Molecular cloning, expression, and functional analysis of caspase-10 from Japanese flounder Paralichthys olivaceus

Effect of infectious hypodermal and haematopoietic necrosis virus (IHHNV) infection on caspase 3c expression and activity in freshwater prawn Macrobrachium rosenbergii

“…Interestingly, caspase 3 from P. monodon and Litopenaeus vannamei are not clustered together with M. rosenbergii caspase 3c, instead they are clustered together with other arthropod caspase 3 groups. As reported earlier [20,45,46], even though caspase 3 and 3c share a similar protein structure and are located in the same chromosome, however they are grouped separately. It is possible to suggest that caspase 3 and 3c may be located at the same chromosome, but at different locus.…”

“…From this phylogenetic analysis, it is possible to suggest that MrCasp3c gene belongs to caspase 3C family. According to Fernandes-Alnemri et al [45], Hadano et al [46] and Kurobe et al [20] caspase 3C and 3 shares a similar protein structure and the genes are considered to have arisen from one gene by tandem duplication because these two genes are located in the same chromosome. Therefore, it is difficult to identify and classify homologue genes into caspase 3C or 3 based on their protein structures.…”

“…Caspase 2 exhibits both the characteristics of initiator and effector of caspases and promotes apoptosis in some cells [8]. Besides that, caspase 9 functions in the stimulation of downstream effector in mitochondrial mediated pathway [16] whereas caspase 10 mainly is involved in the activation of the downstream caspases prior to its activation [20].…”

“…A few reports are available on molecular cloning and characterization of different classes of caspases including caspase 3 from white shrimp Penaeus merguiensis [3], black tiger shrimp Penaeus monodon [21,22], large yellow croaker Pseudosciaena crocea [17], Chinese mitten crab Eriocheir sinensis [23], caspase 6 from rainbow trout Oncorhynchus mykiss [6], caspase 10 from Japanese flounder Paralichthys olivaceus [20], caspase 9 from P. crocea [24] and caspase 3, 8 and 9 from European sea bass Dicentrarchus labrax [4,16,19].…”

First molecular cloning of a molluscan caspase from variously colored abalone (Haliotis diversicolor) and gene expression analysis with bacterial challenge

“…The initiator caspases are located up-stream in the caspase cascade and receive the apoptotic signals, and then transmit them to activate downstream effector caspases [4]. The effector caspases cleave a wide variety of cellular substrates, leading to the death of cells, and so function as the executioner of apoptosis [4,5].…”