Molecular cloning, expression and tissue distribution of a chicken angiotensin II receptor

Kempf, Hervé; Moullec, Jean-Michel le; Corvol, Pierre; Gasc, Jean‐Marie

doi:10.1016/s0014-5793(96)01264-1

Cited by 22 publications

(16 citation statements)

References 18 publications

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“…46 In vivo treatment with Sar1-Ile8-Ang II decreased hematocrit levels to the same extent as fosinoprilate, during the same time window, strongly suggesting that the proerythropoietic effect of ACE specifically involved Ang II.…”

Section: Discussionmentioning

confidence: 90%

Role of the renin-angiotensin system in primitive erythropoiesis in the chick embryo

Savary

Michaud

Favier

et al. 2005

Blood

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Inactivation of the gene encoding mouse angiotensin I-converting enzyme (ACE), which converts angiotensin I into angiotensin II, results in anemia in adult animals. This anemia is corrected by angiotensin II, demonstrating the involvement of angiotensin II in adult (definitive) erythropoiesis. We investigated the possible role of the renin-angiotensin system (RAS) in primitive erythropoiesis in the yolk sac of the chicken embryo. Enzymatically active ACE was detected in the yolk sac endoderm, concomitantly with the differentiation of blood islands in the adjacent yolk sac mesoderm. The simultaneous presence of all the other components of the RAS (renin, angiotensinogen, angiotensin II receptor) in the vicinity of the blood islands suggests that this system is involved in erythropoiesis. This role was confirmed by in vivo blockade of the RAS with fosinoprilate, a specific inhibitor of chicken ACE, which decreased hematocrit by 15%. A similar decrease in hematocrit was observed following treatment with the angiotensin II receptor antagonist Sar1-Ile8-Angiotensin II, suggesting that this effect was mediated by angiotensin II. Both treatments affected hematocrit by decreasing erythroblast proliferation. Thus, the RAS, and its effector peptide angiotensin II in particular, modulates primitive erythropoiesis. IntroductionAngiotensin I converting-enzyme (ACE) is a key element of the renin-angiotensin system (RAS), an integrated system of peptides, enzymes, and membrane receptors, the principal role of which is the regulation of blood pressure and hydromineral homeostasis. This function is accomplished by generation of the octapeptide angiotensin II (Ang II) from the inactive decapeptide angiotensin I (Ang I). 1 Ang II exerts a strong vasopressor effect, but evidence is accumulating that this molecule has several other important functions: it has been found to be proinflammatory, 2 to facilitate the generation of reactive oxygen species (ROS), 3 and to possess hypertrophic and hyperplastic properties in various cell types. [4][5][6][7] The RAS also appears to regulate hematopoiesis, in both experimental models and humans. Indeed, ACE knock-out mice present isolated normochrome anemia, 8 whereas in humans, anemia and leukopenia have been reported following the initiation of treatment with high doses of ACE inhibitors. 9,10 RAS blockade with Ang II receptor antagonists or ACE inhibitors has even been used to treat patients with posttransplantation erythrocytosis or polycythemia vera. 11,12 Finally, further indirect evidence for involvement of the RAS in hematopoiesis is provided by the marked increase in plasma renin concentration reported in patients with chronic obstructive pulmonary disease accompanied by secondary erythrocytosis, 13 suggesting a common causal relationship.This demonstration of a role for ACE in the regulation of adult hematopoiesis led us to investigate the possible role of this enzyme in another important hematopoietic event: primitive hematopoiesis. We used a simple and comprehensive model: the chi...

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Section: Discussionmentioning

confidence: 90%

Role of the renin-angiotensin system in primitive erythropoiesis in the chick embryo

Savary

Michaud

Favier

et al. 2005

Blood

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“…Non-AT 1 /non-AT 2 receptors have previously been described in tissues of avian species, [40][41][42][43] and a unique Ang II binding site in human endometrium has also been described. 44 Interestingly, saralasin has been reported to be a partial agonist at the atypical AT receptor found in the nasal salt gland of Anas platyrhynchos.…”

Section: Discussionmentioning

confidence: 99%

“…Degradation products of the octapeptide Ang II (or its precursor molecules) may also exert actions on cells via non-AT 1 /non-AT 2 receptors. Thus, angiotensin (1-7) 37,43 and angiotensin IV (3-8) 47,48 have been reported to promote or inhibit DNA synthesis in a variety of cell types. It would be important to examine the effects of these analogues and their antagonists in this preparation in future studies.…”

Section: Discussionmentioning

confidence: 99%

Action of Angiotensin II on DNA Synthesis by Human Saphenous Vein in Organ Culture

