Molecular cloning, characterization and expression of a jasmonate biosynthetic pathway gene encoding allene oxide cyclase from <i>Camptotheca acuminata</i>

Pi, Yan; Liao, Zhihua; Jiang, Keji; Huang, Beibei; Deng, Z. G.; Zhao, Dongyuan; Zeng, Hainian; Sun, Xiaofen; Tang, Kexuan

doi:10.1042/bsr20060001

Cited by 8 publications

(4 citation statements)

References 31 publications

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“…The alignment of AOC sequences showed a high degree of conservation in the functional region, rather than in the transit peptide portion [18].…”

Section: Discussionmentioning

confidence: 99%

“…There are indirect lines of evidence, indicating that camptothecin is involved in plant defense, and some of the biosynthetic pathway genes of camptothecin are induced by JA [17]. Previously, a full-length cDNA of AOC from C. acuminata (named CaAOC) was isolated, coding a protein of 26.8 kDa that was deduced to localize in chloroplasts [18].…”

Section: Introductionmentioning

confidence: 99%

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Allene Oxide Cyclase from Camptotheca acuminata Improves Tolerance Against Low Temperature and Salt Stress in Tobacco and Bacteria

Jiang

Wang

et al. 2008

Mol Biotechnol

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Allene oxide cyclase (AOC, E 5.3.99.6) is an essential enzyme in jasmonate (JA) biosynthetic pathway. An AOC gene (defined as CaAOC, Database Accession No. AY863428) had been isolated from Camptotheca acuminata in previous work. Real-time quantitative PCR analysis indicated that mRNA expression of CaAOC was induced by salt stress (120 mM NaCl) and low temperature (4 degrees C). In order to further investigate the role of AOC gene in the processes, CaAOC was introduced into tobacco via Agrobacterium tumefaciens, and the transgenic lines were subjected to the examination of tolerance against salt stress and low temperature. Under salt stress, the chlorophyll content in transgenic tobacco was higher than that of in the wild plants. The electrolyte leakage test revealed that transgenic tobacco plants were more resistant to low temperature over control. Furthermore, 5'-truncated CaAOC was inserted into pET30 and then expressed in Escherichia coli strain BL21DE3 (pLysS). Interestingly, the transformants could grow on 2YT agar containing 400 mM NaCl. Although these mechanisms are not clear yet, this study suggested that CaAOC could not only be a potential target gene in the engineering of plants and bacteria for improved endurance against salt stress, but also be quite useful in enhancing plant tolerance to cold.

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“…The alignment of AOC sequences showed a high degree of conservation in the functional region, rather than in the transit peptide portion [18].…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Allene Oxide Cyclase from Camptotheca acuminata Improves Tolerance Against Low Temperature and Salt Stress in Tobacco and Bacteria

Jiang

Wang

et al. 2008

Mol Biotechnol

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“…The PCR amplification program was as follows: 50°C for 2 min; 95°C for 30 sec; 40 cycles of 95°C for 3 sec and 62°C for 40 sec; followed by a melting-curve program of 55°C to 85°C, with a 5-sec hold at each temperature. The gene expression patterns of all genes were normalized to an internal reference ( 18S rRNA) [53]. The relative gene expression analysis was performed using BIO-RAD IQ™5 optical system software version 2.0 with the 2 -ΔΔCt method.…”

Section: Methodsmentioning

confidence: 99%

Pyrosequencing of the Camptotheca acuminata transcriptome reveals putative genes involved in camptothecin biosynthesis and transport

et al. 2011

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BackgroundCamptotheca acuminata is a Nyssaceae plant, often called the "happy tree", which is indigenous in Southern China. C. acuminata produces the terpenoid indole alkaloid, camptothecin (CPT), which exhibits clinical effects in various cancer treatments. Despite its importance, little is known about the transcriptome of C. acuminata and the mechanism of CPT biosynthesis, as only few nucleotide sequences are included in the GenBank database.ResultsFrom a constructed cDNA library of young C. acuminata leaves, a total of 30,358 unigenes, with an average length of 403 bp, were obtained after assembly of 74,858 high quality reads using GS De Novo assembler software. Through functional annotation, a total of 21,213 unigenes were annotated at least once against the NCBI nucleotide (Nt), non-redundant protein (Nr), Uniprot/SwissProt, Kyoto Encyclopedia of Genes and Genomes (KEGG), and Arabidopsis thaliana proteome (TAIR) databases. Further analysis identified 521 ESTs representing 20 enzyme genes that are involved in the backbone of the CPT biosynthetic pathway in the library. Three putative genes in the upstream pathway, including genes for geraniol-10-hydroxylase (CaPG10H), secologanin synthase (CaPSCS), and strictosidine synthase (CaPSTR) were cloned and analyzed. The expression level of the three genes was also detected using qRT-PCR in C. acuminata. With respect to the branch pathway of CPT synthesis, six cytochrome P450s transcripts were selected as candidate transcripts by detection of transcript expression in different tissues using qRT-PCR. In addition, one glucosidase gene was identified that might participate in CPT biosynthesis. For CPT transport, three of 21 transcripts for multidrug resistance protein (MDR) transporters were also screened from the dataset by their annotation result and gene expression analysis.ConclusionThis study produced a large amount of transcriptome data from C. acuminata by 454 pyrosequencing. According to EST annotation, catalytic features prediction, and expression analysis, novel putative transcripts involved in CPT biosynthesis and transport were discovered in C. acuminata. This study will facilitate further identification of key enzymes and transporter genes in C. acuminata.

