Molecular cloning, characterization and expression of immunoglobulin D on pathogen challenge and pathogen associated molecular patterns stimulation in freshwater carp, Catla catla

Abstract

The primordial immunoglobulin class, IgD, was the first non-IgM isotype discovered in teleosts. The crucial roles of IgM and IgZ in imparting systemic and mucosal immunity, respectively, in various fish species have been widely established. However, the putative function of a unique IgD isotype during pathogenic invasions has not been well explored. The present study reports the existence of an IgD ortholog in freshwater carp, Catla catla, and further evaluates its differential expression profile in response to bacterial, parasitic and viral antigenic exposure and pathogen associated molecular patterns (PAMPs) stimulation. The IgD of C. catla (CcIgD) cDNA sequence was found to encode 226 amino acids and confirmed homology with heavy chain delta region of Cyprinidae family members. Phylogenetic analysis of CcIgD exhibited greatest similarity with Ctenopharyngodon idella. qRT-PCR analysis revealed significant upregulation (P < 0.001) of IgD gene expression in kidney with respect to other tissues at 24 hr post-Aeromonas hydrophila challenge. CcIgD gene expression in skin was enhanced following Streptococcus uberis infection and in blood following Argulus infection and inactivated rhabdoviral antigen stimulation. Further, the treatment of bacterial and viral products (PAMPs) also triggered significant (P < 0.05) increases in CcIgD mRNA expression in kidney. These findings indicate the functional importance of teleost IgD in orchestrating tissue specific neutralization of antigens on stimulation with different pathogens and PAMPs.

Caspase-8 in Labeo rohita is evolutionary conserved and is activated in Aeromonas hydrophila and Edwardsiella tarda infection and rhabdovirus vaccination

“…Although, SVCV primarily affects fishes of the Cyprinidae family (Ahne et al, 2002) but VHSV infects a variety of fish species belonging to various families (Al-Hussinee et al, 2011). To investigate the response of caspase-8 gene in rhabdovirus infections, rohu fingerlings were injected with an inactivated rhabdovirus vaccine of animals containing RNA viral genome (Basu et al, 2016;Banerjee et al, 2017). The result showed significant (p < 0.05) increase in caspase-8 gene expression in all tested organs, and the most significant induction was noted in the spleen at 72 h post-injection.…”

Lrcasp9 shares similarity in structural motifs with human caspase-9 and is activated following bacterial infection and anti-viral vaccination

“…SVCV primarily affects cyprinid fishes (Ahne et al 2002) but the host range of VHSV is very wide (Al-Hussinee et al 2011). To understand the response of Caspase-9 in rhabdoviral infections, inactivated rhabdoviral vaccines of animals were injected into rohu fingerlings (Basu et al 2016;Banerjee et al 2017) and the data revealed significant increase in caspase-9 gene expression in gill, liver and spleen. In large yellow croaker, caspase-9 gene expression was also induced in spleen and kidney following poly I:C or bacterial vaccine stimulation, and it was highest at 48 h and then decreased at 72 h (Mu et al 2010).…”

Molecular cloning, characterization and expression analysis of MHCI and chemokines CXCR3 and CXCR4 gene from freshwater carp,Catla catla

“…Healthy fish were parted into two groups: control and challenged. Challenged group was infected with Aeromonas hydrophila (ATCC 35654) and Streptococcus uberis (ATCC 700407) as described by Banerjee et al . The control fish were injected with 100 µl of phosphate buffer saline (PBS) maintained in the other tank.…”

“…Challenged group was infected with Aeromonas hydrophila (ATCC 35654) and Streptococcus uberis (ATCC 700407) as described by Banerjee et al. 28 The control fish were injected with 100 µl of phosphate buffer saline (PBS) maintained in the other tank. After 24, 48 and 72 hr, the challenged fish along with control group were anesthetized using tricane methane sulphonate (Sigma-Aldrich, USA) and sacrificed for isolating the immunologically significant tissues such as liver, spleen, intestine, kidney, gill, skin and blood.…”