Molecular cloning, expression profiles and subcellular localization of cyclin B in ovary of the mud crab, Scylla paramamosain

Li, Wen‐Xing; Huang, Huiyang; Huang, Jingru; Yu, Jinjin; Ma, Jun; Ye, Haihui

doi:10.1007/s13258-013-0077-5

Cited by 11 publications

(13 citation statements)

References 47 publications

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“…This expression pattern is consistent with that previously described for cyclin B protein in mud crabs (Li et al 2013). Up-regulation of Pmcyclin B at stage II is consistent with the proposed role of cyclin B in mitosis proliferation of oogonia (Li et al 2013). Oocytes undergo active yolk accumulation at ovarian maturation stages III and IV (the developing and almost-ripe stages) and mature oocytes resume meiosis and, finally, arrest at the metaphase of meiosis I at stages V and VI (the ripe and spent stages) (Shangguan and Hunt, 1992;Huang et al, 2005;Li et al, 2013).…”

Section: Discussionsupporting

confidence: 92%

“…We also observed that the abundance of Pmcyclin B mRNA increased among ovarian maturation stages II (the chromatin nucleolus stage) and IV (the yolky stage), and reached peak levels at stage III. This expression pattern is consistent with that previously described for cyclin B protein in mud crabs (Li et al 2013). Up-regulation of Pmcyclin B at stage II is consistent with the proposed role of cyclin B in mitosis proliferation of oogonia (Li et al 2013).…”

Section: Discussionsupporting

confidence: 91%

“…The expression of cyclin B is highly related to cell proliferation status (Masui and Markert, 1971;Re et al, 1995). In the present study, the highest level of Pmcyclin B mRNA expression was detected in ovary, consistent with massive cell proliferation during ovarian development and maturation (Li et al, 2013). We also observed that the abundance of Pmcyclin B mRNA increased among ovarian maturation stages II (the chromatin nucleolus stage) and IV (the yolky stage), and reached peak levels at stage III.…”

Section: Discussionsupporting

confidence: 87%

“…Three bases differed from the reference sequence for Pmcyclin B by sequence alignment, but these changes were not located in the functional domain; this may have been due to sequencing error or to mutations in the gene itself. The functional domain of deduced protein was consistent among the species, suggesting that the primary structures of B-type cyclin proteins are highly conserved throughout evolution (Li et al, 2013).…”

Section: Discussionmentioning

confidence: 67%

“…Cyclins, a group of cell cycle regulatory proteins, were identified in the early 1980s as proteins that degraded at the end of each M phase cycle in sea urchin embryos (Santala et al, 2015). Subsequently, cyclins have been described in diverse organisms, from yeasts to humans (Li et al, 2013). Cyclins are classified into groups based on their structure, functional period in the cell cycle, and regulated expression (Norbury et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

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Characterization and expression analysis of a cyclin B gene from black tiger shrimp (Penaeus monodon)

Zhao¹,

Fu²,

Yang³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. The open reading frame of black tiger shrimp (Penaeus monodon) cyclin B (Pmcyclin B) was identified, based on cDNA sequence registered in GenBank (accession No. EF015590). The target sequence was 1206 bp, corresponding to 401 amino acids. Two conserved signature sequences of the cyclin B gene family were found in the Pmcyclin B deduced aa sequence. Temporal expression of Pmcyclin B in different tissues, including ovary, lymphoid organ, brain, blood, muscle, heart, gill, hepatopancreas, and intestine, were quantified by quantitative real time PCR. Messenger RNA expression levels of Pmcyclin B were greatest in the ovary, compared to other tissues (P < 0.05). Temporal expression of Pmcyclin B in the ovary at six different developmental stages was investigated by real-time PCR; no significant difference was observed (P < 0.05). Recombinant Pmcyclin B protein and its polyclonal antibody were (2015) successfully produced. Western blot analysis revealed differential expression of Pmcyclin B in ovaries in developmental stages II to IV; a positive signal (45 kDa) was observed in all ovarian stages assessed, but was most intense at stage III. Pmcyclin B protein was assessed by immunohistochemistry and was localized to the cytoplasm of prophase oocytes at stage II and enriched in the nuclei of pro-metaphase oocytes at stages III and IV. Results from this study indicate that Pmcyclin B is constitutively expressed and plays an important role in ovarian maturation in P. monodon.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 67%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Characterization and expression analysis of a cyclin B gene from black tiger shrimp (Penaeus monodon)

Zhao¹,

Fu²,

Yang³

et al. 2015

Genet. Mol. Res.

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Characterization, expression and silencing by RNAi of p53 from Penaeus monodon

et al. 2016

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The tumor suppressor p53 is a sequence-specific transcription factor, whose target genes can regulate genomic stability, the cellular response to DNA damage and cell-cycle progression. In the present study, the full-length complementary DNA (cDNA) sequence of p53 gene from Penaeus monodon (Pmp53) was cloned by the technology of rapid amplification of cDNA ends (RACE). The cDNA of Pmp53 was 2239 bp, encoding a protein of 450 amino acids with calculated molecular weight of 50.62 kDa. The temporal expression of Pmp53 in different tissues (ovary, heart, intestine, brain, muscles, stomach and gills) and different developmental stages of ovary was investigated by real-time quantitative PCR (RT-qPCR). The lowest expression level of Pmp53 was observed in the stomach, while the highest expression level was detected in the brain. During the ovary development stages, the expression level of Pmp53 reached the peak at stage III. RNA interference (RNAi) and serotonin (5-hydroxytryptamine, 5-HT) injection experiments were conducted to study the expression profile of Pmp53 and PmCDK2 (cyclin-dependent kinase 2, CDK2). Knocked down of Pmp53 by dsRNA-p53 was sequence-specific and successful. Expression levels of Pmp53 and PmCDK2 in ovary of P. monodon were significantly increased at 12-96 h post 5-HT injection. These results indicate that Pmp53 may be involved in the regulation of ovarian development of P. monodon.

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Characterization and expression of calcium-activated potassium channels (Slo) in ovary of the mud crab, Scylla paramamosain

et al. 2013

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Molecular cloning, expression profiles and subcellular localization of cyclin B in ovary of the mud crab, Scylla paramamosain

Cited by 11 publications

References 47 publications

Characterization and expression analysis of a cyclin B gene from black tiger shrimp (Penaeus monodon)

Characterization and expression analysis of a cyclin B gene from black tiger shrimp (Penaeus monodon)

Characterization, expression and silencing by RNAi of p53 from Penaeus monodon

Characterization and expression of calcium-activated potassium channels (Slo) in ovary of the mud crab, Scylla paramamosain

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