Molecular cloning, characterization and expression analysis of metallothionein in the liver of the teleost Acrossocheilus fasciatus exposed to cadmium chloride

Liu, Guo-Di; Sheng, Zhang; Hou, Cong-Cong; Ni, Jie; Han, Yingli; Wang, Youfa; Zhou, Yang; Fu, Su-Yan; Zhu, Junquan

doi:10.1016/j.etap.2017.03.020

Cited by 8 publications

(3 citation statements)

References 74 publications

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“…For instance, in Oncorhynchus mykiss , Cu 2+ uptake increased during the 1–2 h under radiolabelled copper exposure, and after 2 h, Cu 2+ level significantly decreased in the gill 39 . A similar tendency was found in Acrossocheilus fasciatus 40 , in which the expression level of zinc-finger BED domain-containing protein (Zbed) was significantly decreased after exposure to Cd 2+ , which is also consistent with what has been observed in Mytilus galloprovincialis exposed to Cu 2+ 41 . In contrast, hemocyanin-like protein, a crucial immune protein in arthropods 42 – 44 , had significantly increased expression after exposure to Cd 2+ .…”

Section: Discussionsupporting

confidence: 87%

Comparative transcriptome analysis of the gills and hepatopancreas from Macrobrachium rosenbergii exposed to the heavy metal Cadmium (Cd2+)

Jiang

et al. 2021

Sci Rep

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Heavy metal Cadmium (Cd2+) pollution has become a severe environmental problem for aquatic organisms. In crustaceans, gills (Gi) and hepatopancreas (Hp) play a vital role in the toxicology. However, in Macrobrachium rosenbergill, there are few researches about gill and hepatopancreases responding to Cd2+ stress at a molecular level. In this study, transcriptomic analysis was applied to characterize gene expression profiles of gills and hepatopancreas of M. rosenbergill after Cd2+ exposure for 0 h, 3 h and 3 d. Six cDNA libraries (Gi 0 h, Gi 3 h, Gi 3 d, Hp 0 h, Hp 3 h, and Hp 3 d) were constructed and a total of 66,676 transcripts and 48,991 unigenes were annotated. Furthermore, differentially expressed genes (DEGs) were isolated by comparing the Cd2+ treated time-point libraries (3 h and 3 d group) with the control library (0 h group). The results showed that most of the DEGs were down-regulated after Cd2+ exposure and the number of DEGs among gill groups were significantly higher than those among hepatopancreas groups. GO functional and KEGG pathway analysis suggested many key DEGs in response to the Cd2+ stress, such as metallothionein and Hemocyanin. Additionally, a total of six DEGs were randomly selected to further identify their expressional profile by qPCR. The results indicated that these DEGs were involved in the response to Cd2+. This comparative transcriptome provides valuable molecular information on the mechanisms of responding to Cd2+ stress in M. rosenbergii, which lays the foundation for further understanding of heavy metal stress.

show abstract

Section: Discussionsupporting

confidence: 87%

Comparative transcriptome analysis of the gills and hepatopancreas from Macrobrachium rosenbergii exposed to the heavy metal Cadmium (Cd2+)

Jiang

et al. 2021

Sci Rep

View full text Add to dashboard Cite

show abstract

“…For instance, in Oncorhynchus mykiss, Cu 2+ uptake increased during the 1-2 h under radiolabelled copper expose, and after 2 h, Cu 2+ level signi cantly decreased in gill 37 . A similar tendency was found in Acrossocheilus fasciatus 38 , in which the expression level of zinc-nger BED domain-containing protein (Zbed) was signi cantly decreased after exposure to Cd 2+ , which is also consistent with what has been observed in Mytilus galloprovincialis exposed to Cu 2+ 39 . In contrast, hemocyanin-like protein, a crucial immune protein in arthropods [40][41][42] , was signi cantly increased expression after exposure to Cd 2+ .…”

Section: Discussionsupporting

confidence: 85%

Comparative transcriptome analysis of the gills and hepatopancreas from Macrobrachium rosenbergii exposed to the heavy metal Cadmium (Cd 2+ )

