Molecular cloning, expression, and regulation of the ovalbumin gene in pigeon oviduct epithelial cells

Abstract

ABSTRACT. The full-length pigeon ovalbumin (OVA) gene cDNA was cloned and sequenced by reverse transcription-polymerase chain reaction (RT-PCR) and rapid-amplification of cDNA ends. A 386-amino acid protein was predicted for the obtained sequence, which had 67% identity with the chicken protein. Similar to chicken OVA, the pigeon OVA gene is a non-inhibitory serine protease inhibitor. Quantitative PCR analysis revealed that pigeon OVA mRNA was highly expressed in the oviduct, and trace amounts were detected in other tissues. During the reproductive cycle, pigeon oviduct OVA mRNA expression reached its peak during the egg-laying stage, decreased with brooding, and then increased again during the squabfeeding period. Moreover, the relative OVA expression level in pigeon oviduct epithelial cells could be upregulated by a constant concentration of steroid hormones.

Temperature- vs. estrogen-induced sex determination in Caiman latirostris embryos: Both females, but with different expression patterns of key molecules involved in ovarian development

“…We observed very low expression of PR in the first stages studied in both TSD and E 2 SD female gonads, but at stage 27, the highest expression of this receptor in E 2 SD female gonads was observed. Incipient estrogen action could produce both the down-regulation of ERa and the stimulation of PR as in the female reproductive tract of mammals and avian species (Shao et al, 2007;Varayoud et al, 2008;Zhang et al, 2014). The same pattern of results is observed in E 2 SD females.…”

Salt tolerance of potato genetically engineered with the Atriplex canescens BADH gene

“…The tools used for this analysis included Prot Comp v. 9.0 (http://linux1. softberry.com/berry.phtml), MultiLoc2 (Zhang et al 2014), CELLO v. 2.5 (Yu et al 2006), and Euk-mPLoc 2.0 (Chou and Shen 2007). Additionally, the theoretical pI and Mr values were computed using the Expassy Compute pI/Mw tool (web.expasy.org/compute_pi/).…”