Molecular cloning, characterization, expression and enzyme activity of catalase from planarian Dugesia japonica in response to environmental pollutants

Zhang, He-Cai; Ma, Ke; Yang, Yujuan; Shi, Chenxi; Chen, Guangwen; Liu, Dezeng

doi:10.1016/j.ecoenv.2018.08.083

Cited by 28 publications

(10 citation statements)

References 38 publications

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“…Antioxidant responses can also be defined as the induction of antioxidant enzymes in the process mediated by the Keap1-Nrf2 system [ 39 ]. Among all the antioxidant enzymes, superoxide dismutase (SOD) is an antioxidant metal enzyme that can catalyze the disproportionation of superoxide anion radicals to generate oxygen and hydrogen peroxide; Catalase (CAT) is a resident enzyme of peroxisomes, decomposing hydrogen oxide into oxygen and water [ 40 ]; Glutathione peroxidase (GSH-Px) is an important peroxide decomposing enzyme widely present in the body [ 41 ]. Fig.…”

Section: Resultsmentioning

confidence: 99%

Antioxidant Activity of Novel Casein-Derived Peptides with Microbial Proteases as Characterized via Keap1-Nrf2 Pathway in HepG2 Cells

Zhao

CUI

Bai

et al. 2021

J. Microbiol. Biotechnol.

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Casein-derived antioxidant peptides by using microbial proteases have gained increasing attention. Combination of two microbial proteases, Protin SD-NY10 and Protease A “Amano” 2SD, was employed to hydrolyze casein to obtain potential antioxidant peptides that were identified by LCMS/ MS, chemically synthesized and characterized in a oxidatively damaged HepG2 cell model. Four peptides, YQLD, FSDIPNPIGSEN, FSDIPNPIGSE, YFYP were found to possess high 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging ability. Evaluation with HepG2 cells showed that the 4 peptides at low concentrations (< 1.0 mg/ml) protected the cells against oxidative damage. The 4 peptides exhibited different levels of antioxidant activity by stimulating mRNA and protein expression of the antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px), as well as nuclear factor erythroid-2-related factor 2 (Nrf2), but decreasing the mRNA expression of Kelch-like ECH-associated protein 1 (Keap1). Furthermore, these peptides decreased production of reactive oxygen species (ROS) and malondialdehyde (MDA), but increased glutathione (GSH) production in HepG2 cells. Therefore, the 4 casein-derived peptides obtained by using microbial proteases exhibited different antioxidant activity by activating the Keap1-Nrf2 signaling pathway, and they could serve as potential antioxidant agents in functional foods or pharmaceutic preparation.

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Section: Resultsmentioning

confidence: 99%

Antioxidant Activity of Novel Casein-Derived Peptides with Microbial Proteases as Characterized via Keap1-Nrf2 Pathway in HepG2 Cells

Zhao

CUI

Bai

et al. 2021

J. Microbiol. Biotechnol.

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“…As mentioned before, HO • is chemically very reactive, so the first line of defense of parasitic flatworms is to prevent its production, the controlled reduction of H 2 O 2 to H 2 O being vital. The classic enzyme that carries out this reaction in aerobic organisms is catalase (CAT), which is absent in the genomes of parasitic flatworms [ 92 , 133 ] but is conserved in their free-living counterparts [ 134 ]. Hence, these parasites depend on enzymes glutathione peroxidase (GPx) and peroxiredoxin (Prx) in addition to the superoxide dismutase (SOD) necessary for O 2 •− scavenging.…”

Section: Adaptations Of Parasitic Flatworms To Changes In Oxygen Tensionmentioning

confidence: 99%

Evolutionary Adaptations of Parasitic Flatworms to Different Oxygen Tensions

2022

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During the evolution of the Earth, the increase in the atmospheric concentration of oxygen gave rise to the development of organisms with aerobic metabolism, which utilized this molecule as the ultimate electron acceptor, whereas other organisms maintained an anaerobic metabolism. Platyhelminthes exhibit both aerobic and anaerobic metabolism depending on the availability of oxygen in their environment and/or due to differential oxygen tensions during certain stages of their life cycle. As these organisms do not have a circulatory system, gas exchange occurs by the passive diffusion through their body wall. Consequently, the flatworms developed several adaptations related to the oxygen gradient that is established between the aerobic tegument and the cellular parenchyma that is mostly anaerobic. Because of the aerobic metabolism, hydrogen peroxide (H2O2) is produced in abundance. Catalase usually scavenges H2O2 in mammals; however, this enzyme is absent in parasitic platyhelminths. Thus, the architecture of the antioxidant systems is different, depending primarily on the superoxide dismutase, glutathione peroxidase, and peroxiredoxin enzymes represented mainly in the tegument. Here, we discuss the adaptations that parasitic flatworms have developed to be able to transit from the different metabolic conditions to those they are exposed to during their life cycle.

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“…Colorimetric assays can be used to quantify the activity of hydrolases, oxidases, and other enzymes in homogenates of exposed planarians during neurodevelopment or in adulthood. The activity of oxidative stress related enzymes, such as catalase and superoxide dismutase, has been a popular molecular biomarker of toxicity in planarians (Zhang, Zhang, Yi, and Zhao, 2014, 2018). Additionally, the activity of neuronal enzymes, such as acetylcholinesterase (AChE), an important toxicological target (Russom, LaLone, Villeneuve, and Ankley, 2014), have been quantified (Hagstrom et al., 2018; Ireland, Zhang, et al., 2022; Li, 2008).…”

Section: Mechanistic Validation and Tools For High‐content Studiesmentioning

confidence: 99%

New Worm on the Block: Planarians in (Neuro)Toxicology

Ireland

Collins

2022

Current Protocols

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Traditional mammalian testing is too time‐ and cost‐intensive to keep up with the large number of environmental chemicals needing assessment. This has led to a dearth of information about the potential adverse effects of these chemicals, especially on the developing brain. Thus, there is an urgent need for rapid and cost‐effective neurotoxicity and developmental neurotoxicity testing. Because of the complexity of the brain, metabolically competent organismal models are necessary to understand the effects of chemicals on nervous system development and function on a systems level. In this overview, we showcase asexual freshwater planarians as an alternative invertebrate (“non‐animal”) organismal model for neurotoxicology research. Planarians have long been used to study the effects of chemicals on regeneration and behavior. But they have only recently moved back into the spotlight because modern molecular and computational approaches now enable quantitative high‐content and high‐throughput toxicity studies. Here, we present a short history of the use of planarians in toxicology research, highlight current techniques to measure toxicity qualitatively and quantitatively in planarians, and discuss how to further promote this non‐animal organismal system into mainstream toxicology research. The articles in this collection will help work towards this goal by providing detailed protocols that can be adopted by the community to standardize planarian toxicity testing. © 2022 Wiley Periodicals LLC.

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Molecular cloning, characterization, expression and enzyme activity of catalase from planarian Dugesia japonica in response to environmental pollutants

Cited by 28 publications

References 38 publications

Antioxidant Activity of Novel Casein-Derived Peptides with Microbial Proteases as Characterized via Keap1-Nrf2 Pathway in HepG2 Cells

Antioxidant Activity of Novel Casein-Derived Peptides with Microbial Proteases as Characterized via Keap1-Nrf2 Pathway in HepG2 Cells

Evolutionary Adaptations of Parasitic Flatworms to Different Oxygen Tensions

New Worm on the Block: Planarians in (Neuro)Toxicology

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