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“…Soybean GmAOC1 and GmAOC2 also exhibited abundant mRNA transcripts in roots, while GmAOC3 and GmAOC4 showed relatively higher expression in leaves and stems, respectively [7]. Camptotheca acuminata CaAOC is constitutively expressed in various organs, with the highest expression level in leaves [14]. In Arabidopsis, AtAOC1, AtAOC2, and AtAOC3 promoters exhibit high activities in the leaves, while only AtAOC3 and AtAOC4 show promoter activity in roots [15].…”

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confidence: 99%

Identification and Expression Analysis of Two allene oxide cyclase (AOC) Genes in Watermelon

Guang

Yang

et al. 2019

Agriculture

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Allene oxide cyclase (AOC, EC 5.3.99.6) catalyzes the most important step in the jasmonic acid (JA) biosynthetic pathway and mediates plant defense response to a wide range of biotic and abiotic stresses. In this study, two AOC genes were identified from watermelon. Sequence analysis revealed that each of ClAOC1 and ClAOC2 contained an allene oxide cyclase domain and comprised eight highly conserved β-strands, which are the typical characteristics of AOC proteins. Phylogenetic analysis showed that ClAOC1 and ClAOC2 were clustered together with AOCs from dicotyledon, with the closest relationships with JcAOC from Jatropha curcas and Ljaoc1 from Lotus japonicus. Different intron numbers were observed in ClAOC1 and ClAOC2, which may result in their functional divergence. qRT-PCR analysis revealed that ClAOC1 and ClAOC2 have specific and complex expression patterns in multiple organs and under hormone treatments. Both ClAOC1 and ClAOC2 displayed the highest transcriptional levels in stem apex and fruit and exhibited relatively lower expression in stem. JA, salicylic acid (SA), and ethylene (ET) could enhance the expression of ClAOC1 and ClAOC2, particularly that of ClAOC2. Red light could induce the expression of ClAOC2 in root-knot nematode infected leaf and root of watermelon, indicating that ClAOC2 might play a primary role in red light-induced resistance against root-knot nematodes through JA signal pathway. These findings provide important information for further research on AOC genes in watermelon.Agriculture 2019, 9, 225 2 of 12 AOC genes were found to have specific and complicated tissue expression patterns in different plants. In barley, HvAOC mRNA accumulation is abundant in root tip, scutellar node, and leaf base [13]. Soybean GmAOC1 and GmAOC2 also exhibited abundant mRNA transcripts in roots, while GmAOC3 and GmAOC4 showed relatively higher expression in leaves and stems, respectively [7]. Camptotheca acuminata CaAOC is constitutively expressed in various organs, with the highest expression level in leaves [14]. In Arabidopsis, AtAOC1, AtAOC2, and AtAOC3 promoters exhibit high activities in the leaves, while only AtAOC3 and AtAOC4 show promoter activity in roots [15]. These results suggest that AOC genes are involved in the regulation of multiple plant developmental processes.The expression of jasmonate biosynthetic pathway genes, including AOCs, is regulated by JA, and overexpression or suppression of these genes can greatly affect the JA levels in plants. For example, a severe deficiency of jasmonate was found in two AOC mutants (cpm2 and hebiba) of rice [12], and partial suppression of MtAOC1 in hairy roots of Medicago truncatula also resulted in lower JA levels in mycorrhizal roots [16]. In addition, elevated JA levels were found in transgenic plants overexpressing AOC genes from different plants, such as AaAOC from Artemisia annua [5], TaAOC1 from wheat [17], and GmAOC3 from soybean [18]. JA and its related compounds are involved in plant defense reactions against biotic and abiotic stresses, and th...

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Molecular cloning, characterization and expression of a jasmonate biosynthetic pathway gene encoding allene oxide cyclase from Camptotheca acuminata

Cited by 8 publications

References 31 publications

Allene Oxide Cyclase from Camptotheca acuminata Improves Tolerance Against Low Temperature and Salt Stress in Tobacco and Bacteria

Allene Oxide Cyclase from Camptotheca acuminata Improves Tolerance Against Low Temperature and Salt Stress in Tobacco and Bacteria

Pyrosequencing of the Camptotheca acuminata transcriptome reveals putative genes involved in camptothecin biosynthesis and transport

Identification and Expression Analysis of Two allene oxide cyclase (AOC) Genes in Watermelon

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