2000

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Abstract-Angiotensin II (Ang II), an effector peptide of the renin-angiotensin system, has been reported to stimulate growth of blood vessels in vivo and smooth muscle cells in culture. In this study, the effect of Ang II on DNA synthesis was examined in deendothelialized human saphenous vein in organ culture. After 7 days' exposure to medium containing 0.4% fetal calf serum plus Ang II, there was a marked increase in DNA synthesis. The effect of Ang II was comparable to the response to platelet-derived growth factor. Responses to Ang II were partially inhibited by the AT 1 receptor antagonist candesartan. An AT 2 receptor antagonist, PD123319, had no effect on Ang II-induced DNA synthesis, either alone or in combination with candesartan. and inhibits neointimal growth after vascular injury in rats. 6 However, such effects may be species dependent, because ACEI has been reported to have little or no effect on the response to vascular injury in rabbits, 7 pigs, 8 or baboons. 9 In addition, an ACEI also failed to reduce the incidence of restenosis in a recent clinical trial. 10Angiotensin type 1 (AT 1 ) receptors are considered to mediate most of the effects of Ang II on vascular growth, 11 although angiotensin type 2 (AT 2 ) receptors may also contribute.12-14 Rodent vascular injury models have been reported to overexpress AT 1 receptors, 15 and an AT 1 antagonist inhibits neointimal hyperplasia in injured rat carotid arteries. 16 In contrast, an AT 1 antagonist had no effect on intimal hyperplasia in a porcine vascular injury model. 17Ang II has also been reported to promote the growth of cultured smooth muscle cells. Ang II stimulated 3 H-thymidine and 3 H-leucine incorporation in smooth muscle cells derived from human aorta and subcutaneous arteries. 18 In contrast, in human coronary smooth muscle cells, Ang II stimulated protein synthesis but not DNA synthesis. 19 In human saphenous vein-derived smooth muscle cells, Ang II induced some DNA synthesis in one study 20 but failed to stimulate proliferation or protein synthesis in another. 21 Although these data provide some evidence for trophic effects of Ang II, isolated cells may lack cell-cell and cell-matrix interactions that influence responses to growth factors. We therefore examined the effect of Ang II on DNA synthesis in human saphenous vein segments in an organ culture system in which such interactions are maintained. These studies also examined the involvements of receptor subtypes and the possible role of tyrosine kinase activation in Ang II-induced responses. Methods Clinical DetailsSegments of veins surplus to clinical requirement were obtained from 55 patients (43 men and 12 women, age range 50 to 78 years) who were undergoing coronary artery bypass surgery. Studies were in accordance with local ethics committee guidelines. Patients taking ACEI or AT 1 antagonists before surgery were excluded from the study.

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“…The following method was used to amplify specific PCR products from the cDNA pool: primers for the chicken Ang II receptor were designed using the Primer Express v2 (ABI Prism) software, based on the existing Ang II receptor sequence for the chicken (forward 679 5′ TGG CCA TAG TGC ATC CAG TG 3′ reverse 729 5′ CAA CAA ACA TGG TAC GTC GGA 3′) (Kempf et al 1996, Accession # NM 205157). Primers for the 18S rRNA were based on the existing sequence for the mouse (forward 1,275, 5′ GAC ACG GAC AGG ATT GAC AGA TTG ATA G 3′ and reverse 1,403, 5′ GTT AGC CCA GAG TCT CGT TCG TT 3′, Accession # NR 003278).…”

Section: Methodsmentioning

confidence: 99%

Maturation of the angiotensin II cardiovascular response in the embryonic White Leghorn chicken (Gallus gallus)

et al. 2010

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Angiotensin II (Ang II) is an important regulator of cardiovascular function in adult vertebrates. Although its role in regulating the adult system has been extensively investigated, the cardiovascular response to Ang II in embryonic vertebrates is relatively unknown. We investigated the potential of Ang II as a regulator of cardiovascular function in embryonic chickens, which lack central nervous system control of cardiovascular function throughout the majority of incubation. The cardiovascular response to Ang II in embryonic chickens was investigated over the final 50% of their development. Ang II produced a dose-dependent increase in arterial pressure on each day of development studied, and the response increased in intensity as development progressed. The Ang II type-1 receptor nonspecific competitive peptide antagonist [Sar1 ile8] Ang II blocked the cardiovascular response to subsequent injections of Ang II on day 21 only. The embryonic pressure response to Ang II (hypertension only) differed from that of adult chickens, in which initial hypotension is followed by hypertension. The constant level of gene expression for the Ang II receptor, in conjunction with an increasing pressure response to the peptide, suggests that two Ang II receptor subtypes are present during chicken development. Collectively, the data indicate that Ang II plays an important role in the cardiovascular development of chickens; however, its role in maintaining basal function requires further study.

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Molecular cloning, expression and tissue distribution of a chicken angiotensin II receptor

Cited by 22 publications

References 18 publications

Role of the renin-angiotensin system in primitive erythropoiesis in the chick embryo

Role of the renin-angiotensin system in primitive erythropoiesis in the chick embryo

Action of Angiotensin II on DNA Synthesis by Human Saphenous Vein in Organ Culture

Maturation of the angiotensin II cardiovascular response in the embryonic White Leghorn chicken (Gallus gallus)

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