Molecular cloning, functional analysis and localization of a novel gene encoding polygalacturonase-inhibiting protein in Chorispora bungeana

Abstract

Polygalacturonase-inhibiting proteins (PGIPs) are plant defense proteins. To date, no spatial distribution of PGIPs and interaction between PGIPs and nitric oxide (NO) in plant were described. Here, we first reported the full-length cDNA sequence of PGIP of Chorispora bungeana (CbPGIP1). Notably, immunofluorescence localization showed that the CbPGIP was evenly distributed in leaves but it was mainly localized in epidermis and vascular bundle in stems and roots. Further studies indicated that CbPGIP had higher abundance in roots than in stems and leaves. Conversely, the bulk PGIP of C. bungeana showed a higher activity in leaves than in stems and roots. In addition, quantitative real-time polymerase chain reaction demonstrated that CbPGIP1 expression was induced by Stemphylium solani, salicylic acid (SA), 4, -4 degrees C and NO. This is a first report attempting to predict if NO can induce the PGIP expression. Taken together, these findings showed that the gene was spatially regulated and NO and SA might take part in CbPGIP1 expression induced by biotic and abiotic stresses. This study highlighted the potential importance of CbPGIP1 and NO in plant resistance.

Chilling- and Freezing- Induced Alterations in Cytosine Methylation and Its Association with the Cold Tolerance of an Alpine Subnival Plant, Chorispora bungeana

“…Transcriptome profiling and molecular genetics have analyzed the stress regulatory network of C . bungeana , a subnival alpine plant with inherited cold tolerance [ 33 – 37 , 39 , 40 ], but little is known about the direct effects of low temperature on epigenetic regulation. Using a combination of the MS-AFLP technique and integrated statistical analyses, we found an interesting variation in DNA methylation of C .…”

“…The gene-specific primers for real-time PCR analysis were designed using the www.idtdna.com/Primerquest and Primer Premier (version 5.0) software (PREMIER Biosoft). The primer pairs that were used were CBT10872 ( and ), CBT28565 ( and ), CBT12464 ( and ), CbADH1 (CBT4440) ( and ), CbUGT (CBT18111) ( and ), and CbPGIP (EU441203) [ 33 ] ( and ).…”

“…& C.A. Mey is a perennial alpine subnival plant species that naturally grows and survives in the alpine regions (2000–4200 m) in Asia [ 33 , 34 ]. As previous studies have reported, C .…”

Chitosan and ß-amino butyric acid up-regulates transcripts of resistance gene analog RGPM213 in pearl millet to infection by downy mildew pathogen

“…In Chorispora bungeana, immunolocalization of defense-related proteins in leaves, stems and roots have shown copious fluorescence in leaves, mainly localized in epidermis, and recorded high in vascular bundles of stems and roots. The fluorescence signal of the control was nearly absent in stem, but not in leaf and root [37]. In Phaseolus vulgaris, during compatible interactions, no significant accumulation of defense-related protein was observed in hypocotyls and a very weak accumulation seen in leaves during lesion formation in Phaseolus vulgaris [38].…”

Spatial distribution of polygalacturonase-inhibiting proteins in Arabidopsis and their expression induced by Stemphylium solani infection

“…In our previous study, we reported CbPGIP1 was induced by S. solani infection (Di et al, 2009). In order to test individual AtPGIP gene expression mediated by S. solani infection, we performed qRT-PCR to assay AtPGIP1 and AtPGIP2 expression in Arabidopsis under disease stress.…”