Molecular cloning, expression analysis of interleukin 17D (cysteine knot cytokine) from Amphiprion clarkii and their functional characterization and NFκB pathway activation using FHM cells

Liyanage, D.S.; Omeka, W.K.M.; Nadarajapillai, Kishanthini; Lim, Chaehyeon; Yang, Hyerim; Choi, Ji Young; Kim, Kyong Min; Noh, Jae Koo; Jeong, Taehyug; Kwon, Hyukjae

doi:10.1016/j.fsi.2022.05.047

Cited by 4 publications

(1 citation statement)

References 34 publications

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“…Functional enrichment analysis of candidate genes identified through population selection analysis revealed significant enrichment of pathways related to antimicrobial peptide, humoral immunity, and apoptosis in genes within candidate intervals determined through F ST analysis. Seven of these pathways were associated with antimicrobial peptide or antimicrobial humoral response, which play a crucial role in immunity by disrupting bacterial membranes [52,53] and activating the NFκB pathway to recruit antimicrobial peptides and pro-inflammatory cytokines to initiate defense mechanisms [54]. In addition, ROD analysis identified candidate genes with significantly increased likelihood of being associated with immunity, such as inflammatory response, in the GO terms, suggesting potential differences in immune function between Russian and Chinese groups of A. japonicus.…”

Section: Selective Characterization Of the Genome Of Chinese And Russ...mentioning

confidence: 99%

Whole-genome resequencing reveals genetic differences and the genetic basis of parapodium number in Russian and Chinese Apostichopus japonicus

et al. 2023

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Background Apostichopus japonicus is an economically important species in the global aquaculture industry. Russian A. japonicus, mainly harvested in the Vladivostok region, exhibits significant phenotypic differentiation, including in many economically important traits, compared with Chinese A. japonicus owing to differences in their habitat. However, both the genetic basis for the phenotypic divergence and the population genetic structure of Russian and Chinese A. japonicus are unknown. Result In this study, 210 individuals from seven Russian and Chinese A. japonicus populations were sampled for whole-genome resequencing. The genetic structure analysis differentiated the Russian and Chinese A. japonicus into two groups. Population genetic analyses indicated that the Russian population showed a high degree of allelic linkage and had undergone stronger positive selection compared with the Chinese populations. Gene ontology terms enriched among candidate genes with group selection analysis were mainly involved in immunity, such as inflammatory response, antimicrobial peptides, humoral immunity, and apoptosis. Genome-wide association analysis yielded eight single-nucleotide polymorphism loci significantly associated with parapodium number, and these loci are located in regions with a high degree of genomic differentiation between the Chinese and Russia populations. These SNPs were associated with five genes. Gene expression validation revealed that three of these genes were significantly differentially expressed in individuals differing in parapodium number. AJAP08772 and AJAP08773 may directly affect parapodium production by promoting endothelial cell proliferation and metabolism, whereas AJAP07248 indirectly affects parapodium production by participating in immune responses. Conclusions This study, we performed population genetic structure and GWAS analysis on Chinese and Russian A. japonicus, and found three candidate genes related to the number of parapodium. The results provide an in-depth understanding of the differences in the genetic structure of A. japonicus populations in China and Russia, and provide important information for subsequent genetic analysis and breeding of this species.

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Section: Selective Characterization Of the Genome Of Chinese And Russ...mentioning

confidence: 99%

Whole-genome resequencing reveals genetic differences and the genetic basis of parapodium number in Russian and Chinese Apostichopus japonicus

et al. 2023

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Galectin-8-like isoform X1 mediates antibacterial, antiviral, and antioxidant responses in red-lip mullet (Planiliza haematocheilus) through positive modulation of pro-inflammatory cytokine, chemokine, and enzymatic antioxidant activity

Warnakula,

Udayantha,

Liyanage

et al. 2024

Developmental & Comparative Immunology

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Transcriptome analysis of response mechanism to Microcystin-LR and microplastics stress in Asian clam (Corbicula fluminea)

Zhang

Wang

Cheng

et al. 2023

Fish & Shellfish Immunology

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Molecular cloning, expression analysis of interleukin 17D (cysteine knot cytokine) from Amphiprion clarkii and their functional characterization and NFκB pathway activation using FHM cells

Cited by 4 publications

References 34 publications

Whole-genome resequencing reveals genetic differences and the genetic basis of parapodium number in Russian and Chinese Apostichopus japonicus

Whole-genome resequencing reveals genetic differences and the genetic basis of parapodium number in Russian and Chinese Apostichopus japonicus

Galectin-8-like isoform X1 mediates antibacterial, antiviral, and antioxidant responses in red-lip mullet (Planiliza haematocheilus) through positive modulation of pro-inflammatory cytokine, chemokine, and enzymatic antioxidant activity

Transcriptome analysis of response mechanism to Microcystin-LR and microplastics stress in Asian clam (Corbicula fluminea)

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