Molecular cloning, expression and characterization of a novel feruloyl esterase enzyme from the symbionts of termite (Coptotermes formosanus) gut

Thulasi, A.; Bagath, M.; Kumar, Duvvuri Prasanna; Santosh, Sunil Singh; Palanivel, C.; Jose, Vazhakkala Lyju; Sampath, K. T.

doi:10.5483/bmbrep.2011.44.1.52

Cited by 18 publications

(7 citation statements)

References 26 publications

(21 reference statements)

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“…Metagenomics [Handelsman et al, 1998] provides access to novel FAEs in natural environments and leads to the identification of enzymes with potential industrial applications [Elend et al, 2006;Voget et al, 2006]. A few novel FAEs have been identified by metagenome-based methods from guts of earthworm and termite to date [Chandrasekharaiah et al, 2011;Vleltes et al, 2010;Wheeler et al, 2010].…”

Section: Introductionmentioning

confidence: 99%

Molecular Cloning, Overexpression and Characterization of a Novel Feruloyl Esterase from a Soil Metagenomic Library

Sang¹,

Li²,

Hu³

et al. 2011

Microb Physiol

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The gene estF27, encoding a protein with feruloyl esterase activity, was cloned through functional screening from a soil metagenomic library and expressed in Escherichiacoli BL21 (DE3) with high solubility. Sequence analysis showed that estF27 encoded a protein of 291 amino acids with a predicted molecular mass of 31.16 kDa. According to the substrate specificity, EstF27 was classified as a type A feruloyl esterase. EstF27 displayed optimal activity at 40°C and pH 6.8. This enzyme was stable in a broad pH range of 5.0–10.0 over 24 h, and retained more than 50% of its activity after 96 or 120 h incubation in the presence of 3 M KCl or 5 M NaCl. The enzyme activity was slightly enhanced by the addition of Mg²⁺ and Fe³⁺ at a low concentration, and completely inhibited by Cu²⁺. In the enzymatic hydrolysis of destarched wheat bran, EstF27 could release ferulic acid from it in the presence of xylanase from Thermomyces lanuginosus. Given its alkalitolerance, halotolerance and highly soluble expression, EstF27 is a promising candidate for industrial applications.

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Section: Introductionmentioning

confidence: 99%

Molecular Cloning, Overexpression and Characterization of a Novel Feruloyl Esterase from a Soil Metagenomic Library

Sang¹,

Li²,

Hu³

et al. 2011

Microb Physiol

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“…The ability of Tvms10a and Tvmz2a to release FA and diFA from the crude plant material was measured. However, these measurements have been carried out on phenolic acid-substituted xylan polysaccharide isolated from wheat straw and untreated crude cell wall materials (Bartolome et al, 1997;Chandrasekharaiah et al, 2011), and was found that there is a release of phenolic acids from the same substrates of wheat bran and spent grain in the presence of enzymes xylanase and feruloyl esterase (Tenkanen et al, 1991).…”

Section: Discussionmentioning

confidence: 97%

“…The purified proteins of Tvms10a and Tvmz2a were subjected to stepdialysis to obtain correct folding of recombinant FAE enzyme. The apparent molecular mass of the purified recombinant protein of Tvms10a (18.6 kDa) and Tvmz2a (31.2 kDa) was estimated using SDS-PAGE and MALDI-TOF, and resembled those of the analogous enzyme from symbiontes of termite (18.5 kDa) (Chandrasekharaiah et al, 2011) Clostridium stercorarium (33 kDa) (Donaghy et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Expression and biochemical characterization of two novel feruloyl esterases derived from fecal samples of Rusa unicolor and Equus burchelli

Thulasi

Vijayarani

Kumar

et al. 2012

Gene

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“…N. aquilinus feeds exclusively on wood, whereas C. fulviceps consumes more nitrogen rich organic matter, including either living or decaying plant tissues 84,85 . This difference in diet may explain the metabolic pathway profiles of each termite colony.…”

Section: Discussionmentioning

confidence: 99%

Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes

Victorica

Soria

Batista-García

et al. 2020

Sci Rep

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In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with different feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus. Our goal was to assess the microbial community compositions and metabolic capacity, and to identify genes involved in lignocellulose degradation. Individuals from both termite species contained the same five dominant bacterial phyla (Spirochaetes, Firmicutes, Proteobacteria, Fibrobacteres and Bacteroidetes) although with different relative abundances. However, detected functional capacity varied, with C. fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid metabolism, whereas N. aquilinus (a wood-feeder) gut microbiome samples were enriched in genes involved in carbohydrate metabolism and cellulose degradation. The C. fulviceps gut microbiome was enriched specifically in genes coding for debranching-and oligosaccharide-degrading enzymes. These findings suggest an association between the primary food source and the predicted categories of the enzymes present in the gut microbiomes of each species. To further investigate the termite microbiomes as sources of biotechnologically relevant glycosyl hydrolases, a putative GH10 endo-β-1,4xylanase, Xyl10E, was cloned and expressed in Escherichia coli. Functional analysis of the recombinant metagenome-derived enzyme showed high specificity towards beechwood xylan (288.1 IU/mg), with the optimum activity at 50 °C and a pH-activity range from 5 to 10. These characteristics suggest that Xy110E may be a promising candidate for further development in lignocellulose deconstruction applications.

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Molecular cloning, expression and characterization of a novel feruloyl esterase enzyme from the symbionts of termite (Coptotermes formosanus) gut

Cited by 18 publications

References 26 publications

Molecular Cloning, Overexpression and Characterization of a Novel Feruloyl Esterase from a Soil Metagenomic Library

Molecular Cloning, Overexpression and Characterization of a Novel Feruloyl Esterase from a Soil Metagenomic Library

Expression and biochemical characterization of two novel feruloyl esterases derived from fecal samples of Rusa unicolor and Equus burchelli

Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes

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