexpected compensatory mechanisms were uncovered among cyclins and Cdks in these studies. The most astonishing observation is that Cdk2 is dispensable for the regulation of the mitotic cell cycle with both Cdk4 and Cdk1 covering for Cdk2's functions. Similar to yeast, it was recently discovered that Cdk1 alone can drive the mammalian cell cycle, indicating that the regulation of the mammalian cell cycle is highly conserved. Nevertheless, cell-cycle-independent functions of Cdks and cyclins such as in DNA damage repair are still under investigation. Here we review the compensatory mechanisms among major cyclins and Cdks in mammalian cell-cycle regulation.

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“…Three mammalian B-type cyclins has been described: B1 (Pines and Hunter, 1989;Chapman and Wolgemuth, 1992), B2 (Chapman and Wolgemuth, 1993) and B3 (Gallant and Nigg, 1994;Lozano et al, 2002;Nguyen et al, 2002). Cyclins B1 and B2 associate with CDK1 (Draetta et al, 1989;Pines and Hunter, 1989), while mammalian cyclin B3 was shown to interact with CDK2 but not with CDK1 (Nguyen et al, 2002).…”

Section: Major M-phase Regulatorsmentioning

confidence: 99%

Cell cycle in mouse development

2005

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Mice likely represent the most-studied mammalian organism, except for humans. Genetic engineering in embryonic stem cells has allowed derivation of mouse strains lacking particular cell cycle proteins. Analyses of these mutant mice, and cells derived from them, facilitated the studies of the functions of cell cycle apparatus at the organismal and cellular levels. In this review, we give some background about the cell cycle progression during mouse development. We next discuss some insights about in vivo functions of the cell cycle proteins, gleaned from mouse knockout experiments. Our text is meant to provide examples of the recent experiments, rather than to supply an extensive and complete list.

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Molecular Cloning, Gene Localization, and Structure of Human Cyclin B3

Cited by 35 publications

References 11 publications

Characterization and Expression of Mammalian Cyclin B3, a Prepachytene Meiotic Cyclin

Characterization and Expression of Mammalian Cyclin B3, a Prepachytene Meiotic Cyclin

Mammalian cell-cycle regulation: several Cdks, numerous cyclins and diverse compensatory mechanisms

Cell cycle in mouse development

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