Jiang

et al. 2021

Preprint

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Heavy metal Cadmium (Cd2+) pollution has become a severe environmental problem for aquatic organisms. In crustaceans, gills and hepatopancreas play a vital role in the toxicology. However, in Macrobrachium rosenbergill, there are few researches about gill and hepatopancreases responding to Cd2+ stress at a molecular level. In this study, transcriptomic analysis was applied to characterize gene expression profiles of gills and hepatopancreas of M. rosenbergill after Cd2+ exposure for 0 h, 3 h and 3 d. Six cDNA libraries (Gi 0 h, Gi 3 h, Gi 3 d, Hp 0 h, Hp 3 h, and Hp 3 d) were constructed and a total of 66,676 transcripts and 48,991 unigenes were annotated. Furthermore, differentially expressed genes (DEGs) were isolated by comparing the Cd2+ treated time-point libraries (3 h and 3 d group) with the control library (0 h group). The results showed that most of the DEGs were down-regulated after Cd2+ exposure and the number of DEGs among gill groups were significantly higher than those among hepatopancreas groups. GO functional and KEGG pathway analysis suggested many key DEGs in response to the Cd2+ stress, such as metallothionein and Hemocyanin. Additionally, a total of six DEGs were randomly selected to further identify their expressional profile by qPCR. The results indicated that these DEGs were involved in the response to Cd2+. This comparative transcriptome provides valuable molecular information on the mechanisms of responding to Cd2+ stress in M. rosenbergii, which lays the foundation for further understanding of heavy metals stress.

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“…Acrossocheilus fasciatus is an important economic and ornamental fish, which is widely distributed in the gravel deposits of rivers and streams in southern China [ 21 ]. In recent years, the distribution range and quantity of A. fasciatus have declined rapidly as a result of overfishing and the degradation of the aquatic environment, and artificial breeding has become a new option.…”

Section: Introductionmentioning

confidence: 99%

Integrated mRNA and miRNA Expression Profile Analysis of Female and Male Gonads in Acrossocheilus fasciatus

Wei

Yaqoob

et al. 2022

Biology

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MicroRNAs (miRNAs) are regarded as key regulators in gonadal development and sex determination in diverse organisms. However, the functions of miRNAs in gonads of Acrossocheilus fasciatus, an economically important freshwater species in the south of China, are still unclear. Here, high-throughput sequencing was performed to investigate the mRNA and miRNAs on gonads of A. fasciatus. In total, 49,447 unigenes were obtained, including 11,635 differentially expressed genes (DEGs), among which 4147 upregulated genes and 7488 downregulated genes in the testis compared to the ovary, while 300 (237 known, and 63 novel) miRNAs with 36 differentially expressed miRNAs (DEMs) were identified, from which 17 upregulated and 19 downregulated DEMs. GO and KEGG enrichment analysis were performed to analyze the potential biological functions of DEGs and DEMs. Using qRT-PCR, 9 sex-related genes and 9 miRNAs were selected to verify the sequencing data. By dual-luciferase reporter assay, miR-22a-5p and miR-22b-5p interaction with piwil1, and miR-10d-5p interaction with piwil2 were identified. These findings could provide a reference for miRNA-regulated sex control of A. fasciatus and may reveal new insights into aquaculture and breeding concepts.

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Molecular cloning, characterization and expression analysis of metallothionein in the liver of the teleost Acrossocheilus fasciatus exposed to cadmium chloride

Cited by 8 publications

References 74 publications

Comparative transcriptome analysis of the gills and hepatopancreas from Macrobrachium rosenbergii exposed to the heavy metal Cadmium (Cd2+)

Comparative transcriptome analysis of the gills and hepatopancreas from Macrobrachium rosenbergii exposed to the heavy metal Cadmium (Cd2+)

Comparative transcriptome analysis of the gills and hepatopancreas from Macrobrachium rosenbergii exposed to the heavy metal Cadmium (Cd 2+ )

Integrated mRNA and miRNA Expression Profile Analysis of Female and Male Gonads in Acrossocheilus fasciatus

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